Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition

Autores
Pallé, Anna; Montero, Marta; Fernendez, Silvia; Tezanos, Patricia; De las Heras, Juan; Luskey, Valerie; Birnbaumer, Lutz; Zufall, Frank; Chamero, Pablo; Trejo, José Luis
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pheromone detection by the vomeronasal organ (VNO) mediates important social behaviors across different species, including aggression and sexual behavior. However, the relationship between vomeronasal function and social hierarchy has not been analyzed reliably. We evaluated the role of pheromone detection by receptors expressed in the apical layer of the VNO such as vomeronasal type 1 receptors (V1R) in dominance behavior by using a conditional knockout mouse for G protein subunit Gαi2, which is essential for V1R signaling. We used the tube test as a model to analyze the within-a-cage hierarchy in male mice, but also as a paradigm of novel territorial competition in animals from different cages. In absence of prior social experience, Gαi2 deletion promotes winning a novel social competition with an unfamiliar control mouse but had no effect on an established hierarchy in cages with mixed genotypes, both Gαi2−/− and controls. To further dissect social behavior of Gαi2−/− mice, we performed a 3-chamber sociability assay and found that mutants had a slightly altered social investigation. Finally, gene expression analysis in the medial prefrontal cortex (mPFC) for a subset of genes previously linked to social status revealed no differences between group-housed Gαi2−/− and controls. Our results reveal a direct influence of pheromone detection on territorial dominance, indicating that olfactory communication involving apical VNO receptors like V1R is important for the outcome of an initial social competition between two unfamiliar male mice, whereas final social status acquired within a cage remains unaffected. These results support the idea that previous social context is relevant for the development of social hierarchy of a group. Overall, our data identify two context-dependent forms of dominance, acute and chronic, and that pheromone signaling through V1R receptors is involved in the first stages of a social competition but in the long term is not predictive for high social ranks on a hierarchy.
Fil: Pallé, Anna. Consejo Superior de Investigaciones Científicas; España
Fil: Montero, Marta. Consejo Superior de Investigaciones Científicas; España
Fil: Fernendez, Silvia. Consejo Superior de Investigaciones Científicas; España
Fil: Tezanos, Patricia. Consejo Superior de Investigaciones Científicas; España
Fil: De las Heras, Juan. Consejo Superior de Investigaciones Científicas; España
Fil: Luskey, Valerie. Consejo Superior de Investigaciones Científicas; España
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Zufall, Frank. Universitat Saarland; Alemania
Fil: Chamero, Pablo. Centre National de la Recherche Scientifique; Francia
Fil: Trejo, José Luis. Consejo Superior de Investigaciones Científicas; España
Materia
Gαi2-/-
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141692
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competitionPallé, AnnaMontero, MartaFernendez, SilviaTezanos, PatriciaDe las Heras, JuanLuskey, ValerieBirnbaumer, LutzZufall, FrankChamero, PabloTrejo, José LuisGαi2-/-https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pheromone detection by the vomeronasal organ (VNO) mediates important social behaviors across different species, including aggression and sexual behavior. However, the relationship between vomeronasal function and social hierarchy has not been analyzed reliably. We evaluated the role of pheromone detection by receptors expressed in the apical layer of the VNO such as vomeronasal type 1 receptors (V1R) in dominance behavior by using a conditional knockout mouse for G protein subunit Gαi2, which is essential for V1R signaling. We used the tube test as a model to analyze the within-a-cage hierarchy in male mice, but also as a paradigm of novel territorial competition in animals from different cages. In absence of prior social experience, Gαi2 deletion promotes winning a novel social competition with an unfamiliar control mouse but had no effect on an established hierarchy in cages with mixed genotypes, both Gαi2−/− and controls. To further dissect social behavior of Gαi2−/− mice, we performed a 3-chamber sociability assay and found that mutants had a slightly altered social investigation. Finally, gene expression analysis in the medial prefrontal cortex (mPFC) for a subset of genes previously linked to social status revealed no differences between group-housed Gαi2−/− and controls. Our results reveal a direct influence of pheromone detection on territorial dominance, indicating that olfactory communication involving apical VNO receptors like V1R is important for the outcome of an initial social competition between two unfamiliar male mice, whereas final social status acquired within a cage remains unaffected. These results support the idea that previous social context is relevant for the development of social hierarchy of a group. Overall, our data identify two context-dependent forms of dominance, acute and chronic, and that pheromone signaling through V1R receptors is involved in the first stages of a social competition but in the long term is not predictive for high social ranks on a hierarchy.Fil: Pallé, Anna. Consejo Superior de Investigaciones Científicas; EspañaFil: Montero, Marta. Consejo Superior de Investigaciones Científicas; EspañaFil: Fernendez, Silvia. Consejo Superior de Investigaciones Científicas; EspañaFil: Tezanos, Patricia. Consejo Superior de Investigaciones Científicas; EspañaFil: De las Heras, Juan. Consejo Superior de Investigaciones Científicas; EspañaFil: Luskey, Valerie. Consejo Superior de Investigaciones Científicas; EspañaFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Zufall, Frank. Universitat Saarland; AlemaniaFil: Chamero, Pablo. Centre National de la Recherche Scientifique; FranciaFil: Trejo, José Luis. Consejo Superior de Investigaciones Científicas; EspañaNature2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141692Pallé, Anna; Montero, Marta; Fernendez, Silvia; Tezanos, Patricia; De las Heras, Juan; et al.; Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition; Nature; Scientific Reports; 10; 1; 12-2020; 1-122045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-57765-6info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-020-57765-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:40Zoai:ri.conicet.gov.ar:11336/141692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:40.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
title Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
spellingShingle Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
Pallé, Anna
Gαi2-/-
title_short Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
title_full Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
title_fullStr Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
title_full_unstemmed Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
title_sort Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition
dc.creator.none.fl_str_mv Pallé, Anna
Montero, Marta
Fernendez, Silvia
Tezanos, Patricia
De las Heras, Juan
Luskey, Valerie
Birnbaumer, Lutz
Zufall, Frank
Chamero, Pablo
Trejo, José Luis
author Pallé, Anna
author_facet Pallé, Anna
Montero, Marta
Fernendez, Silvia
Tezanos, Patricia
De las Heras, Juan
Luskey, Valerie
Birnbaumer, Lutz
Zufall, Frank
Chamero, Pablo
Trejo, José Luis
author_role author
author2 Montero, Marta
Fernendez, Silvia
Tezanos, Patricia
De las Heras, Juan
Luskey, Valerie
Birnbaumer, Lutz
Zufall, Frank
Chamero, Pablo
Trejo, José Luis
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gαi2-/-
topic Gαi2-/-
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pheromone detection by the vomeronasal organ (VNO) mediates important social behaviors across different species, including aggression and sexual behavior. However, the relationship between vomeronasal function and social hierarchy has not been analyzed reliably. We evaluated the role of pheromone detection by receptors expressed in the apical layer of the VNO such as vomeronasal type 1 receptors (V1R) in dominance behavior by using a conditional knockout mouse for G protein subunit Gαi2, which is essential for V1R signaling. We used the tube test as a model to analyze the within-a-cage hierarchy in male mice, but also as a paradigm of novel territorial competition in animals from different cages. In absence of prior social experience, Gαi2 deletion promotes winning a novel social competition with an unfamiliar control mouse but had no effect on an established hierarchy in cages with mixed genotypes, both Gαi2−/− and controls. To further dissect social behavior of Gαi2−/− mice, we performed a 3-chamber sociability assay and found that mutants had a slightly altered social investigation. Finally, gene expression analysis in the medial prefrontal cortex (mPFC) for a subset of genes previously linked to social status revealed no differences between group-housed Gαi2−/− and controls. Our results reveal a direct influence of pheromone detection on territorial dominance, indicating that olfactory communication involving apical VNO receptors like V1R is important for the outcome of an initial social competition between two unfamiliar male mice, whereas final social status acquired within a cage remains unaffected. These results support the idea that previous social context is relevant for the development of social hierarchy of a group. Overall, our data identify two context-dependent forms of dominance, acute and chronic, and that pheromone signaling through V1R receptors is involved in the first stages of a social competition but in the long term is not predictive for high social ranks on a hierarchy.
Fil: Pallé, Anna. Consejo Superior de Investigaciones Científicas; España
Fil: Montero, Marta. Consejo Superior de Investigaciones Científicas; España
Fil: Fernendez, Silvia. Consejo Superior de Investigaciones Científicas; España
Fil: Tezanos, Patricia. Consejo Superior de Investigaciones Científicas; España
Fil: De las Heras, Juan. Consejo Superior de Investigaciones Científicas; España
Fil: Luskey, Valerie. Consejo Superior de Investigaciones Científicas; España
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Zufall, Frank. Universitat Saarland; Alemania
Fil: Chamero, Pablo. Centre National de la Recherche Scientifique; Francia
Fil: Trejo, José Luis. Consejo Superior de Investigaciones Científicas; España
description Pheromone detection by the vomeronasal organ (VNO) mediates important social behaviors across different species, including aggression and sexual behavior. However, the relationship between vomeronasal function and social hierarchy has not been analyzed reliably. We evaluated the role of pheromone detection by receptors expressed in the apical layer of the VNO such as vomeronasal type 1 receptors (V1R) in dominance behavior by using a conditional knockout mouse for G protein subunit Gαi2, which is essential for V1R signaling. We used the tube test as a model to analyze the within-a-cage hierarchy in male mice, but also as a paradigm of novel territorial competition in animals from different cages. In absence of prior social experience, Gαi2 deletion promotes winning a novel social competition with an unfamiliar control mouse but had no effect on an established hierarchy in cages with mixed genotypes, both Gαi2−/− and controls. To further dissect social behavior of Gαi2−/− mice, we performed a 3-chamber sociability assay and found that mutants had a slightly altered social investigation. Finally, gene expression analysis in the medial prefrontal cortex (mPFC) for a subset of genes previously linked to social status revealed no differences between group-housed Gαi2−/− and controls. Our results reveal a direct influence of pheromone detection on territorial dominance, indicating that olfactory communication involving apical VNO receptors like V1R is important for the outcome of an initial social competition between two unfamiliar male mice, whereas final social status acquired within a cage remains unaffected. These results support the idea that previous social context is relevant for the development of social hierarchy of a group. Overall, our data identify two context-dependent forms of dominance, acute and chronic, and that pheromone signaling through V1R receptors is involved in the first stages of a social competition but in the long term is not predictive for high social ranks on a hierarchy.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141692
Pallé, Anna; Montero, Marta; Fernendez, Silvia; Tezanos, Patricia; De las Heras, Juan; et al.; Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition; Nature; Scientific Reports; 10; 1; 12-2020; 1-12
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141692
identifier_str_mv Pallé, Anna; Montero, Marta; Fernendez, Silvia; Tezanos, Patricia; De las Heras, Juan; et al.; Gαi2+ vomeronasal neurons govern the initial outcome of an acute social competition; Nature; Scientific Reports; 10; 1; 12-2020; 1-12
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-57765-6
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-020-57765-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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