Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis

Autores
Fontana, Ariel Ramón; Knuf, Franziska; Monasterio, Romina Paula; Schieber, Andreas
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The suitability of grape pomace and wine lees protein isolates as a source of bioactive peptides with antihypertensive activity was evaluated through hydrolysis with different proteolytic enzymes. Peptides were initially fractionated by ultrafiltration. The determination of the angiotensin-converting enzyme inhibitory activity evidenced that hydrolysates of Flavourzyme from grape pomace and of Alcalase from wine lees showed higher bioactivities. The fractions <3 kDa of these hydrolysates were further purified by semipreparative reversed-phase liquid chromatography. The peptidome of the fractions showing the highest angiotensin-converting enzyme inhibitory activities was characterised by nano-liquid chromatography-Orbitrap tandem mass spectrometry. The analysis of the chemical features of identified peptides like hydrophobicity and the frequency of angiotensin-converting enzyme inhibitory-active di-, tri- and tetrapeptide motives was associated with the antihypertensive activity. The peptides GPCKFYYGK, FSSFYYGK and YYGKF, among others, appear to contribute significantly to the antihypertensive activity of the hydrolysates.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Knuf, Franziska. Universitat Bonn; Alemania
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Schieber, Andreas. Universitat Bonn; Alemania
Materia
GRAPE POMACE
WINE LEES
PROTEIN HYDROLYSATES
BIOACTIVITY
ANGIOTENSIN I CONVERTING ENZYME INHIBITORY ACTIVITY
ORBITRAP TANDEM MASS SPECTROMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281793
Acceder
id CONICETDig_296fa4e4080ffd4e814bf6cbecdcfa47
oai_identifier_str oai:ri.conicet.gov.ar:11336/281793
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysisFontana, Ariel RamónKnuf, FranziskaMonasterio, Romina PaulaSchieber, AndreasGRAPE POMACEWINE LEESPROTEIN HYDROLYSATESBIOACTIVITYANGIOTENSIN I CONVERTING ENZYME INHIBITORY ACTIVITYORBITRAP TANDEM MASS SPECTROMETRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The suitability of grape pomace and wine lees protein isolates as a source of bioactive peptides with antihypertensive activity was evaluated through hydrolysis with different proteolytic enzymes. Peptides were initially fractionated by ultrafiltration. The determination of the angiotensin-converting enzyme inhibitory activity evidenced that hydrolysates of Flavourzyme from grape pomace and of Alcalase from wine lees showed higher bioactivities. The fractions <3 kDa of these hydrolysates were further purified by semipreparative reversed-phase liquid chromatography. The peptidome of the fractions showing the highest angiotensin-converting enzyme inhibitory activities was characterised by nano-liquid chromatography-Orbitrap tandem mass spectrometry. The analysis of the chemical features of identified peptides like hydrophobicity and the frequency of angiotensin-converting enzyme inhibitory-active di-, tri- and tetrapeptide motives was associated with the antihypertensive activity. The peptides GPCKFYYGK, FSSFYYGK and YYGKF, among others, appear to contribute significantly to the antihypertensive activity of the hydrolysates.Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Knuf, Franziska. Universitat Bonn; AlemaniaFil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Schieber, Andreas. Universitat Bonn; AlemaniaElsevier2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281793Fontana, Ariel Ramón; Knuf, Franziska; Monasterio, Romina Paula; Schieber, Andreas; Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis; Elsevier; Food Chemistry; 476; 2-2025; 1-120308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0308814625007290info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2025.143478info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-11T12:24:40Zoai:ri.conicet.gov.ar:11336/281793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-11 12:24:40.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
title Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
spellingShingle Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
Fontana, Ariel Ramón
GRAPE POMACE
WINE LEES
PROTEIN HYDROLYSATES
BIOACTIVITY
ANGIOTENSIN I CONVERTING ENZYME INHIBITORY ACTIVITY
ORBITRAP TANDEM MASS SPECTROMETRY
title_short Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
title_full Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
title_fullStr Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
title_full_unstemmed Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
title_sort Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis
dc.creator.none.fl_str_mv Fontana, Ariel Ramón
Knuf, Franziska
Monasterio, Romina Paula
Schieber, Andreas
author Fontana, Ariel Ramón
author_facet Fontana, Ariel Ramón
Knuf, Franziska
Monasterio, Romina Paula
Schieber, Andreas
author_role author
author2 Knuf, Franziska
Monasterio, Romina Paula
Schieber, Andreas
author2_role author
author
author
dc.subject.none.fl_str_mv GRAPE POMACE
WINE LEES
PROTEIN HYDROLYSATES
BIOACTIVITY
ANGIOTENSIN I CONVERTING ENZYME INHIBITORY ACTIVITY
ORBITRAP TANDEM MASS SPECTROMETRY
topic GRAPE POMACE
WINE LEES
PROTEIN HYDROLYSATES
BIOACTIVITY
ANGIOTENSIN I CONVERTING ENZYME INHIBITORY ACTIVITY
ORBITRAP TANDEM MASS SPECTROMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The suitability of grape pomace and wine lees protein isolates as a source of bioactive peptides with antihypertensive activity was evaluated through hydrolysis with different proteolytic enzymes. Peptides were initially fractionated by ultrafiltration. The determination of the angiotensin-converting enzyme inhibitory activity evidenced that hydrolysates of Flavourzyme from grape pomace and of Alcalase from wine lees showed higher bioactivities. The fractions <3 kDa of these hydrolysates were further purified by semipreparative reversed-phase liquid chromatography. The peptidome of the fractions showing the highest angiotensin-converting enzyme inhibitory activities was characterised by nano-liquid chromatography-Orbitrap tandem mass spectrometry. The analysis of the chemical features of identified peptides like hydrophobicity and the frequency of angiotensin-converting enzyme inhibitory-active di-, tri- and tetrapeptide motives was associated with the antihypertensive activity. The peptides GPCKFYYGK, FSSFYYGK and YYGKF, among others, appear to contribute significantly to the antihypertensive activity of the hydrolysates.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Knuf, Franziska. Universitat Bonn; Alemania
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Schieber, Andreas. Universitat Bonn; Alemania
description The suitability of grape pomace and wine lees protein isolates as a source of bioactive peptides with antihypertensive activity was evaluated through hydrolysis with different proteolytic enzymes. Peptides were initially fractionated by ultrafiltration. The determination of the angiotensin-converting enzyme inhibitory activity evidenced that hydrolysates of Flavourzyme from grape pomace and of Alcalase from wine lees showed higher bioactivities. The fractions <3 kDa of these hydrolysates were further purified by semipreparative reversed-phase liquid chromatography. The peptidome of the fractions showing the highest angiotensin-converting enzyme inhibitory activities was characterised by nano-liquid chromatography-Orbitrap tandem mass spectrometry. The analysis of the chemical features of identified peptides like hydrophobicity and the frequency of angiotensin-converting enzyme inhibitory-active di-, tri- and tetrapeptide motives was associated with the antihypertensive activity. The peptides GPCKFYYGK, FSSFYYGK and YYGKF, among others, appear to contribute significantly to the antihypertensive activity of the hydrolysates.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281793
Fontana, Ariel Ramón; Knuf, Franziska; Monasterio, Romina Paula; Schieber, Andreas; Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis; Elsevier; Food Chemistry; 476; 2-2025; 1-12
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281793
identifier_str_mv Fontana, Ariel Ramón; Knuf, Franziska; Monasterio, Romina Paula; Schieber, Andreas; Screening of wine industry by-products as a source of bioactive peptides: Fractionation, in vitro antihypertensive activity and peptidomics analysis; Elsevier; Food Chemistry; 476; 2-2025; 1-12
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0308814625007290
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2025.143478
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1859460390636748800
score 12.977003

Publicaciones similares