Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG

Autores
Sabín Fernández, Juan Daniel; Prieto, Gerardo; Ruso, Juan M.; Messina, Paula Verónica; Salgado, Francisco J.; Nogueira, Montserrat; Costas, Miguel; Sarmiento, Felix
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The interaction between two serum blood proteins, namely human serum albumin (HSA) and human immunoglobulin G (IgG), with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) liposomes has been studied in detail using dynamic light scattering, flow cytometry, enzyme-linked immunosorbent assay (ELISA), electrophoretic mobility, differential scanning calorimetry (DSC), and surface tension measurements. HSA and IgG interact with liposomes forming molecular aggregates that remain stable at protein concentrations beyond those of total liposome coverage. Both HSA and IgG penetrate into the liposome bilayer. An ELISA assay indicates that the Fc region of IgG is the one that is immersed in the DMPC membrane. The liposome−protein interaction is mainly of electrostatic nature, but an important hydrophobic contribution is also present.
Fil: Sabín Fernández, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salgado, Francisco J.. Universidad de Santiago de Compostela; España
Fil: Nogueira, Montserrat. Universidad de Santiago de Compostela. Facultad de Química; España
Fil: Costas, Miguel. Universidad Nacional Autonoma de Mexico. Facultad de Ciencias; México
Fil: Sarmiento, Felix. Universidad de Santiago de Compostela. Facultad de Física; España
Materia
Liposomes
Igg
Hsa
Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgGSabín Fernández, Juan DanielPrieto, GerardoRuso, Juan M.Messina, Paula VerónicaSalgado, Francisco J.Nogueira, MontserratCostas, MiguelSarmiento, FelixLiposomesIggHsaProteinshttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The interaction between two serum blood proteins, namely human serum albumin (HSA) and human immunoglobulin G (IgG), with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) liposomes has been studied in detail using dynamic light scattering, flow cytometry, enzyme-linked immunosorbent assay (ELISA), electrophoretic mobility, differential scanning calorimetry (DSC), and surface tension measurements. HSA and IgG interact with liposomes forming molecular aggregates that remain stable at protein concentrations beyond those of total liposome coverage. Both HSA and IgG penetrate into the liposome bilayer. An ELISA assay indicates that the Fc region of IgG is the one that is immersed in the DMPC membrane. The liposome−protein interaction is mainly of electrostatic nature, but an important hydrophobic contribution is also present.Fil: Sabín Fernández, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Salgado, Francisco J.. Universidad de Santiago de Compostela; EspañaFil: Nogueira, Montserrat. Universidad de Santiago de Compostela. Facultad de Química; EspañaFil: Costas, Miguel. Universidad Nacional Autonoma de Mexico. Facultad de Ciencias; MéxicoFil: Sarmiento, Felix. Universidad de Santiago de Compostela. Facultad de Física; EspañaAmerican Chemical Society2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66177Sabín Fernández, Juan Daniel; Prieto, Gerardo; Ruso, Juan M.; Messina, Paula Verónica; Salgado, Francisco J.; et al.; Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG; American Chemical Society; Journal of Physical Chemistry B; 113; 6; 2-2009; 1655-16611520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/jp804641einfo:eu-repo/semantics/altIdentifier/doi/10.1021/jp804641einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:43Zoai:ri.conicet.gov.ar:11336/66177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:43.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
title Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
spellingShingle Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
Sabín Fernández, Juan Daniel
Liposomes
Igg
Hsa
Proteins
title_short Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
title_full Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
title_fullStr Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
title_full_unstemmed Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
title_sort Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG
dc.creator.none.fl_str_mv Sabín Fernández, Juan Daniel
Prieto, Gerardo
Ruso, Juan M.
Messina, Paula Verónica
Salgado, Francisco J.
Nogueira, Montserrat
Costas, Miguel
Sarmiento, Felix
author Sabín Fernández, Juan Daniel
author_facet Sabín Fernández, Juan Daniel
Prieto, Gerardo
Ruso, Juan M.
Messina, Paula Verónica
Salgado, Francisco J.
Nogueira, Montserrat
Costas, Miguel
Sarmiento, Felix
author_role author
author2 Prieto, Gerardo
Ruso, Juan M.
Messina, Paula Verónica
Salgado, Francisco J.
Nogueira, Montserrat
Costas, Miguel
Sarmiento, Felix
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Liposomes
Igg
Hsa
Proteins
topic Liposomes
Igg
Hsa
Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The interaction between two serum blood proteins, namely human serum albumin (HSA) and human immunoglobulin G (IgG), with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) liposomes has been studied in detail using dynamic light scattering, flow cytometry, enzyme-linked immunosorbent assay (ELISA), electrophoretic mobility, differential scanning calorimetry (DSC), and surface tension measurements. HSA and IgG interact with liposomes forming molecular aggregates that remain stable at protein concentrations beyond those of total liposome coverage. Both HSA and IgG penetrate into the liposome bilayer. An ELISA assay indicates that the Fc region of IgG is the one that is immersed in the DMPC membrane. The liposome−protein interaction is mainly of electrostatic nature, but an important hydrophobic contribution is also present.
Fil: Sabín Fernández, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Prieto, Gerardo. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salgado, Francisco J.. Universidad de Santiago de Compostela; España
Fil: Nogueira, Montserrat. Universidad de Santiago de Compostela. Facultad de Química; España
Fil: Costas, Miguel. Universidad Nacional Autonoma de Mexico. Facultad de Ciencias; México
Fil: Sarmiento, Felix. Universidad de Santiago de Compostela. Facultad de Física; España
description The interaction between two serum blood proteins, namely human serum albumin (HSA) and human immunoglobulin G (IgG), with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) liposomes has been studied in detail using dynamic light scattering, flow cytometry, enzyme-linked immunosorbent assay (ELISA), electrophoretic mobility, differential scanning calorimetry (DSC), and surface tension measurements. HSA and IgG interact with liposomes forming molecular aggregates that remain stable at protein concentrations beyond those of total liposome coverage. Both HSA and IgG penetrate into the liposome bilayer. An ELISA assay indicates that the Fc region of IgG is the one that is immersed in the DMPC membrane. The liposome−protein interaction is mainly of electrostatic nature, but an important hydrophobic contribution is also present.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66177
Sabín Fernández, Juan Daniel; Prieto, Gerardo; Ruso, Juan M.; Messina, Paula Verónica; Salgado, Francisco J.; et al.; Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG; American Chemical Society; Journal of Physical Chemistry B; 113; 6; 2-2009; 1655-1661
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66177
identifier_str_mv Sabín Fernández, Juan Daniel; Prieto, Gerardo; Ruso, Juan M.; Messina, Paula Verónica; Salgado, Francisco J.; et al.; Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG; American Chemical Society; Journal of Physical Chemistry B; 113; 6; 2-2009; 1655-1661
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/jp804641e
info:eu-repo/semantics/altIdentifier/doi/10.1021/jp804641e
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269362520588288
score 13.13397

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