Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation

Autores
Sheridan, María Luján; Gomez, Maria Sol; Casati, Paula
Año de publicación
2020
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plants use sunlight to direct and regulate essential processes. Among the components of solar radiation is UV-B radiation (280-315 nm) that at high intensities generates harmful effects on plants. Because of this, plants have developed multiple mechanisms of tolerance and adaptation to UV-B radiation. Among the effects caused by high doses of UV-B radiation in plants are damage to DNA, lipids and proteins. Two common plant phenotypes after UV-B exposure are inhibition of leaf growth and primary root elongation.The shape and architecture of plants are determined by processes that modulate growth and differentiation of organs, which control the number, size and type of cells that constitute them. One of the pathways involved in the regulation of cell division, growth and differentiation is the Retinoblastoma pathway, in which the Retinoblastoma protein (RBR), the E2F transcription factors and the DP dimerization proteins participate. This pathway regulates the G1/S cell cycle transition, one of the key stages of cell cycle control in eukaryotes. E2F transcription factors serve crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In particular, E2Fa activates transcription binding to DNA cooperatively with DP proteins through a specific recognition site that is found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication.Based on this, the aim of this work is to understand the participation of E2F transcription factors, in particular E2Fa, in the response of Arabidopsis thaliana plants to ultraviolet-B radiation. In the laboratory, using mutant lines in the E2Fa gene (e2fa-1), we observed that the primary roots are less affected by a treatment with UV-B radiation than WT lines, when we analyzed their elongation and also at the cellular level. In addition, the primary roots of e2fa mutants showed significantly fewer dead meristematic cells after a UV-B treatment than WT plants. Regarding the aerial part of the plants, we also found that growth of the proliferating leaves of e2fa-1 lines is less affected by UV-B radiation than the WT leaves. Together, these results suggest that E2Fa regulates growth, development and programmed cell death in response to UV-B radiation.
Fil: Sheridan, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gomez, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
UV-B
Cell cycle
E2F
arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211382
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiationSheridan, María LujánGomez, Maria SolCasati, PaulaUV-BCell cycleE2Farabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plants use sunlight to direct and regulate essential processes. Among the components of solar radiation is UV-B radiation (280-315 nm) that at high intensities generates harmful effects on plants. Because of this, plants have developed multiple mechanisms of tolerance and adaptation to UV-B radiation. Among the effects caused by high doses of UV-B radiation in plants are damage to DNA, lipids and proteins. Two common plant phenotypes after UV-B exposure are inhibition of leaf growth and primary root elongation.The shape and architecture of plants are determined by processes that modulate growth and differentiation of organs, which control the number, size and type of cells that constitute them. One of the pathways involved in the regulation of cell division, growth and differentiation is the Retinoblastoma pathway, in which the Retinoblastoma protein (RBR), the E2F transcription factors and the DP dimerization proteins participate. This pathway regulates the G1/S cell cycle transition, one of the key stages of cell cycle control in eukaryotes. E2F transcription factors serve crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In particular, E2Fa activates transcription binding to DNA cooperatively with DP proteins through a specific recognition site that is found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication.Based on this, the aim of this work is to understand the participation of E2F transcription factors, in particular E2Fa, in the response of Arabidopsis thaliana plants to ultraviolet-B radiation. In the laboratory, using mutant lines in the E2Fa gene (e2fa-1), we observed that the primary roots are less affected by a treatment with UV-B radiation than WT lines, when we analyzed their elongation and also at the cellular level. In addition, the primary roots of e2fa mutants showed significantly fewer dead meristematic cells after a UV-B treatment than WT plants. Regarding the aerial part of the plants, we also found that growth of the proliferating leaves of e2fa-1 lines is less affected by UV-B radiation than the WT leaves. Together, these results suggest that E2Fa regulates growth, development and programmed cell death in response to UV-B radiation.Fil: Sheridan, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Gomez, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General MicrobiologyArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/211382Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 64-64CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:26Zoai:ri.conicet.gov.ar:11336/211382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:26.518CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
title Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
spellingShingle Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
Sheridan, María Luján
UV-B
Cell cycle
E2F
arabidopsis
title_short Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
title_full Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
title_fullStr Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
title_full_unstemmed Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
title_sort Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation
dc.creator.none.fl_str_mv Sheridan, María Luján
Gomez, Maria Sol
Casati, Paula
author Sheridan, María Luján
author_facet Sheridan, María Luján
Gomez, Maria Sol
Casati, Paula
author_role author
author2 Gomez, Maria Sol
Casati, Paula
author2_role author
author
dc.subject.none.fl_str_mv UV-B
Cell cycle
E2F
arabidopsis
topic UV-B
Cell cycle
E2F
arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants use sunlight to direct and regulate essential processes. Among the components of solar radiation is UV-B radiation (280-315 nm) that at high intensities generates harmful effects on plants. Because of this, plants have developed multiple mechanisms of tolerance and adaptation to UV-B radiation. Among the effects caused by high doses of UV-B radiation in plants are damage to DNA, lipids and proteins. Two common plant phenotypes after UV-B exposure are inhibition of leaf growth and primary root elongation.The shape and architecture of plants are determined by processes that modulate growth and differentiation of organs, which control the number, size and type of cells that constitute them. One of the pathways involved in the regulation of cell division, growth and differentiation is the Retinoblastoma pathway, in which the Retinoblastoma protein (RBR), the E2F transcription factors and the DP dimerization proteins participate. This pathway regulates the G1/S cell cycle transition, one of the key stages of cell cycle control in eukaryotes. E2F transcription factors serve crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In particular, E2Fa activates transcription binding to DNA cooperatively with DP proteins through a specific recognition site that is found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication.Based on this, the aim of this work is to understand the participation of E2F transcription factors, in particular E2Fa, in the response of Arabidopsis thaliana plants to ultraviolet-B radiation. In the laboratory, using mutant lines in the E2Fa gene (e2fa-1), we observed that the primary roots are less affected by a treatment with UV-B radiation than WT lines, when we analyzed their elongation and also at the cellular level. In addition, the primary roots of e2fa mutants showed significantly fewer dead meristematic cells after a UV-B treatment than WT plants. Regarding the aerial part of the plants, we also found that growth of the proliferating leaves of e2fa-1 lines is less affected by UV-B radiation than the WT leaves. Together, these results suggest that E2Fa regulates growth, development and programmed cell death in response to UV-B radiation.
Fil: Sheridan, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gomez, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Plants use sunlight to direct and regulate essential processes. Among the components of solar radiation is UV-B radiation (280-315 nm) that at high intensities generates harmful effects on plants. Because of this, plants have developed multiple mechanisms of tolerance and adaptation to UV-B radiation. Among the effects caused by high doses of UV-B radiation in plants are damage to DNA, lipids and proteins. Two common plant phenotypes after UV-B exposure are inhibition of leaf growth and primary root elongation.The shape and architecture of plants are determined by processes that modulate growth and differentiation of organs, which control the number, size and type of cells that constitute them. One of the pathways involved in the regulation of cell division, growth and differentiation is the Retinoblastoma pathway, in which the Retinoblastoma protein (RBR), the E2F transcription factors and the DP dimerization proteins participate. This pathway regulates the G1/S cell cycle transition, one of the key stages of cell cycle control in eukaryotes. E2F transcription factors serve crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In particular, E2Fa activates transcription binding to DNA cooperatively with DP proteins through a specific recognition site that is found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication.Based on this, the aim of this work is to understand the participation of E2F transcription factors, in particular E2Fa, in the response of Arabidopsis thaliana plants to ultraviolet-B radiation. In the laboratory, using mutant lines in the E2Fa gene (e2fa-1), we observed that the primary roots are less affected by a treatment with UV-B radiation than WT lines, when we analyzed their elongation and also at the cellular level. In addition, the primary roots of e2fa mutants showed significantly fewer dead meristematic cells after a UV-B treatment than WT plants. Regarding the aerial part of the plants, we also found that growth of the proliferating leaves of e2fa-1 lines is less affected by UV-B radiation than the WT leaves. Together, these results suggest that E2Fa regulates growth, development and programmed cell death in response to UV-B radiation.
publishDate 2020
dc.date.none.fl_str_mv 2020
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info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211382
Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 64-64
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211382
identifier_str_mv Analysis of E2FA protein in the response of arabidopsis thaliana plants to UV-B radiation; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 64-64
CONICET Digital
CONICET
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