Contribution of LIMYB in arabidopsis thaliana UV-B response
- Autores
- Petrich, Julieta; Ramos, Rocío Soledad; Casati, Paula; Spampinato, Claudia Patricia; Falcone Ferreyra, María Lorena
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.
Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Bioquímica e Investigación en Biología Molecular
Sociedad Argentina de Microbiología General - Materia
-
UV-B response
Arabidopsis
Translation
LIMYB - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/204461
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Contribution of LIMYB in arabidopsis thaliana UV-B responsePetrich, JulietaRamos, Rocío SoledadCasati, PaulaSpampinato, Claudia PatriciaFalcone Ferreyra, María LorenaUV-B responseArabidopsisTranslationLIMYBhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General MicrobiologyCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Bioquímica e Investigación en Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/204461Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-1600327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/TSP_BIOCELL_46213-SAIB-SAMIGE%202021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:52Zoai:ri.conicet.gov.ar:11336/204461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:52.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
title |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
spellingShingle |
Contribution of LIMYB in arabidopsis thaliana UV-B response Petrich, Julieta UV-B response Arabidopsis Translation LIMYB |
title_short |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
title_full |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
title_fullStr |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
title_full_unstemmed |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
title_sort |
Contribution of LIMYB in arabidopsis thaliana UV-B response |
dc.creator.none.fl_str_mv |
Petrich, Julieta Ramos, Rocío Soledad Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena |
author |
Petrich, Julieta |
author_facet |
Petrich, Julieta Ramos, Rocío Soledad Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena |
author_role |
author |
author2 |
Ramos, Rocío Soledad Casati, Paula Spampinato, Claudia Patricia Falcone Ferreyra, María Lorena |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
UV-B response Arabidopsis Translation LIMYB |
topic |
UV-B response Arabidopsis Translation LIMYB |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis. Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology Ciudad Autónoma de Buenos Aires Argentina Sociedad Argentina de Bioquímica e Investigación en Biología Molecular Sociedad Argentina de Microbiología General |
description |
LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/204461 Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-160 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/204461 |
identifier_str_mv |
Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-160 0327-9545 CONICET Digital CONICET |
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eng |
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eng |
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Internacional |
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Tech Science Press |
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Tech Science Press |
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