Contribution of LIMYB in arabidopsis thaliana UV-B response

Autores
Petrich, Julieta; Ramos, Rocío Soledad; Casati, Paula; Spampinato, Claudia Patricia; Falcone Ferreyra, María Lorena
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.
Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Bioquímica e Investigación en Biología Molecular
Sociedad Argentina de Microbiología General
Materia
UV-B response
Arabidopsis
Translation
LIMYB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/204461
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/204461
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Contribution of LIMYB in arabidopsis thaliana UV-B responsePetrich, JulietaRamos, Rocío SoledadCasati, PaulaSpampinato, Claudia PatriciaFalcone Ferreyra, María LorenaUV-B responseArabidopsisTranslationLIMYBhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General MicrobiologyCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Bioquímica e Investigación en Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/204461Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-1600327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/TSP_BIOCELL_46213-SAIB-SAMIGE%202021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:56:38Zoai:ri.conicet.gov.ar:11336/204461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:56:38.855CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Contribution of LIMYB in arabidopsis thaliana UV-B response
title Contribution of LIMYB in arabidopsis thaliana UV-B response
spellingShingle Contribution of LIMYB in arabidopsis thaliana UV-B response
Petrich, Julieta
UV-B response
Arabidopsis
Translation
LIMYB
title_short Contribution of LIMYB in arabidopsis thaliana UV-B response
title_full Contribution of LIMYB in arabidopsis thaliana UV-B response
title_fullStr Contribution of LIMYB in arabidopsis thaliana UV-B response
title_full_unstemmed Contribution of LIMYB in arabidopsis thaliana UV-B response
title_sort Contribution of LIMYB in arabidopsis thaliana UV-B response
dc.creator.none.fl_str_mv Petrich, Julieta
Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia Patricia
Falcone Ferreyra, María Lorena
author Petrich, Julieta
author_facet Petrich, Julieta
Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia Patricia
Falcone Ferreyra, María Lorena
author_role author
author2 Ramos, Rocío Soledad
Casati, Paula
Spampinato, Claudia Patricia
Falcone Ferreyra, María Lorena
author2_role author
author
author
author
dc.subject.none.fl_str_mv UV-B response
Arabidopsis
Translation
LIMYB
topic UV-B response
Arabidopsis
Translation
LIMYB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.
Fil: Petrich, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Ramos, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Bioquímica e Investigación en Biología Molecular
Sociedad Argentina de Microbiología General
description LIMYB is a transcription factor involved in Arabidopsis thaliana plant defense against viral infection. In this signaling pathway, LIMYB interacts with the ribosomal protein RPL10A in nuclei to downregulate translational machinery. Thus, A. thaliana plants overexpressing LIMYB show lower protein synthesis, less association of viral mRNA with polisomes and higher tolerance to viral infection. Although LIMYB expression is not regulated by UV-B radiation, our aim is to elucidate whether LIMYB, in cooperation or not with RPL10A, is involved in UV-B response. For this purpose, we investigated the effect of UV-B in Arabidopsis limyb mutants and overexpressing plants. First, we analyzed transcript levels of previously described LIMYB target genes such as RPL4, RPL13, RPS25, RPL28e and RPS13a after UV-B exposure. Both the mutation and the overexpression of LIMYB mainly does not affect the regulation of gene expression by UV-B radiation. However, LIMYB-overexpressing lines show less inhibition of primary root elongation after UV-B treatment with respect to WT plants, while the opposite is observed in limyb mutants. In addition, LIMYB-overexpressing plants exhibit decreased membrane damage, not change in the chlorophyll content and increased levels of UV-absorbing phenolic compounds after UV-B exposure in comparison to WT and mutant plants. Our results suggest that LIMYB might participate in UV-B response and its overexpression could improve UV-B tolerance in Arabidopsis.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/204461
Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-160
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/204461
identifier_str_mv Contribution of LIMYB in arabidopsis thaliana UV-B response; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 159-160
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/TSP_BIOCELL_46213-SAIB-SAMIGE%202021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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