Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans

Autores
Dudiuk, Catiana Beatriz; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia; Macedo, Daiana; Perlin, David S.; Garcia, Guillermo Manuel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Santa Fe. Unidad de Adm.territorial; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Jimenez Ortigoza, Cristina. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados Unidos
Fil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Perlin, David S.. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados Unidos
Fil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Candida
Albicans
Echinocandins
Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19408
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicansDudiuk, Catiana BeatrizGamarra, SoledadJimenez Ortigoza, CristinaLeonardelli, FlorenciaMacedo, DaianaPerlin, David S.Garcia, Guillermo ManuelCandidaAlbicansEchinocandinsResistancehttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Santa Fe. Unidad de Adm.territorial; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Jimenez Ortigoza, Cristina. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados UnidosFil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Perlin, David S.. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados UnidosFil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaAmerican Society for Microbiology2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19408Dudiuk, Catiana Beatriz; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia; Macedo, Daiana; et al.; Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans; American Society for Microbiology; Journal of Clinical Microbiology; 53; 7; 7-2015; 2037-20410095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.00398-15info:eu-repo/semantics/altIdentifier/url/http://jcm.asm.org/content/53/7/2037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:30:14Zoai:ri.conicet.gov.ar:11336/19408instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:30:14.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
title Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
spellingShingle Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
Dudiuk, Catiana Beatriz
Candida
Albicans
Echinocandins
Resistance
title_short Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
title_full Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
title_fullStr Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
title_full_unstemmed Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
title_sort Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans
dc.creator.none.fl_str_mv Dudiuk, Catiana Beatriz
Gamarra, Soledad
Jimenez Ortigoza, Cristina
Leonardelli, Florencia
Macedo, Daiana
Perlin, David S.
Garcia, Guillermo Manuel
author Dudiuk, Catiana Beatriz
author_facet Dudiuk, Catiana Beatriz
Gamarra, Soledad
Jimenez Ortigoza, Cristina
Leonardelli, Florencia
Macedo, Daiana
Perlin, David S.
Garcia, Guillermo Manuel
author_role author
author2 Gamarra, Soledad
Jimenez Ortigoza, Cristina
Leonardelli, Florencia
Macedo, Daiana
Perlin, David S.
Garcia, Guillermo Manuel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Candida
Albicans
Echinocandins
Resistance
topic Candida
Albicans
Echinocandins
Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Santa Fe. Unidad de Adm.territorial; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Jimenez Ortigoza, Cristina. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados Unidos
Fil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Perlin, David S.. Public Health Research Institute. Rutgers Biomedical and Health Science; Estados Unidos
Fil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19408
Dudiuk, Catiana Beatriz; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia; Macedo, Daiana; et al.; Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans; American Society for Microbiology; Journal of Clinical Microbiology; 53; 7; 7-2015; 2037-2041
0095-1137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19408
identifier_str_mv Dudiuk, Catiana Beatriz; Gamarra, Soledad; Jimenez Ortigoza, Cristina; Leonardelli, Florencia; Macedo, Daiana; et al.; Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans; American Society for Microbiology; Journal of Clinical Microbiology; 53; 7; 7-2015; 2037-2041
0095-1137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.00398-15
info:eu-repo/semantics/altIdentifier/url/http://jcm.asm.org/content/53/7/2037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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