RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis

Autores
Colo, Georgina Pamela; Rosato, Roberto R.; Grant, Steven; Costas, Monica Alejandra
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The nuclear receptor coactivator RAC3 plays important roles in many biological processes and tumorigenesis. We found that RAC3 is over-expressed in human chronic myeloid leukemia cells K562, which are normally resistant to TRAIL-induced apoptosis. RAC3 down-regulation by siRNA rendered these cells sensitive to TRAIL-induced cell death. In addition to the up-regulation of TRAIL receptors, the process involves Bid, caspases and PARP activation, loss of mitochondrial membrane potential, and release of AIF, cytochrome c and Smac/DIABLO to the cytoplasm. We conclude that RAC3 is required for TRAIL resistance and that this anti-apoptotic function is independent of its role in hormone receptor signaling.
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rosato, Roberto R.. Virginia Commonwealth University; Estados Unidos
Fil: Grant, Steven. Virginia Commonwealth University; Estados Unidos
Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Materia
Nuclear receptors coactivator
RAC3
Apoptosis
Leukemia
TRAIL
NF-kappaB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105931
Acceder
id CONICETDig_2892d8df56f95338241652956a231d1e
oai_identifier_str oai:ri.conicet.gov.ar:11336/105931
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosisColo, Georgina PamelaRosato, Roberto R.Grant, StevenCostas, Monica AlejandraNuclear receptors coactivatorRAC3ApoptosisLeukemiaTRAILNF-kappaBhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The nuclear receptor coactivator RAC3 plays important roles in many biological processes and tumorigenesis. We found that RAC3 is over-expressed in human chronic myeloid leukemia cells K562, which are normally resistant to TRAIL-induced apoptosis. RAC3 down-regulation by siRNA rendered these cells sensitive to TRAIL-induced cell death. In addition to the up-regulation of TRAIL receptors, the process involves Bid, caspases and PARP activation, loss of mitochondrial membrane potential, and release of AIF, cytochrome c and Smac/DIABLO to the cytoplasm. We conclude that RAC3 is required for TRAIL resistance and that this anti-apoptotic function is independent of its role in hormone receptor signaling.Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Rosato, Roberto R.. Virginia Commonwealth University; Estados UnidosFil: Grant, Steven. Virginia Commonwealth University; Estados UnidosFil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaElsevier Science2007-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105931Colo, Georgina Pamela; Rosato, Roberto R.; Grant, Steven; Costas, Monica Alejandra; RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis; Elsevier Science; FEBS Letters; 581; 26; 10-2007; 5075-50810014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2007.09.052info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2007.09.052info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:35Zoai:ri.conicet.gov.ar:11336/105931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:36.155CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
title RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
spellingShingle RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
Colo, Georgina Pamela
Nuclear receptors coactivator
RAC3
Apoptosis
Leukemia
TRAIL
NF-kappaB
title_short RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
title_full RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
title_fullStr RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
title_full_unstemmed RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
title_sort RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis
dc.creator.none.fl_str_mv Colo, Georgina Pamela
Rosato, Roberto R.
Grant, Steven
Costas, Monica Alejandra
author Colo, Georgina Pamela
author_facet Colo, Georgina Pamela
Rosato, Roberto R.
Grant, Steven
Costas, Monica Alejandra
author_role author
author2 Rosato, Roberto R.
Grant, Steven
Costas, Monica Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Nuclear receptors coactivator
RAC3
Apoptosis
Leukemia
TRAIL
NF-kappaB
topic Nuclear receptors coactivator
RAC3
Apoptosis
Leukemia
TRAIL
NF-kappaB
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The nuclear receptor coactivator RAC3 plays important roles in many biological processes and tumorigenesis. We found that RAC3 is over-expressed in human chronic myeloid leukemia cells K562, which are normally resistant to TRAIL-induced apoptosis. RAC3 down-regulation by siRNA rendered these cells sensitive to TRAIL-induced cell death. In addition to the up-regulation of TRAIL receptors, the process involves Bid, caspases and PARP activation, loss of mitochondrial membrane potential, and release of AIF, cytochrome c and Smac/DIABLO to the cytoplasm. We conclude that RAC3 is required for TRAIL resistance and that this anti-apoptotic function is independent of its role in hormone receptor signaling.
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rosato, Roberto R.. Virginia Commonwealth University; Estados Unidos
Fil: Grant, Steven. Virginia Commonwealth University; Estados Unidos
Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
description The nuclear receptor coactivator RAC3 plays important roles in many biological processes and tumorigenesis. We found that RAC3 is over-expressed in human chronic myeloid leukemia cells K562, which are normally resistant to TRAIL-induced apoptosis. RAC3 down-regulation by siRNA rendered these cells sensitive to TRAIL-induced cell death. In addition to the up-regulation of TRAIL receptors, the process involves Bid, caspases and PARP activation, loss of mitochondrial membrane potential, and release of AIF, cytochrome c and Smac/DIABLO to the cytoplasm. We conclude that RAC3 is required for TRAIL resistance and that this anti-apoptotic function is independent of its role in hormone receptor signaling.
publishDate 2007
dc.date.none.fl_str_mv 2007-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105931
Colo, Georgina Pamela; Rosato, Roberto R.; Grant, Steven; Costas, Monica Alejandra; RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis; Elsevier Science; FEBS Letters; 581; 26; 10-2007; 5075-5081
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105931
identifier_str_mv Colo, Georgina Pamela; Rosato, Roberto R.; Grant, Steven; Costas, Monica Alejandra; RAC3 down-regulation sensitizes human chronic myeloid leukemia cells to TRAIL-induced apoptosis; Elsevier Science; FEBS Letters; 581; 26; 10-2007; 5075-5081
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2007.09.052
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2007.09.052
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613070636187648
score 13.070432

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