Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells

Autores
Méndez, Fernando; Romero, Nicolás; Cubas, Liliana L.; Delgui, Laura Ruth; Rodriguez, Dolores; Rodríguez, José F.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
nfectious bursal disease virus (IBDV), a member of the Birnaviridae family, is responsible for a devastating immunosuppressive disease affecting juvenile domestic chickens. IBDV particles are naked icosahedrons enclosing a bipartite double-stranded RNA genome harboring three open reading frames (ORF). One of these ORFs codes for VP5, a non-structural polypeptide dispensable for virus replication in tissue culture but essential for IBDV pathogenesis. Using two previously described recombinant viruses, whose genomes differ in a single nucleotide, expressing or not the VP5 polypeptide, we have analyzed the role of this polypeptide during the IBDV replication process. Here, we show that VP5 is not involved in house-keeping steps of the virus replication cycle; i.e. genome transcription/replication, protein translation and virus assembly. Although infection with the VP5 expressing and non-expressing viruses rendered similar intracellular infective progeny yields, striking differences were detected on the ability of their progenies to exiting infected cells. Experimental data shows that the bulk of the VP5-expressing virus progeny efficiently egresses infected cells during the early phase of the infection, when viral metabolism is peaking and virus-induced cell death rates are as yet minimal, as determined by qPCR, radioactive protein labeling and quantitative real-time cell death analyses. In contrast, the release of the VP5-deficient virus progeny is significantly abridged and associated to cell death. Taken together, data presented in this report show that IBDV uses a previously undescribed VP5-dependent non-lytic egress mechanism significantly enhancing the virus dissemination speed. Ultrastructural analyses revealed that newly assembled IBDV virions associate to a vesicular network apparently facilitating their trafficking from virus assembly factories to the extracellular milieu, and that this association requires the expression of the VP5 polypeptide.
Fil: Méndez, Fernando. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Romero, Nicolás. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Cubas, Liliana L.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Rodriguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Rodríguez, José F.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Materia
IBDV,
BIRNAVIRUS
VIRUS EGRESS
VIRUS DISSEMINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48922
Acceder
id CONICETDig_2827f0db72847aa761fa364f2c329e19
oai_identifier_str oai:ri.conicet.gov.ar:11336/48922
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cellsMéndez, FernandoRomero, NicolásCubas, Liliana L.Delgui, Laura RuthRodriguez, DoloresRodríguez, José F.IBDV,BIRNAVIRUSVIRUS EGRESSVIRUS DISSEMINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1nfectious bursal disease virus (IBDV), a member of the Birnaviridae family, is responsible for a devastating immunosuppressive disease affecting juvenile domestic chickens. IBDV particles are naked icosahedrons enclosing a bipartite double-stranded RNA genome harboring three open reading frames (ORF). One of these ORFs codes for VP5, a non-structural polypeptide dispensable for virus replication in tissue culture but essential for IBDV pathogenesis. Using two previously described recombinant viruses, whose genomes differ in a single nucleotide, expressing or not the VP5 polypeptide, we have analyzed the role of this polypeptide during the IBDV replication process. Here, we show that VP5 is not involved in house-keeping steps of the virus replication cycle; i.e. genome transcription/replication, protein translation and virus assembly. Although infection with the VP5 expressing and non-expressing viruses rendered similar intracellular infective progeny yields, striking differences were detected on the ability of their progenies to exiting infected cells. Experimental data shows that the bulk of the VP5-expressing virus progeny efficiently egresses infected cells during the early phase of the infection, when viral metabolism is peaking and virus-induced cell death rates are as yet minimal, as determined by qPCR, radioactive protein labeling and quantitative real-time cell death analyses. In contrast, the release of the VP5-deficient virus progeny is significantly abridged and associated to cell death. Taken together, data presented in this report show that IBDV uses a previously undescribed VP5-dependent non-lytic egress mechanism significantly enhancing the virus dissemination speed. Ultrastructural analyses revealed that newly assembled IBDV virions associate to a vesicular network apparently facilitating their trafficking from virus assembly factories to the extracellular milieu, and that this association requires the expression of the VP5 polypeptide.Fil: Méndez, Fernando. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Romero, Nicolás. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Cubas, Liliana L.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Rodriguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Rodríguez, José F.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaPublic Library of Science2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48922Méndez, Fernando; Romero, Nicolás; Cubas, Liliana L.; Delgui, Laura Ruth; Rodriguez, Dolores; et al.; Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells; Public Library of Science; Plos One; 12; 1; 1-2017; 1-221932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0170080info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170080info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:05:28Zoai:ri.conicet.gov.ar:11336/48922instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:05:28.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
title Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
spellingShingle Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
Méndez, Fernando
IBDV,
BIRNAVIRUS
VIRUS EGRESS
VIRUS DISSEMINATION
title_short Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
title_full Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
title_fullStr Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
title_full_unstemmed Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
title_sort Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells
dc.creator.none.fl_str_mv Méndez, Fernando
Romero, Nicolás
Cubas, Liliana L.
Delgui, Laura Ruth
Rodriguez, Dolores
Rodríguez, José F.
author Méndez, Fernando
author_facet Méndez, Fernando
Romero, Nicolás
Cubas, Liliana L.
Delgui, Laura Ruth
Rodriguez, Dolores
Rodríguez, José F.
author_role author
author2 Romero, Nicolás
Cubas, Liliana L.
Delgui, Laura Ruth
Rodriguez, Dolores
Rodríguez, José F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv IBDV,
BIRNAVIRUS
VIRUS EGRESS
VIRUS DISSEMINATION
topic IBDV,
BIRNAVIRUS
VIRUS EGRESS
VIRUS DISSEMINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv nfectious bursal disease virus (IBDV), a member of the Birnaviridae family, is responsible for a devastating immunosuppressive disease affecting juvenile domestic chickens. IBDV particles are naked icosahedrons enclosing a bipartite double-stranded RNA genome harboring three open reading frames (ORF). One of these ORFs codes for VP5, a non-structural polypeptide dispensable for virus replication in tissue culture but essential for IBDV pathogenesis. Using two previously described recombinant viruses, whose genomes differ in a single nucleotide, expressing or not the VP5 polypeptide, we have analyzed the role of this polypeptide during the IBDV replication process. Here, we show that VP5 is not involved in house-keeping steps of the virus replication cycle; i.e. genome transcription/replication, protein translation and virus assembly. Although infection with the VP5 expressing and non-expressing viruses rendered similar intracellular infective progeny yields, striking differences were detected on the ability of their progenies to exiting infected cells. Experimental data shows that the bulk of the VP5-expressing virus progeny efficiently egresses infected cells during the early phase of the infection, when viral metabolism is peaking and virus-induced cell death rates are as yet minimal, as determined by qPCR, radioactive protein labeling and quantitative real-time cell death analyses. In contrast, the release of the VP5-deficient virus progeny is significantly abridged and associated to cell death. Taken together, data presented in this report show that IBDV uses a previously undescribed VP5-dependent non-lytic egress mechanism significantly enhancing the virus dissemination speed. Ultrastructural analyses revealed that newly assembled IBDV virions associate to a vesicular network apparently facilitating their trafficking from virus assembly factories to the extracellular milieu, and that this association requires the expression of the VP5 polypeptide.
Fil: Méndez, Fernando. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Romero, Nicolás. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Cubas, Liliana L.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Rodriguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Rodríguez, José F.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
description nfectious bursal disease virus (IBDV), a member of the Birnaviridae family, is responsible for a devastating immunosuppressive disease affecting juvenile domestic chickens. IBDV particles are naked icosahedrons enclosing a bipartite double-stranded RNA genome harboring three open reading frames (ORF). One of these ORFs codes for VP5, a non-structural polypeptide dispensable for virus replication in tissue culture but essential for IBDV pathogenesis. Using two previously described recombinant viruses, whose genomes differ in a single nucleotide, expressing or not the VP5 polypeptide, we have analyzed the role of this polypeptide during the IBDV replication process. Here, we show that VP5 is not involved in house-keeping steps of the virus replication cycle; i.e. genome transcription/replication, protein translation and virus assembly. Although infection with the VP5 expressing and non-expressing viruses rendered similar intracellular infective progeny yields, striking differences were detected on the ability of their progenies to exiting infected cells. Experimental data shows that the bulk of the VP5-expressing virus progeny efficiently egresses infected cells during the early phase of the infection, when viral metabolism is peaking and virus-induced cell death rates are as yet minimal, as determined by qPCR, radioactive protein labeling and quantitative real-time cell death analyses. In contrast, the release of the VP5-deficient virus progeny is significantly abridged and associated to cell death. Taken together, data presented in this report show that IBDV uses a previously undescribed VP5-dependent non-lytic egress mechanism significantly enhancing the virus dissemination speed. Ultrastructural analyses revealed that newly assembled IBDV virions associate to a vesicular network apparently facilitating their trafficking from virus assembly factories to the extracellular milieu, and that this association requires the expression of the VP5 polypeptide.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48922
Méndez, Fernando; Romero, Nicolás; Cubas, Liliana L.; Delgui, Laura Ruth; Rodriguez, Dolores; et al.; Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells; Public Library of Science; Plos One; 12; 1; 1-2017; 1-22
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48922
identifier_str_mv Méndez, Fernando; Romero, Nicolás; Cubas, Liliana L.; Delgui, Laura Ruth; Rodriguez, Dolores; et al.; Non-lytic egression of infectious bursal disease virus (IBDV) particles from infected cells; Public Library of Science; Plos One; 12; 1; 1-2017; 1-22
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0170080
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170080
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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