Thin sections techniques in fossil remains of mammals impregnated with asphalt

Autores
Garrone, Mariana Carolina; Ortiz, Herácleo O.; Prado, José L.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Paleohistological studies of vertebrates are based on the analysis and interpretation of bone microstructure of petrographic thin sections. These sections are mainly performed following standard techniques; however, the methodology can vary according to the particular characteristics of the sample (e.g. skeletal element, type of preservation) or the purpose of the work. As part of a paleohistological study on Equus occidentalis recovered from a Late Pleistocene asphalt seep ("tar pit"), Rancho La Brea (California, United States), in this contribution we describe the technique used to make thin sections of fossils impregnated with asphalt. The hydrocarbons act as a release agent, which causes the resins commonly used for glued or embedding not to adhere to the fossil remains. For chip preparation (=block) the sample were embedded in a large volume of low-viscosity epoxy resin, which generated a higher shrinkage and prevented the separation of the chip components (resin / fossil). The chip must be mounted on the glass slide using UV acrylic resin, which, unlike epoxy resins, does not require heat application, optimizes adhesion, and reduces the curing time, offering the possibility of cutting and polishing quickly after mounting. During polishing, it was necessary to reduce the cleaning time with ultrasound to avoid peeling and loss of portions of the sample. Based on the results obtained, we consider that this variant of the traditional methodology is optimal to work with fossils preserved in this particular context, as it allows making complete thin sections without altering the original osteohistological features.
Fil: Garrone, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; Argentina
Fil: Ortiz, Herácleo O.. Universidad Nacional del Sur. Departamento de Geología; Argentina
Fil: Prado, José L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; Argentina
2nd Palaeontological Virtual Congress
España
Palaeontological Virtual Congress
Materia
PALEOHISTOLOGY
VERTEBRATES
TECHNIQUES
THIN SECTIONS
ASPHALT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/175774

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spelling Thin sections techniques in fossil remains of mammals impregnated with asphaltGarrone, Mariana CarolinaOrtiz, Herácleo O.Prado, José L.PALEOHISTOLOGYVERTEBRATESTECHNIQUESTHIN SECTIONSASPHALThttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Paleohistological studies of vertebrates are based on the analysis and interpretation of bone microstructure of petrographic thin sections. These sections are mainly performed following standard techniques; however, the methodology can vary according to the particular characteristics of the sample (e.g. skeletal element, type of preservation) or the purpose of the work. As part of a paleohistological study on Equus occidentalis recovered from a Late Pleistocene asphalt seep ("tar pit"), Rancho La Brea (California, United States), in this contribution we describe the technique used to make thin sections of fossils impregnated with asphalt. The hydrocarbons act as a release agent, which causes the resins commonly used for glued or embedding not to adhere to the fossil remains. For chip preparation (=block) the sample were embedded in a large volume of low-viscosity epoxy resin, which generated a higher shrinkage and prevented the separation of the chip components (resin / fossil). The chip must be mounted on the glass slide using UV acrylic resin, which, unlike epoxy resins, does not require heat application, optimizes adhesion, and reduces the curing time, offering the possibility of cutting and polishing quickly after mounting. During polishing, it was necessary to reduce the cleaning time with ultrasound to avoid peeling and loss of portions of the sample. Based on the results obtained, we consider that this variant of the traditional methodology is optimal to work with fossils preserved in this particular context, as it allows making complete thin sections without altering the original osteohistological features.Fil: Garrone, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Ortiz, Herácleo O.. Universidad Nacional del Sur. Departamento de Geología; ArgentinaFil: Prado, José L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; Argentina2nd Palaeontological Virtual CongressEspañaPalaeontological Virtual CongressPalaeontological Virtual CongressVlachos, EvangelosManzanares, EstherCrespo, Vicente D.Martinez Perez, CarlosFerrón, Humberto G.Herráiz, José LuisGamonal González Iglesias, ArturoArnal, Fernando Antonio M.Gascó, FrancescCitton, Paolo2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/175774Thin sections techniques in fossil remains of mammals impregnated with asphalt; 2nd Palaeontological Virtual Congress; España; 2020; 95-95978-84-09-20283-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://palaeovc.org/index.php/downloads/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:27Zoai:ri.conicet.gov.ar:11336/175774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:28.373CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thin sections techniques in fossil remains of mammals impregnated with asphalt
title Thin sections techniques in fossil remains of mammals impregnated with asphalt
spellingShingle Thin sections techniques in fossil remains of mammals impregnated with asphalt
Garrone, Mariana Carolina
PALEOHISTOLOGY
VERTEBRATES
TECHNIQUES
THIN SECTIONS
ASPHALT
title_short Thin sections techniques in fossil remains of mammals impregnated with asphalt
title_full Thin sections techniques in fossil remains of mammals impregnated with asphalt
title_fullStr Thin sections techniques in fossil remains of mammals impregnated with asphalt
title_full_unstemmed Thin sections techniques in fossil remains of mammals impregnated with asphalt
title_sort Thin sections techniques in fossil remains of mammals impregnated with asphalt
dc.creator.none.fl_str_mv Garrone, Mariana Carolina
Ortiz, Herácleo O.
Prado, José L.
author Garrone, Mariana Carolina
author_facet Garrone, Mariana Carolina
Ortiz, Herácleo O.
Prado, José L.
author_role author
author2 Ortiz, Herácleo O.
Prado, José L.
author2_role author
author
dc.contributor.none.fl_str_mv Vlachos, Evangelos
Manzanares, Esther
Crespo, Vicente D.
Martinez Perez, Carlos
Ferrón, Humberto G.
Herráiz, José Luis
Gamonal González Iglesias, Arturo
Arnal, Fernando Antonio M.
Gascó, Francesc
Citton, Paolo
dc.subject.none.fl_str_mv PALEOHISTOLOGY
VERTEBRATES
TECHNIQUES
THIN SECTIONS
ASPHALT
topic PALEOHISTOLOGY
VERTEBRATES
TECHNIQUES
THIN SECTIONS
ASPHALT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Paleohistological studies of vertebrates are based on the analysis and interpretation of bone microstructure of petrographic thin sections. These sections are mainly performed following standard techniques; however, the methodology can vary according to the particular characteristics of the sample (e.g. skeletal element, type of preservation) or the purpose of the work. As part of a paleohistological study on Equus occidentalis recovered from a Late Pleistocene asphalt seep ("tar pit"), Rancho La Brea (California, United States), in this contribution we describe the technique used to make thin sections of fossils impregnated with asphalt. The hydrocarbons act as a release agent, which causes the resins commonly used for glued or embedding not to adhere to the fossil remains. For chip preparation (=block) the sample were embedded in a large volume of low-viscosity epoxy resin, which generated a higher shrinkage and prevented the separation of the chip components (resin / fossil). The chip must be mounted on the glass slide using UV acrylic resin, which, unlike epoxy resins, does not require heat application, optimizes adhesion, and reduces the curing time, offering the possibility of cutting and polishing quickly after mounting. During polishing, it was necessary to reduce the cleaning time with ultrasound to avoid peeling and loss of portions of the sample. Based on the results obtained, we consider that this variant of the traditional methodology is optimal to work with fossils preserved in this particular context, as it allows making complete thin sections without altering the original osteohistological features.
Fil: Garrone, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; Argentina
Fil: Ortiz, Herácleo O.. Universidad Nacional del Sur. Departamento de Geología; Argentina
Fil: Prado, José L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; Argentina
2nd Palaeontological Virtual Congress
España
Palaeontological Virtual Congress
description Paleohistological studies of vertebrates are based on the analysis and interpretation of bone microstructure of petrographic thin sections. These sections are mainly performed following standard techniques; however, the methodology can vary according to the particular characteristics of the sample (e.g. skeletal element, type of preservation) or the purpose of the work. As part of a paleohistological study on Equus occidentalis recovered from a Late Pleistocene asphalt seep ("tar pit"), Rancho La Brea (California, United States), in this contribution we describe the technique used to make thin sections of fossils impregnated with asphalt. The hydrocarbons act as a release agent, which causes the resins commonly used for glued or embedding not to adhere to the fossil remains. For chip preparation (=block) the sample were embedded in a large volume of low-viscosity epoxy resin, which generated a higher shrinkage and prevented the separation of the chip components (resin / fossil). The chip must be mounted on the glass slide using UV acrylic resin, which, unlike epoxy resins, does not require heat application, optimizes adhesion, and reduces the curing time, offering the possibility of cutting and polishing quickly after mounting. During polishing, it was necessary to reduce the cleaning time with ultrasound to avoid peeling and loss of portions of the sample. Based on the results obtained, we consider that this variant of the traditional methodology is optimal to work with fossils preserved in this particular context, as it allows making complete thin sections without altering the original osteohistological features.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/175774
Thin sections techniques in fossil remains of mammals impregnated with asphalt; 2nd Palaeontological Virtual Congress; España; 2020; 95-95
978-84-09-20283-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/175774
identifier_str_mv Thin sections techniques in fossil remains of mammals impregnated with asphalt; 2nd Palaeontological Virtual Congress; España; 2020; 95-95
978-84-09-20283-6
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://palaeovc.org/index.php/downloads/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Palaeontological Virtual Congress
publisher.none.fl_str_mv Palaeontological Virtual Congress
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instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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