Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1

Autores
Ariel, Federico Damian; Diet, Anouck; Verdenaud, Marion; Gruber, Véronique; Frugier, Florian; Chan, Raquel Lia; Crespi, Martín
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The adaptation of root architecture to environmental constraints is a major agricultural trait, notably in legumes, the third main crop worldwide. This root developmental plasticity depends on the formation of lateral roots (LRs) emerging from primary roots. In the model legume Medicago truncatula, the HD-Zip I transcription factor HB1 is expressed in primary and lateral root meristems and induced by salt stress. Constitutive expression of HB1 in M. truncatula roots alters their architecture, whereas hb1 TILLING mutants showed increased lateral root emergence. Electrophoretic mobility shift assay, promoter mutagenesis, and chromatin immunoprecipitation-PCR assays revealed that HB1 directly recognizes a CAATAATTG cis-element present in the promoter of a LOB-like (for Lateral Organ Boundaries) gene, LBD1, transcriptionally regulated by auxin. Expression of these genes in response to abscisic acid and auxin and their behavior in hb1 mutants revealed an HB1-mediated repression of LBD1 acting during LR emergence. M. truncatula HB1 regulates an adaptive developmental response to minimize the root surface exposed to adverse environmental stresses.
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Diet, Anouck. Centre National de la Recherche Scientifique; Francia
Fil: Verdenaud, Marion. Institut National de la Recherche Agronomique; Francia
Fil: Gruber, Véronique. Centre National de la Recherche Scientifique; Francia
Fil: Frugier, Florian. Centre National de la Recherche Scientifique; Francia
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Crespi, Martín. Centre National de la Recherche Scientifique; Francia
Materia
Root Development
Transcription Factors
Auxin
Aba
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75953
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/75953
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1Ariel, Federico DamianDiet, AnouckVerdenaud, MarionGruber, VéroniqueFrugier, FlorianChan, Raquel LiaCrespi, MartínRoot DevelopmentTranscription FactorsAuxinAbahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The adaptation of root architecture to environmental constraints is a major agricultural trait, notably in legumes, the third main crop worldwide. This root developmental plasticity depends on the formation of lateral roots (LRs) emerging from primary roots. In the model legume Medicago truncatula, the HD-Zip I transcription factor HB1 is expressed in primary and lateral root meristems and induced by salt stress. Constitutive expression of HB1 in M. truncatula roots alters their architecture, whereas hb1 TILLING mutants showed increased lateral root emergence. Electrophoretic mobility shift assay, promoter mutagenesis, and chromatin immunoprecipitation-PCR assays revealed that HB1 directly recognizes a CAATAATTG cis-element present in the promoter of a LOB-like (for Lateral Organ Boundaries) gene, LBD1, transcriptionally regulated by auxin. Expression of these genes in response to abscisic acid and auxin and their behavior in hb1 mutants revealed an HB1-mediated repression of LBD1 acting during LR emergence. M. truncatula HB1 regulates an adaptive developmental response to minimize the root surface exposed to adverse environmental stresses.Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Diet, Anouck. Centre National de la Recherche Scientifique; FranciaFil: Verdenaud, Marion. Institut National de la Recherche Agronomique; FranciaFil: Gruber, Véronique. Centre National de la Recherche Scientifique; FranciaFil: Frugier, Florian. Centre National de la Recherche Scientifique; FranciaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Crespi, Martín. Centre National de la Recherche Scientifique; FranciaAmerican Society of Plant Biologist2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75953Ariel, Federico Damian; Diet, Anouck; Verdenaud, Marion; Gruber, Véronique; Frugier, Florian; et al.; Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1; American Society of Plant Biologist; Plant Cell; 22; 7; 7-2010; 2171-21831040-46511532-298XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.110.074823info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/22/7/2171info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:54Zoai:ri.conicet.gov.ar:11336/75953instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:55.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
title Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
spellingShingle Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
Ariel, Federico Damian
Root Development
Transcription Factors
Auxin
Aba
title_short Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
title_full Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
title_fullStr Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
title_full_unstemmed Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
title_sort Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1
dc.creator.none.fl_str_mv Ariel, Federico Damian
Diet, Anouck
Verdenaud, Marion
Gruber, Véronique
Frugier, Florian
Chan, Raquel Lia
Crespi, Martín
author Ariel, Federico Damian
author_facet Ariel, Federico Damian
Diet, Anouck
Verdenaud, Marion
Gruber, Véronique
Frugier, Florian
Chan, Raquel Lia
Crespi, Martín
author_role author
author2 Diet, Anouck
Verdenaud, Marion
Gruber, Véronique
Frugier, Florian
Chan, Raquel Lia
Crespi, Martín
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Root Development
Transcription Factors
Auxin
Aba
topic Root Development
Transcription Factors
Auxin
Aba
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The adaptation of root architecture to environmental constraints is a major agricultural trait, notably in legumes, the third main crop worldwide. This root developmental plasticity depends on the formation of lateral roots (LRs) emerging from primary roots. In the model legume Medicago truncatula, the HD-Zip I transcription factor HB1 is expressed in primary and lateral root meristems and induced by salt stress. Constitutive expression of HB1 in M. truncatula roots alters their architecture, whereas hb1 TILLING mutants showed increased lateral root emergence. Electrophoretic mobility shift assay, promoter mutagenesis, and chromatin immunoprecipitation-PCR assays revealed that HB1 directly recognizes a CAATAATTG cis-element present in the promoter of a LOB-like (for Lateral Organ Boundaries) gene, LBD1, transcriptionally regulated by auxin. Expression of these genes in response to abscisic acid and auxin and their behavior in hb1 mutants revealed an HB1-mediated repression of LBD1 acting during LR emergence. M. truncatula HB1 regulates an adaptive developmental response to minimize the root surface exposed to adverse environmental stresses.
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Diet, Anouck. Centre National de la Recherche Scientifique; Francia
Fil: Verdenaud, Marion. Institut National de la Recherche Agronomique; Francia
Fil: Gruber, Véronique. Centre National de la Recherche Scientifique; Francia
Fil: Frugier, Florian. Centre National de la Recherche Scientifique; Francia
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Crespi, Martín. Centre National de la Recherche Scientifique; Francia
description The adaptation of root architecture to environmental constraints is a major agricultural trait, notably in legumes, the third main crop worldwide. This root developmental plasticity depends on the formation of lateral roots (LRs) emerging from primary roots. In the model legume Medicago truncatula, the HD-Zip I transcription factor HB1 is expressed in primary and lateral root meristems and induced by salt stress. Constitutive expression of HB1 in M. truncatula roots alters their architecture, whereas hb1 TILLING mutants showed increased lateral root emergence. Electrophoretic mobility shift assay, promoter mutagenesis, and chromatin immunoprecipitation-PCR assays revealed that HB1 directly recognizes a CAATAATTG cis-element present in the promoter of a LOB-like (for Lateral Organ Boundaries) gene, LBD1, transcriptionally regulated by auxin. Expression of these genes in response to abscisic acid and auxin and their behavior in hb1 mutants revealed an HB1-mediated repression of LBD1 acting during LR emergence. M. truncatula HB1 regulates an adaptive developmental response to minimize the root surface exposed to adverse environmental stresses.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75953
Ariel, Federico Damian; Diet, Anouck; Verdenaud, Marion; Gruber, Véronique; Frugier, Florian; et al.; Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1; American Society of Plant Biologist; Plant Cell; 22; 7; 7-2010; 2171-2183
1040-4651
1532-298X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75953
identifier_str_mv Ariel, Federico Damian; Diet, Anouck; Verdenaud, Marion; Gruber, Véronique; Frugier, Florian; et al.; Environmental regulation of lateral root emergence in Medicago truncatula requires the HD-zip i transcription factor HB1; American Society of Plant Biologist; Plant Cell; 22; 7; 7-2010; 2171-2183
1040-4651
1532-298X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.110.074823
info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/22/7/2171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269312894631936
score 13.13397

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