Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia
- Autores
- Simes, Adriana Beatriz; Maturano, Ramona del Carmen; Tassile, Valentín; Caballero, Adriana Catalina; del Monaco, Silvana Maria
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named ÑNM10, ÑIF8 and ÑMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain ÑMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale.
Fil: Simes, Adriana Beatriz. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina
Fil: Maturano, Ramona del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Tassile, Valentín. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina
Fil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina
Fil: del Monaco, Silvana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina - Materia
-
SACCHAROMYCES
WINE
HEXOSES
TRANSPORT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/62913
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Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North PatagoniaSimes, Adriana BeatrizMaturano, Ramona del CarmenTassile, ValentínCaballero, Adriana Catalinadel Monaco, Silvana MariaSACCHAROMYCESWINEHEXOSESTRANSPORThttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named ÑNM10, ÑIF8 and ÑMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain ÑMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale.Fil: Simes, Adriana Beatriz. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; ArgentinaFil: Maturano, Ramona del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaFil: Tassile, Valentín. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; ArgentinaFil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; ArgentinaFil: del Monaco, Silvana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaScientific Research Publishing2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62913Simes, Adriana Beatriz; Maturano, Ramona del Carmen; Tassile, Valentín; Caballero, Adriana Catalina; del Monaco, Silvana Maria; Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia; Scientific Research Publishing; Advances in Microbiology; 6; 9; 8-2016; 733-744; 698612165-3402CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/aim.2016.69072info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/15-2270788_69861.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:59Zoai:ri.conicet.gov.ar:11336/62913instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:59.882CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
title |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
spellingShingle |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia Simes, Adriana Beatriz SACCHAROMYCES WINE HEXOSES TRANSPORT |
title_short |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
title_full |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
title_fullStr |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
title_full_unstemmed |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
title_sort |
Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia |
dc.creator.none.fl_str_mv |
Simes, Adriana Beatriz Maturano, Ramona del Carmen Tassile, Valentín Caballero, Adriana Catalina del Monaco, Silvana Maria |
author |
Simes, Adriana Beatriz |
author_facet |
Simes, Adriana Beatriz Maturano, Ramona del Carmen Tassile, Valentín Caballero, Adriana Catalina del Monaco, Silvana Maria |
author_role |
author |
author2 |
Maturano, Ramona del Carmen Tassile, Valentín Caballero, Adriana Catalina del Monaco, Silvana Maria |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
SACCHAROMYCES WINE HEXOSES TRANSPORT |
topic |
SACCHAROMYCES WINE HEXOSES TRANSPORT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named ÑNM10, ÑIF8 and ÑMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain ÑMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale. Fil: Simes, Adriana Beatriz. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina Fil: Maturano, Ramona del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina Fil: Tassile, Valentín. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina Fil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias y Tecnologia de los Alimentos; Argentina Fil: del Monaco, Silvana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina |
description |
At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named ÑNM10, ÑIF8 and ÑMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain ÑMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/62913 Simes, Adriana Beatriz; Maturano, Ramona del Carmen; Tassile, Valentín; Caballero, Adriana Catalina; del Monaco, Silvana Maria; Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia; Scientific Research Publishing; Advances in Microbiology; 6; 9; 8-2016; 733-744; 69861 2165-3402 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/62913 |
identifier_str_mv |
Simes, Adriana Beatriz; Maturano, Ramona del Carmen; Tassile, Valentín; Caballero, Adriana Catalina; del Monaco, Silvana Maria; Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia; Scientific Research Publishing; Advances in Microbiology; 6; 9; 8-2016; 733-744; 69861 2165-3402 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4236/aim.2016.69072 info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/15-2270788_69861.htm |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Scientific Research Publishing |
publisher.none.fl_str_mv |
Scientific Research Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269985058062336 |
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13.13397 |