Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid
- Autores
- Pérez Torrado, Roberto; González, Sara Susana; Combina, Mariana; Barrio, Eladio; Querol, Amparo
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Saccharomyces cerevisiae plays a main role in the winemaking process, although other species, like Saccharomyces uvarum or Saccharomyces paradoxus, have been associated with must fermentations. It has been reported in recent years, that yeast hybrids of different Saccharomyces species might be responsible for wine productions. Although S. cerevisiae× Saccharomyces kudriavzevii hybrids have been well studied, very little attention has been paid to S. cerevisiae× S. uvarum hybrids. In this work we characterized the genomic composition of S6U, a widely used commercial S. cerevisiae× S. uvarum yeast hybrid isolated in wine fermentations containing one copy of the genome of each parental species, which suggests a relatively recent hybridization event. We also studied its performance under diverse enological conditions. The results show enhanced performance under low temperature enological conditions, increased glycerol production, lower acetic acid production and increased production of interesting aroma compounds. We also examined the transcriptomic response of the S6U hybrid strain compared with the reference species under enological conditions. The results show that although the hybrid strain transcriptome is more similar to S. uvarum than to S. cerevisiae, it presents specifically regulated genes involved in stress response, lipids and amino acid metabolism. The enological performance and aroma profile of this S. cerevisiae× S. uvarum hybrid makes it a good candidate for participating in winemaking, especially at low temperatures. cerevisiae x S. uvarum yeast maintain the genomes from both parents equally. uvarum than S. cerevisiae (genes involved in stress response, lipids and aminoacid metabolism). cerevisiae x S. uvarum hybrid strain S6U seems better adapted to low and intermediate temperature fermentative conditions.
Fil: Pérez Torrado, Roberto. Instituto de Agroquímica y Tecnología de los Alimentos; España
Fil: González, Sara Susana. Instituto de Agroquímica y Tecnología de los Alimentos; España
Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Agroquímica y Tecnología de los Alimentos; España. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Barrio, Eladio. Instituto de Agroquímica y Tecnología de los Alimentos; España. Universidad de Valencia; España
Fil: Querol, Amparo. Instituto de Agroquímica y Tecnología de los Alimentos; España - Materia
-
Fermentation
Hybrids
Saccharomyces
Transcriptomics
Wine - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/59296
Ver los metadatos del registro completo
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Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybridPérez Torrado, RobertoGonzález, Sara SusanaCombina, MarianaBarrio, EladioQuerol, AmparoFermentationHybridsSaccharomycesTranscriptomicsWinehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Saccharomyces cerevisiae plays a main role in the winemaking process, although other species, like Saccharomyces uvarum or Saccharomyces paradoxus, have been associated with must fermentations. It has been reported in recent years, that yeast hybrids of different Saccharomyces species might be responsible for wine productions. Although S. cerevisiae× Saccharomyces kudriavzevii hybrids have been well studied, very little attention has been paid to S. cerevisiae× S. uvarum hybrids. In this work we characterized the genomic composition of S6U, a widely used commercial S. cerevisiae× S. uvarum yeast hybrid isolated in wine fermentations containing one copy of the genome of each parental species, which suggests a relatively recent hybridization event. We also studied its performance under diverse enological conditions. The results show enhanced performance under low temperature enological conditions, increased glycerol production, lower acetic acid production and increased production of interesting aroma compounds. We also examined the transcriptomic response of the S6U hybrid strain compared with the reference species under enological conditions. The results show that although the hybrid strain transcriptome is more similar to S. uvarum than to S. cerevisiae, it presents specifically regulated genes involved in stress response, lipids and amino acid metabolism. The enological performance and aroma profile of this S. cerevisiae× S. uvarum hybrid makes it a good candidate for participating in winemaking, especially at low temperatures. cerevisiae x S. uvarum yeast maintain the genomes from both parents equally. uvarum than S. cerevisiae (genes involved in stress response, lipids and aminoacid metabolism). cerevisiae x S. uvarum hybrid strain S6U seems better adapted to low and intermediate temperature fermentative conditions.Fil: Pérez Torrado, Roberto. Instituto de Agroquímica y Tecnología de los Alimentos; EspañaFil: González, Sara Susana. Instituto de Agroquímica y Tecnología de los Alimentos; EspañaFil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Agroquímica y Tecnología de los Alimentos; España. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Barrio, Eladio. Instituto de Agroquímica y Tecnología de los Alimentos; España. Universidad de Valencia; EspañaFil: Querol, Amparo. Instituto de Agroquímica y Tecnología de los Alimentos; EspañaElsevier Science2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59296Pérez Torrado, Roberto; González, Sara Susana; Combina, Mariana; Barrio, Eladio; Querol, Amparo; Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid; Elsevier Science; International Journal of Food Microbiology; 204; 7-2015; 101-1100168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2015.03.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160515001518info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:29Zoai:ri.conicet.gov.ar:11336/59296instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:30.391CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
title |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
spellingShingle |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid Pérez Torrado, Roberto Fermentation Hybrids Saccharomyces Transcriptomics Wine |
title_short |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
title_full |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
title_fullStr |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
title_full_unstemmed |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
title_sort |
Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid |
dc.creator.none.fl_str_mv |
Pérez Torrado, Roberto González, Sara Susana Combina, Mariana Barrio, Eladio Querol, Amparo |
author |
Pérez Torrado, Roberto |
author_facet |
Pérez Torrado, Roberto González, Sara Susana Combina, Mariana Barrio, Eladio Querol, Amparo |
author_role |
author |
author2 |
González, Sara Susana Combina, Mariana Barrio, Eladio Querol, Amparo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Fermentation Hybrids Saccharomyces Transcriptomics Wine |
topic |
Fermentation Hybrids Saccharomyces Transcriptomics Wine |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Saccharomyces cerevisiae plays a main role in the winemaking process, although other species, like Saccharomyces uvarum or Saccharomyces paradoxus, have been associated with must fermentations. It has been reported in recent years, that yeast hybrids of different Saccharomyces species might be responsible for wine productions. Although S. cerevisiae× Saccharomyces kudriavzevii hybrids have been well studied, very little attention has been paid to S. cerevisiae× S. uvarum hybrids. In this work we characterized the genomic composition of S6U, a widely used commercial S. cerevisiae× S. uvarum yeast hybrid isolated in wine fermentations containing one copy of the genome of each parental species, which suggests a relatively recent hybridization event. We also studied its performance under diverse enological conditions. The results show enhanced performance under low temperature enological conditions, increased glycerol production, lower acetic acid production and increased production of interesting aroma compounds. We also examined the transcriptomic response of the S6U hybrid strain compared with the reference species under enological conditions. The results show that although the hybrid strain transcriptome is more similar to S. uvarum than to S. cerevisiae, it presents specifically regulated genes involved in stress response, lipids and amino acid metabolism. The enological performance and aroma profile of this S. cerevisiae× S. uvarum hybrid makes it a good candidate for participating in winemaking, especially at low temperatures. cerevisiae x S. uvarum yeast maintain the genomes from both parents equally. uvarum than S. cerevisiae (genes involved in stress response, lipids and aminoacid metabolism). cerevisiae x S. uvarum hybrid strain S6U seems better adapted to low and intermediate temperature fermentative conditions. Fil: Pérez Torrado, Roberto. Instituto de Agroquímica y Tecnología de los Alimentos; España Fil: González, Sara Susana. Instituto de Agroquímica y Tecnología de los Alimentos; España Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Agroquímica y Tecnología de los Alimentos; España. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Barrio, Eladio. Instituto de Agroquímica y Tecnología de los Alimentos; España. Universidad de Valencia; España Fil: Querol, Amparo. Instituto de Agroquímica y Tecnología de los Alimentos; España |
description |
Saccharomyces cerevisiae plays a main role in the winemaking process, although other species, like Saccharomyces uvarum or Saccharomyces paradoxus, have been associated with must fermentations. It has been reported in recent years, that yeast hybrids of different Saccharomyces species might be responsible for wine productions. Although S. cerevisiae× Saccharomyces kudriavzevii hybrids have been well studied, very little attention has been paid to S. cerevisiae× S. uvarum hybrids. In this work we characterized the genomic composition of S6U, a widely used commercial S. cerevisiae× S. uvarum yeast hybrid isolated in wine fermentations containing one copy of the genome of each parental species, which suggests a relatively recent hybridization event. We also studied its performance under diverse enological conditions. The results show enhanced performance under low temperature enological conditions, increased glycerol production, lower acetic acid production and increased production of interesting aroma compounds. We also examined the transcriptomic response of the S6U hybrid strain compared with the reference species under enological conditions. The results show that although the hybrid strain transcriptome is more similar to S. uvarum than to S. cerevisiae, it presents specifically regulated genes involved in stress response, lipids and amino acid metabolism. The enological performance and aroma profile of this S. cerevisiae× S. uvarum hybrid makes it a good candidate for participating in winemaking, especially at low temperatures. cerevisiae x S. uvarum yeast maintain the genomes from both parents equally. uvarum than S. cerevisiae (genes involved in stress response, lipids and aminoacid metabolism). cerevisiae x S. uvarum hybrid strain S6U seems better adapted to low and intermediate temperature fermentative conditions. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/59296 Pérez Torrado, Roberto; González, Sara Susana; Combina, Mariana; Barrio, Eladio; Querol, Amparo; Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid; Elsevier Science; International Journal of Food Microbiology; 204; 7-2015; 101-110 0168-1605 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/59296 |
identifier_str_mv |
Pérez Torrado, Roberto; González, Sara Susana; Combina, Mariana; Barrio, Eladio; Querol, Amparo; Molecular and enological characterization of a natural Saccharomyces uvarum and Saccharomyces cerevisiae hybrid; Elsevier Science; International Journal of Food Microbiology; 204; 7-2015; 101-110 0168-1605 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2015.03.012 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160515001518 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268927286050816 |
score |
13.13397 |