PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects

Autores
Monesterolo, Noelia Edith; Nigra, Ayelén D.; Campetelli, Alexis Nazareno; Santander, Verónica Silvina; Rivelli Antonelli, Juan Franco; Arce, Carlos Angel; Casale, Cesar Horacio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our previous studies demonstrated formation of a complex between acetylated tubulin and brain plasma membrane Ca2 +-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and on PMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase in membrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin in normotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) and phosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associated with tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlated with erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatment with DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicate that PMCA activity is involved in deformability of both normotensive and hypertensive erythrocytes. This rheological property of erythrocytes is affected by acetylated tubulin and its lipid environment because both regulate PMCA activity.
Fil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Nigra, Ayelén D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Campetelli, Alexis Nazareno. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Santander, Verónica Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivelli Antonelli, Juan Franco. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Arce, Carlos Angel. Universidad Nacional de Córdoba. Facultad de Cs.químicas. Departamento de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Casale, Cesar Horacio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Materia
Diacylglycerol
Hypertension
Phosphatidic Acid
Pmca
Regulation
Tubulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50830
Acceder
id CONICETDig_27cc415364c0df4708d0d36223777143
oai_identifier_str oai:ri.conicet.gov.ar:11336/50830
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjectsMonesterolo, Noelia EdithNigra, Ayelén D.Campetelli, Alexis NazarenoSantander, Verónica SilvinaRivelli Antonelli, Juan FrancoArce, Carlos AngelCasale, Cesar HoracioDiacylglycerolHypertensionPhosphatidic AcidPmcaRegulationTubulinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Our previous studies demonstrated formation of a complex between acetylated tubulin and brain plasma membrane Ca2 +-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and on PMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase in membrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin in normotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) and phosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associated with tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlated with erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatment with DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicate that PMCA activity is involved in deformability of both normotensive and hypertensive erythrocytes. This rheological property of erythrocytes is affected by acetylated tubulin and its lipid environment because both regulate PMCA activity.Fil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Nigra, Ayelén D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Campetelli, Alexis Nazareno. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Santander, Verónica Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rivelli Antonelli, Juan Franco. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Arce, Carlos Angel. Universidad Nacional de Córdoba. Facultad de Cs.químicas. Departamento de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Casale, Cesar Horacio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier Science2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50830Monesterolo, Noelia Edith; Nigra, Ayelén D.; Campetelli, Alexis Nazareno; Santander, Verónica Silvina; Rivelli Antonelli, Juan Franco; et al.; PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1848; 11; 11-2015; 2813-28200005-2736CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S000527361500262Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2015.08.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:07Zoai:ri.conicet.gov.ar:11336/50830instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:34:08.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
title PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
spellingShingle PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
Monesterolo, Noelia Edith
Diacylglycerol
Hypertension
Phosphatidic Acid
Pmca
Regulation
Tubulin
title_short PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
title_full PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
title_fullStr PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
title_full_unstemmed PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
title_sort PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects
dc.creator.none.fl_str_mv Monesterolo, Noelia Edith
Nigra, Ayelén D.
Campetelli, Alexis Nazareno
Santander, Verónica Silvina
Rivelli Antonelli, Juan Franco
Arce, Carlos Angel
Casale, Cesar Horacio
author Monesterolo, Noelia Edith
author_facet Monesterolo, Noelia Edith
Nigra, Ayelén D.
Campetelli, Alexis Nazareno
Santander, Verónica Silvina
Rivelli Antonelli, Juan Franco
Arce, Carlos Angel
Casale, Cesar Horacio
author_role author
author2 Nigra, Ayelén D.
Campetelli, Alexis Nazareno
Santander, Verónica Silvina
Rivelli Antonelli, Juan Franco
Arce, Carlos Angel
Casale, Cesar Horacio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Diacylglycerol
Hypertension
Phosphatidic Acid
Pmca
Regulation
Tubulin
topic Diacylglycerol
Hypertension
Phosphatidic Acid
Pmca
Regulation
Tubulin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Our previous studies demonstrated formation of a complex between acetylated tubulin and brain plasma membrane Ca2 +-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and on PMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase in membrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin in normotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) and phosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associated with tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlated with erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatment with DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicate that PMCA activity is involved in deformability of both normotensive and hypertensive erythrocytes. This rheological property of erythrocytes is affected by acetylated tubulin and its lipid environment because both regulate PMCA activity.
Fil: Monesterolo, Noelia Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Nigra, Ayelén D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Campetelli, Alexis Nazareno. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Santander, Verónica Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivelli Antonelli, Juan Franco. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Arce, Carlos Angel. Universidad Nacional de Córdoba. Facultad de Cs.químicas. Departamento de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Casale, Cesar Horacio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
description Our previous studies demonstrated formation of a complex between acetylated tubulin and brain plasma membrane Ca2 +-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and on PMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase in membrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin in normotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) and phosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associated with tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlated with erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatment with DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicate that PMCA activity is involved in deformability of both normotensive and hypertensive erythrocytes. This rheological property of erythrocytes is affected by acetylated tubulin and its lipid environment because both regulate PMCA activity.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50830
Monesterolo, Noelia Edith; Nigra, Ayelén D.; Campetelli, Alexis Nazareno; Santander, Verónica Silvina; Rivelli Antonelli, Juan Franco; et al.; PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1848; 11; 11-2015; 2813-2820
0005-2736
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50830
identifier_str_mv Monesterolo, Noelia Edith; Nigra, Ayelén D.; Campetelli, Alexis Nazareno; Santander, Verónica Silvina; Rivelli Antonelli, Juan Franco; et al.; PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1848; 11; 11-2015; 2813-2820
0005-2736
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S000527361500262X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2015.08.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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