A cohort-based study of host gene expression: tumor suppressor and innate immune/inflammatory pathways associated with the HIV reservoir size

• Autores: Dwivedi, Ashok K.; Gornalusse, Germán G.; Siegel, David A.; Barbehenn, Alton; Thanh, Cassandra; Hoh, Rebecca; Hobbs, Kristen S.; Pan, Tony; Gibson, Erica A.; Martin, Jeffrey; Hecht, Frederick; Pilcher, Christopher; Milush, Jeffrey; Busch, Michael P.; Stone, Mars; Huang, Meei Li; Reppetti, Julieta; Vo, Phuong M.; Levy, Claire N.; Roychoudhury, Pavitra; Jerome, Keith R.; Hladik, Florian; Henrich, Timothy J.; Deeks, Steven G.; Lee, Sulggi A.
• Año de publicación: 2023
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: HIV RESERVOIR; IMMUNOLOGY
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The major barrier to an HIV cure is the HIV reservoir: latently-infected cells that persist despite effective antiretroviral therapy (ART). There have been few cohort-based studies evaluating host genomic or transcriptomic predictors of the HIV reservoir. We performed host RNA sequencing and HIV reservoir quantification (total DNA [tDNA], unspliced RNA [usRNA], intact DNA) from peripheral CD4+ T cells from 191 ART-suppressed people with HIV (PWH). After adjusting for nadir CD4+ count, timing of ART initiation, and genetic ancestry, we identified two host genes for which higher expression was significantly associated with smaller total DNA viral reservoir size, P3H3 and NBL1, both known tumor suppressor genes. We then identified 17 host genes for which lower expression was associated with higher residual transcription (HIV usRNA). These included novel associations with membrane channel (KCNJ2, GJB2), inflammasome (IL1A, CSF3, TNFAIP5, TNFAIP6, TNFAIP9, CXCL3, CXCL10), and innate immunity (TLR7) genes (FDR-adjusted q<0.05). Gene set enrichment analyses further identified significant associations of HIV usRNA with TLR4/microbial translocation (q = 0.006), IL-1/NRLP3 inflammasome (q = 0.008), and IL-10 (q = 0.037) signaling. Protein validation assays using ELISA and multiplex cytokine assays supported these observed inverse host gene correlations, with P3H3, IL-10, and TNF-α protein associations achieving statistical significance (p<0.05). Plasma IL-10 was also significantly inversely associated with HIV DNA (p = 0.016). HIV intact DNA was not associated with differential host gene expression, although this may have been due to a large number of undetectable values in our study. To our knowledge, this is the largest host transcriptomic study of the HIV reservoir. Our findings suggest that host gene expression may vary in response to the transcriptionally active reservoir and that changes in cellular proliferation genes may influence the size of the HIV reservoir. These findings add important data to the limited host genetic HIV reservoir studies to date.
Fil: Dwivedi, Ashok K.. University of California; Estados Unidos
Fil: Gornalusse, Germán G.. University of Washington; Estados Unidos
Fil: Siegel, David A.. University of California; Estados Unidos
Fil: Barbehenn, Alton. University of California; Estados Unidos
Fil: Thanh, Cassandra. University of California; Estados Unidos
Fil: Hoh, Rebecca. University of California; Estados Unidos
Fil: Hobbs, Kristen S.. University of California; Estados Unidos
Fil: Pan, Tony. University of California; Estados Unidos
Fil: Gibson, Erica A.. University of California; Estados Unidos
Fil: Martin, Jeffrey. University of California; Estados Unidos
Fil: Hecht, Frederick. University of California; Estados Unidos
Fil: Pilcher, Christopher. University of California; Estados Unidos
Fil: Milush, Jeffrey. University of California; Estados Unidos
Fil: Busch, Michael P.. Vitalant Blood Bank; Estados Unidos
Fil: Stone, Mars. Vitalant Blood Bank; Estados Unidos
Fil: Huang, Meei Li. University of Washington; Estados Unidos
Fil: Reppetti, Julieta. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Vo, Phuong M.. University of Washington; Estados Unidos
Fil: Levy, Claire N.. University of Washington; Estados Unidos
Fil: Roychoudhury, Pavitra. University of Washington; Estados Unidos
Fil: Jerome, Keith R.. University of Washington; Estados Unidos
Fil: Hladik, Florian. University of Washington. School of Medicine; Estados Unidos
Fil: Henrich, Timothy J.. University of Washington. School of Medicine; Estados Unidos
Fil: Deeks, Steven G.. University of California; Estados Unidos
Fil: Lee, Sulggi A.. University of California; Estados Unidos