Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2

Autores
Bissinger, Rosi; Lang, Elisabeth; Ghashghaeinia, Mehrdad; Singh, Yogesh; Zelenak, Christine; Fehrenbacher, Birgit; Honisch, Sabina; Chen, Hong; Fakhri, Hajar; Umbach, Anja T.; Liu, Guilai; Rexhepaj, Rexhep; Liu, Guoxing; Schaller, Martin; Mack, Andreas F.; Lupescu, Adrian; Birnbaumer, Lutz; Lang, Florian; Qadri, Syed M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca2+ activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2−/−) and corresponding wild-type mice (Gαi2+/+). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2−/− and Gαi2+/+ mice but the mean corpuscular volume was significantly larger in Gαi2−/− mice. Spontaneous PS exposure of circulating Gαi2−/− erythrocytes was significantly lower than that of circulating Gαi2+/+ erythrocytes. PS exposure was significantly lower in Gαi2−/− than in Gαi2+/+ erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca2+ activity and cell shrinkage. Moreover, Gαi2−/− erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2+/+ erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death.
Fil: Bissinger, Rosi. Eberhard Karls Universität Tübingen; Alemania
Fil: Lang, Elisabeth. Universitat Dusseldorf; Alemania
Fil: Ghashghaeinia, Mehrdad. Eberhard Karls Universität Tübingen; Alemania
Fil: Singh, Yogesh. Eberhard Karls Universität Tübingen; Alemania
Fil: Zelenak, Christine. Charité Medical University; Alemania
Fil: Fehrenbacher, Birgit. Eberhard Karls Universität Tübingen; Alemania
Fil: Honisch, Sabina. Eberhard Karls Universität Tübingen; Alemania
Fil: Chen, Hong. Eberhard Karls Universität Tübingen; Alemania
Fil: Fakhri, Hajar. Eberhard Karls Universität Tübingen; Alemania
Fil: Umbach, Anja T.. Eberhard Karls Universität Tübingen; Alemania
Fil: Liu, Guilai. Eberhard Karls Universität Tübingen; Alemania
Fil: Rexhepaj, Rexhep. Universitat Bonn; Alemania
Fil: Liu, Guoxing. Eberhard Karls Universität Tübingen; Alemania
Fil: Schaller, Martin. Eberhard Karls Universität Tübingen; Alemania
Fil: Mack, Andreas F.. Eberhard Karls Universität Tübingen; Alemania
Fil: Lupescu, Adrian. Eberhard Karls Universität Tübingen; Alemania
Fil: Birnbaumer, Lutz. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lang, Florian. Eberhard Karls Universität Tübingen; Alemania
Fil: Qadri, Syed M.. Eberhard Karls Universität Tübingen; Alemania. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Materia
Gi2alpha
G Protein
Erythocyte
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40386
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40386
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2Bissinger, RosiLang, ElisabethGhashghaeinia, MehrdadSingh, YogeshZelenak, ChristineFehrenbacher, BirgitHonisch, SabinaChen, HongFakhri, HajarUmbach, Anja T.Liu, GuilaiRexhepaj, RexhepLiu, GuoxingSchaller, MartinMack, Andreas F.Lupescu, AdrianBirnbaumer, LutzLang, FlorianQadri, Syed M.Gi2alphaG ProteinErythocyteApoptosishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca2+ activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2−/−) and corresponding wild-type mice (Gαi2+/+). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2−/− and Gαi2+/+ mice but the mean corpuscular volume was significantly larger in Gαi2−/− mice. Spontaneous PS exposure of circulating Gαi2−/− erythrocytes was significantly lower than that of circulating Gαi2+/+ erythrocytes. PS exposure was significantly lower in Gαi2−/− than in Gαi2+/+ erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca2+ activity and cell shrinkage. Moreover, Gαi2−/− erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2+/+ erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death.Fil: Bissinger, Rosi. Eberhard Karls Universität Tübingen; AlemaniaFil: Lang, Elisabeth. Universitat Dusseldorf; AlemaniaFil: Ghashghaeinia, Mehrdad. Eberhard Karls Universität Tübingen; AlemaniaFil: Singh, Yogesh. Eberhard Karls Universität Tübingen; AlemaniaFil: Zelenak, Christine. Charité Medical University; AlemaniaFil: Fehrenbacher, Birgit. Eberhard Karls Universität Tübingen; AlemaniaFil: Honisch, Sabina. Eberhard Karls Universität Tübingen; AlemaniaFil: Chen, Hong. Eberhard Karls Universität Tübingen; AlemaniaFil: Fakhri, Hajar. Eberhard Karls Universität Tübingen; AlemaniaFil: Umbach, Anja T.. Eberhard Karls Universität Tübingen; AlemaniaFil: Liu, Guilai. Eberhard Karls Universität Tübingen; AlemaniaFil: Rexhepaj, Rexhep. Universitat Bonn; AlemaniaFil: Liu, Guoxing. Eberhard Karls Universität Tübingen; AlemaniaFil: Schaller, Martin. Eberhard Karls Universität Tübingen; AlemaniaFil: Mack, Andreas F.. Eberhard Karls Universität Tübingen; AlemaniaFil: Lupescu, Adrian. Eberhard Karls Universität Tübingen; AlemaniaFil: Birnbaumer, Lutz. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lang, Florian. Eberhard Karls Universität Tübingen; AlemaniaFil: Qadri, Syed M.. Eberhard Karls Universität Tübingen; Alemania. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaNature Publishing Group2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40386Bissinger, Rosi; Lang, Elisabeth; Ghashghaeinia, Mehrdad; Singh, Yogesh; Zelenak, Christine; et al.; Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2; Nature Publishing Group; Scientific Reports; 6; 8-2016; 1-10; 309252045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep30925info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep30925info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:44Zoai:ri.conicet.gov.ar:11336/40386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:44.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
spellingShingle Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
Bissinger, Rosi
Gi2alpha
G Protein
Erythocyte
Apoptosis
title_short Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_full Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_fullStr Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_full_unstemmed Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_sort Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
dc.creator.none.fl_str_mv Bissinger, Rosi
Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
author Bissinger, Rosi
author_facet Bissinger, Rosi
Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
author_role author
author2 Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gi2alpha
G Protein
Erythocyte
Apoptosis
topic Gi2alpha
G Protein
Erythocyte
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca2+ activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2−/−) and corresponding wild-type mice (Gαi2+/+). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2−/− and Gαi2+/+ mice but the mean corpuscular volume was significantly larger in Gαi2−/− mice. Spontaneous PS exposure of circulating Gαi2−/− erythrocytes was significantly lower than that of circulating Gαi2+/+ erythrocytes. PS exposure was significantly lower in Gαi2−/− than in Gαi2+/+ erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca2+ activity and cell shrinkage. Moreover, Gαi2−/− erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2+/+ erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death.
Fil: Bissinger, Rosi. Eberhard Karls Universität Tübingen; Alemania
Fil: Lang, Elisabeth. Universitat Dusseldorf; Alemania
Fil: Ghashghaeinia, Mehrdad. Eberhard Karls Universität Tübingen; Alemania
Fil: Singh, Yogesh. Eberhard Karls Universität Tübingen; Alemania
Fil: Zelenak, Christine. Charité Medical University; Alemania
Fil: Fehrenbacher, Birgit. Eberhard Karls Universität Tübingen; Alemania
Fil: Honisch, Sabina. Eberhard Karls Universität Tübingen; Alemania
Fil: Chen, Hong. Eberhard Karls Universität Tübingen; Alemania
Fil: Fakhri, Hajar. Eberhard Karls Universität Tübingen; Alemania
Fil: Umbach, Anja T.. Eberhard Karls Universität Tübingen; Alemania
Fil: Liu, Guilai. Eberhard Karls Universität Tübingen; Alemania
Fil: Rexhepaj, Rexhep. Universitat Bonn; Alemania
Fil: Liu, Guoxing. Eberhard Karls Universität Tübingen; Alemania
Fil: Schaller, Martin. Eberhard Karls Universität Tübingen; Alemania
Fil: Mack, Andreas F.. Eberhard Karls Universität Tübingen; Alemania
Fil: Lupescu, Adrian. Eberhard Karls Universität Tübingen; Alemania
Fil: Birnbaumer, Lutz. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lang, Florian. Eberhard Karls Universität Tübingen; Alemania
Fil: Qadri, Syed M.. Eberhard Karls Universität Tübingen; Alemania. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
description Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca2+ activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2−/−) and corresponding wild-type mice (Gαi2+/+). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2−/− and Gαi2+/+ mice but the mean corpuscular volume was significantly larger in Gαi2−/− mice. Spontaneous PS exposure of circulating Gαi2−/− erythrocytes was significantly lower than that of circulating Gαi2+/+ erythrocytes. PS exposure was significantly lower in Gαi2−/− than in Gαi2+/+ erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca2+ activity and cell shrinkage. Moreover, Gαi2−/− erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2+/+ erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40386
Bissinger, Rosi; Lang, Elisabeth; Ghashghaeinia, Mehrdad; Singh, Yogesh; Zelenak, Christine; et al.; Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2; Nature Publishing Group; Scientific Reports; 6; 8-2016; 1-10; 30925
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40386
identifier_str_mv Bissinger, Rosi; Lang, Elisabeth; Ghashghaeinia, Mehrdad; Singh, Yogesh; Zelenak, Christine; et al.; Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2; Nature Publishing Group; Scientific Reports; 6; 8-2016; 1-10; 30925
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep30925
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep30925
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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