Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi

Autores
Ibarrola Vannucci, Ana K.; De Pablos, Luis M.; Retana Moreira, Lissette; Cornet Gomez, Alberto; Cruz Bustos, Teresa; Schijman, Alejandro Gabriel; Ramírez, José L.; Vílchez, Susana; Osuna, Antonio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. cruzi PHBs revealed a pleiotropic function in different stages of the parasite, participating actively in the transformation of the non-infective replicative epimastigote form into metacyclic trypomastigotes and also in the multiplication of intracellular amastigotes. Methodology/principal findings To obtain and confirm our results, we applied several tools and techniques such as electron microscopy, immuno-electron microscopy, bioinformatics analysis and molecular biology. We transfected T. cruzi clones with the PHB genes, in order to overexpress the proteins and performed a CRISPR/Cas9 disruption to obtain partially silenced PHB1 parasites or completely silenced PHB2 parasites. The function of these proteins was also studied in the biology of the parasite, specifically in the transformation rate from non-infective forms to the metacyclic infective forms, and in their capacity of intracellular multiplication. Conclusion/significance This research expands our understanding of the functions of PHBs in the life cycle of the parasite. It also highlights the protective role of prohibitins against ROS and reveals that the absence of PHB2 has a lethal effect on the parasite, a fact that could support the consideration of this protein as a possible target for therapeutic action.
Fil: Ibarrola Vannucci, Ana K.. Universidad de Granada; España
Fil: De Pablos, Luis M.. Universidad de Granada; España
Fil: Retana Moreira, Lissette. Universidad de Granada; España
Fil: Cornet Gomez, Alberto. Universidad de Granada; España
Fil: Cruz Bustos, Teresa. Universidad de Granada; España
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ramírez, José L.. Universidad Central de Venezuela; Venezuela
Fil: Vílchez, Susana. Universidad de Granada; España
Fil: Osuna, Antonio. Universidad de Granada; España
Materia
TRYPANOSOMA CRUZI
CHAGAS
PROHIBITINA 1
PROHIBITINA 2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158975
Acceder
id CONICETDig_2655709b7fd42b4780e478dfbe08c618
oai_identifier_str oai:ri.conicet.gov.ar:11336/158975
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruziIbarrola Vannucci, Ana K.De Pablos, Luis M.Retana Moreira, LissetteCornet Gomez, AlbertoCruz Bustos, TeresaSchijman, Alejandro GabrielRamírez, José L.Vílchez, SusanaOsuna, AntonioTRYPANOSOMA CRUZICHAGASPROHIBITINA 1PROHIBITINA 2https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. cruzi PHBs revealed a pleiotropic function in different stages of the parasite, participating actively in the transformation of the non-infective replicative epimastigote form into metacyclic trypomastigotes and also in the multiplication of intracellular amastigotes. Methodology/principal findings To obtain and confirm our results, we applied several tools and techniques such as electron microscopy, immuno-electron microscopy, bioinformatics analysis and molecular biology. We transfected T. cruzi clones with the PHB genes, in order to overexpress the proteins and performed a CRISPR/Cas9 disruption to obtain partially silenced PHB1 parasites or completely silenced PHB2 parasites. The function of these proteins was also studied in the biology of the parasite, specifically in the transformation rate from non-infective forms to the metacyclic infective forms, and in their capacity of intracellular multiplication. Conclusion/significance This research expands our understanding of the functions of PHBs in the life cycle of the parasite. It also highlights the protective role of prohibitins against ROS and reveals that the absence of PHB2 has a lethal effect on the parasite, a fact that could support the consideration of this protein as a possible target for therapeutic action.Fil: Ibarrola Vannucci, Ana K.. Universidad de Granada; EspañaFil: De Pablos, Luis M.. Universidad de Granada; EspañaFil: Retana Moreira, Lissette. Universidad de Granada; EspañaFil: Cornet Gomez, Alberto. Universidad de Granada; EspañaFil: Cruz Bustos, Teresa. Universidad de Granada; EspañaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ramírez, José L.. Universidad Central de Venezuela; VenezuelaFil: Vílchez, Susana. Universidad de Granada; EspañaFil: Osuna, Antonio. Universidad de Granada; EspañaPublic Library of Science2021-04-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158975Ibarrola Vannucci, Ana K.; De Pablos, Luis M.; Retana Moreira, Lissette; Cornet Gomez, Alberto; Cruz Bustos, Teresa; et al.; Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi; Public Library of Science; Neglected Tropical Diseases; 15; 4; 8-4-2021; 1-291935-27271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0009322info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009322info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:35:24Zoai:ri.conicet.gov.ar:11336/158975instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:35:24.893CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
title Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
spellingShingle Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
Ibarrola Vannucci, Ana K.
TRYPANOSOMA CRUZI
CHAGAS
PROHIBITINA 1
PROHIBITINA 2
title_short Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
title_full Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
title_fullStr Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
title_full_unstemmed Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
title_sort Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi
dc.creator.none.fl_str_mv Ibarrola Vannucci, Ana K.
De Pablos, Luis M.
Retana Moreira, Lissette
Cornet Gomez, Alberto
Cruz Bustos, Teresa
Schijman, Alejandro Gabriel
Ramírez, José L.
Vílchez, Susana
Osuna, Antonio
author Ibarrola Vannucci, Ana K.
author_facet Ibarrola Vannucci, Ana K.
De Pablos, Luis M.
Retana Moreira, Lissette
Cornet Gomez, Alberto
Cruz Bustos, Teresa
Schijman, Alejandro Gabriel
Ramírez, José L.
Vílchez, Susana
Osuna, Antonio
author_role author
author2 De Pablos, Luis M.
Retana Moreira, Lissette
Cornet Gomez, Alberto
Cruz Bustos, Teresa
Schijman, Alejandro Gabriel
Ramírez, José L.
Vílchez, Susana
Osuna, Antonio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
CHAGAS
PROHIBITINA 1
PROHIBITINA 2
topic TRYPANOSOMA CRUZI
CHAGAS
PROHIBITINA 1
PROHIBITINA 2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. cruzi PHBs revealed a pleiotropic function in different stages of the parasite, participating actively in the transformation of the non-infective replicative epimastigote form into metacyclic trypomastigotes and also in the multiplication of intracellular amastigotes. Methodology/principal findings To obtain and confirm our results, we applied several tools and techniques such as electron microscopy, immuno-electron microscopy, bioinformatics analysis and molecular biology. We transfected T. cruzi clones with the PHB genes, in order to overexpress the proteins and performed a CRISPR/Cas9 disruption to obtain partially silenced PHB1 parasites or completely silenced PHB2 parasites. The function of these proteins was also studied in the biology of the parasite, specifically in the transformation rate from non-infective forms to the metacyclic infective forms, and in their capacity of intracellular multiplication. Conclusion/significance This research expands our understanding of the functions of PHBs in the life cycle of the parasite. It also highlights the protective role of prohibitins against ROS and reveals that the absence of PHB2 has a lethal effect on the parasite, a fact that could support the consideration of this protein as a possible target for therapeutic action.
Fil: Ibarrola Vannucci, Ana K.. Universidad de Granada; España
Fil: De Pablos, Luis M.. Universidad de Granada; España
Fil: Retana Moreira, Lissette. Universidad de Granada; España
Fil: Cornet Gomez, Alberto. Universidad de Granada; España
Fil: Cruz Bustos, Teresa. Universidad de Granada; España
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ramírez, José L.. Universidad Central de Venezuela; Venezuela
Fil: Vílchez, Susana. Universidad de Granada; España
Fil: Osuna, Antonio. Universidad de Granada; España
description Background Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. cruzi PHBs revealed a pleiotropic function in different stages of the parasite, participating actively in the transformation of the non-infective replicative epimastigote form into metacyclic trypomastigotes and also in the multiplication of intracellular amastigotes. Methodology/principal findings To obtain and confirm our results, we applied several tools and techniques such as electron microscopy, immuno-electron microscopy, bioinformatics analysis and molecular biology. We transfected T. cruzi clones with the PHB genes, in order to overexpress the proteins and performed a CRISPR/Cas9 disruption to obtain partially silenced PHB1 parasites or completely silenced PHB2 parasites. The function of these proteins was also studied in the biology of the parasite, specifically in the transformation rate from non-infective forms to the metacyclic infective forms, and in their capacity of intracellular multiplication. Conclusion/significance This research expands our understanding of the functions of PHBs in the life cycle of the parasite. It also highlights the protective role of prohibitins against ROS and reveals that the absence of PHB2 has a lethal effect on the parasite, a fact that could support the consideration of this protein as a possible target for therapeutic action.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158975
Ibarrola Vannucci, Ana K.; De Pablos, Luis M.; Retana Moreira, Lissette; Cornet Gomez, Alberto; Cruz Bustos, Teresa; et al.; Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi; Public Library of Science; Neglected Tropical Diseases; 15; 4; 8-4-2021; 1-29
1935-2727
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158975
identifier_str_mv Ibarrola Vannucci, Ana K.; De Pablos, Luis M.; Retana Moreira, Lissette; Cornet Gomez, Alberto; Cruz Bustos, Teresa; et al.; Characterization and functional analysis of the proteins prohibitin 1 and 2 in trypanosoma cruzi; Public Library of Science; Neglected Tropical Diseases; 15; 4; 8-4-2021; 1-29
1935-2727
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0009322
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009322
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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