Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses

Autores
Fidelis Marinho, Claudia; Cônsoli, Fernando L.; Penteado-Dias, Angélica Maria; Zucchi, Roberto A.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study clarifies the identity of two morphs that appear similar but not identical to the braconid wasp Doryctobracon areolatus (Szépligeti), by analyses of fore wing morphometry and the ITS2 and D2 region of 28S ribosomal DNA. Wing measurements of D. areolatus and the two morphs, one with a yellow stigma and one with a brown stigma, from several Brazilian localities, were taken and subjected to geometric morphometry. Evaluation of 20 anatomical points on the fore wing by means of multivariate analysis revealed that these morphs consistently differ from D. areolatus. Intraspecific size variation in both molecular markers (ITS2 and 28S-D2 rDNA) was also detected among populations of D. areolatus from the states of Amapá, São Paulo, Goiás, and Tocantins, but no such difference was observed among samples of the two morphs. However, high sequence variability was observed for both markers among D. areolatus and these morphs. Morphometric analysis yielded similar results and produced dendrogram congruent with those based on the molecular markers, also indicating that D. areolatus corresponds to a complex of cryptic species. Therefore, our morphometric and molecular data, in addition to revealing that D. areolatus includes cryptic species, showed that the unidentified morphs actually represent two distinct, previously undescribed species of Doryctobracon. These new taxa are formally described herein, as D. whartoni sp. nov. and D. adaimei sp. nov.
Fil: Fidelis Marinho, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
Fil: Cônsoli, Fernando L.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
Fil: Penteado-Dias, Angélica Maria. Universidade Federal do São Carlos; Brasil
Fil: Zucchi, Roberto A.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
Materia
28S-D2 RDNA
CANONICAL VARIATE ANALYSIS
GEOMETRIC MORPHOMETRY
ITS2
MOLECULAR TAXONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72123
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72123
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analysesFidelis Marinho, ClaudiaCônsoli, Fernando L.Penteado-Dias, Angélica MariaZucchi, Roberto A.28S-D2 RDNACANONICAL VARIATE ANALYSISGEOMETRIC MORPHOMETRYITS2MOLECULAR TAXONOMYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study clarifies the identity of two morphs that appear similar but not identical to the braconid wasp Doryctobracon areolatus (Szépligeti), by analyses of fore wing morphometry and the ITS2 and D2 region of 28S ribosomal DNA. Wing measurements of D. areolatus and the two morphs, one with a yellow stigma and one with a brown stigma, from several Brazilian localities, were taken and subjected to geometric morphometry. Evaluation of 20 anatomical points on the fore wing by means of multivariate analysis revealed that these morphs consistently differ from D. areolatus. Intraspecific size variation in both molecular markers (ITS2 and 28S-D2 rDNA) was also detected among populations of D. areolatus from the states of Amapá, São Paulo, Goiás, and Tocantins, but no such difference was observed among samples of the two morphs. However, high sequence variability was observed for both markers among D. areolatus and these morphs. Morphometric analysis yielded similar results and produced dendrogram congruent with those based on the molecular markers, also indicating that D. areolatus corresponds to a complex of cryptic species. Therefore, our morphometric and molecular data, in addition to revealing that D. areolatus includes cryptic species, showed that the unidentified morphs actually represent two distinct, previously undescribed species of Doryctobracon. These new taxa are formally described herein, as D. whartoni sp. nov. and D. adaimei sp. nov.Fil: Fidelis Marinho, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; BrasilFil: Cônsoli, Fernando L.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; BrasilFil: Penteado-Dias, Angélica Maria. Universidade Federal do São Carlos; BrasilFil: Zucchi, Roberto A.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; BrasilMagnolia Press2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72123Fidelis Marinho, Claudia; Cônsoli, Fernando L.; Penteado-Dias, Angélica Maria; Zucchi, Roberto A.; Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses; Magnolia Press; Zootaxa; 4353; 3; 11-2017; 467-4841175-5326CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/Zootaxa/article/view/zootaxa.4353.3.4info:eu-repo/semantics/altIdentifier/doi/10.11646/zootaxa.4353.3.4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:34Zoai:ri.conicet.gov.ar:11336/72123instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:34.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
title Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
spellingShingle Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
Fidelis Marinho, Claudia
28S-D2 RDNA
CANONICAL VARIATE ANALYSIS
GEOMETRIC MORPHOMETRY
ITS2
MOLECULAR TAXONOMY
title_short Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
title_full Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
title_fullStr Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
title_full_unstemmed Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
title_sort Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses
dc.creator.none.fl_str_mv Fidelis Marinho, Claudia
Cônsoli, Fernando L.
Penteado-Dias, Angélica Maria
Zucchi, Roberto A.
author Fidelis Marinho, Claudia
author_facet Fidelis Marinho, Claudia
Cônsoli, Fernando L.
Penteado-Dias, Angélica Maria
Zucchi, Roberto A.
author_role author
author2 Cônsoli, Fernando L.
Penteado-Dias, Angélica Maria
Zucchi, Roberto A.
author2_role author
author
author
dc.subject.none.fl_str_mv 28S-D2 RDNA
CANONICAL VARIATE ANALYSIS
GEOMETRIC MORPHOMETRY
ITS2
MOLECULAR TAXONOMY
topic 28S-D2 RDNA
CANONICAL VARIATE ANALYSIS
GEOMETRIC MORPHOMETRY
ITS2
MOLECULAR TAXONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study clarifies the identity of two morphs that appear similar but not identical to the braconid wasp Doryctobracon areolatus (Szépligeti), by analyses of fore wing morphometry and the ITS2 and D2 region of 28S ribosomal DNA. Wing measurements of D. areolatus and the two morphs, one with a yellow stigma and one with a brown stigma, from several Brazilian localities, were taken and subjected to geometric morphometry. Evaluation of 20 anatomical points on the fore wing by means of multivariate analysis revealed that these morphs consistently differ from D. areolatus. Intraspecific size variation in both molecular markers (ITS2 and 28S-D2 rDNA) was also detected among populations of D. areolatus from the states of Amapá, São Paulo, Goiás, and Tocantins, but no such difference was observed among samples of the two morphs. However, high sequence variability was observed for both markers among D. areolatus and these morphs. Morphometric analysis yielded similar results and produced dendrogram congruent with those based on the molecular markers, also indicating that D. areolatus corresponds to a complex of cryptic species. Therefore, our morphometric and molecular data, in addition to revealing that D. areolatus includes cryptic species, showed that the unidentified morphs actually represent two distinct, previously undescribed species of Doryctobracon. These new taxa are formally described herein, as D. whartoni sp. nov. and D. adaimei sp. nov.
Fil: Fidelis Marinho, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
Fil: Cônsoli, Fernando L.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
Fil: Penteado-Dias, Angélica Maria. Universidade Federal do São Carlos; Brasil
Fil: Zucchi, Roberto A.. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil
description This study clarifies the identity of two morphs that appear similar but not identical to the braconid wasp Doryctobracon areolatus (Szépligeti), by analyses of fore wing morphometry and the ITS2 and D2 region of 28S ribosomal DNA. Wing measurements of D. areolatus and the two morphs, one with a yellow stigma and one with a brown stigma, from several Brazilian localities, were taken and subjected to geometric morphometry. Evaluation of 20 anatomical points on the fore wing by means of multivariate analysis revealed that these morphs consistently differ from D. areolatus. Intraspecific size variation in both molecular markers (ITS2 and 28S-D2 rDNA) was also detected among populations of D. areolatus from the states of Amapá, São Paulo, Goiás, and Tocantins, but no such difference was observed among samples of the two morphs. However, high sequence variability was observed for both markers among D. areolatus and these morphs. Morphometric analysis yielded similar results and produced dendrogram congruent with those based on the molecular markers, also indicating that D. areolatus corresponds to a complex of cryptic species. Therefore, our morphometric and molecular data, in addition to revealing that D. areolatus includes cryptic species, showed that the unidentified morphs actually represent two distinct, previously undescribed species of Doryctobracon. These new taxa are formally described herein, as D. whartoni sp. nov. and D. adaimei sp. nov.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72123
Fidelis Marinho, Claudia; Cônsoli, Fernando L.; Penteado-Dias, Angélica Maria; Zucchi, Roberto A.; Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses; Magnolia Press; Zootaxa; 4353; 3; 11-2017; 467-484
1175-5326
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72123
identifier_str_mv Fidelis Marinho, Claudia; Cônsoli, Fernando L.; Penteado-Dias, Angélica Maria; Zucchi, Roberto A.; Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses; Magnolia Press; Zootaxa; 4353; 3; 11-2017; 467-484
1175-5326
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/Zootaxa/article/view/zootaxa.4353.3.4
info:eu-repo/semantics/altIdentifier/doi/10.11646/zootaxa.4353.3.4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Magnolia Press
publisher.none.fl_str_mv Magnolia Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082979149905920
score 12.891075

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