Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules

Autores
Zell, Martina B.; Fahnenstich, Holger; Maier, Alexandra; Saigo, Mariana; Voznesenskaya, Elena v.; Edwards, Gerald E.; Andreo, Carlos Santiago; Schleifenbaum, Frank; Zell, Christiane; Drincovich, Maria Fabiana; Maurino, Verónica G.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C3 plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C3 plant. When grown in short days (SD), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both SD and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in SD MEm plants are a consequence of impairment in the supply of carbon skeletons during a long dark period. This carbon starvation phenotype observed at the end of the night demonstrates a physiological role of the C4 acids, which may be a constitutive function in plants.
Fil: Zell, Martina B.. Universitat Zu Köln; Alemania
Fil: Fahnenstich, Holger. Universitat Zu Köln; Alemania
Fil: Maier, Alexandra. Universitat Zu Köln; Alemania
Fil: Saigo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Voznesenskaya, Elena v.. Russian Academy of Sciences; Rusia
Fil: Edwards, Gerald E.. Washington State University; Estados Unidos
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Schleifenbaum, Frank. Universität Tübingen; Alemania
Fil: Zell, Christiane. Universität Tübingen; Alemania
Fil: Drincovich, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Maurino, Verónica G.. Universitat Zu Köln; Alemania
Materia
Organic Acids
Carbon Metabolism
Dark Metabolism
Short Days
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15453
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15453
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon moleculesZell, Martina B.Fahnenstich, HolgerMaier, AlexandraSaigo, MarianaVoznesenskaya, Elena v.Edwards, Gerald E.Andreo, Carlos SantiagoSchleifenbaum, FrankZell, ChristianeDrincovich, Maria FabianaMaurino, Verónica G.Organic AcidsCarbon MetabolismDark MetabolismShort Dayshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C3 plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C3 plant. When grown in short days (SD), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both SD and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in SD MEm plants are a consequence of impairment in the supply of carbon skeletons during a long dark period. This carbon starvation phenotype observed at the end of the night demonstrates a physiological role of the C4 acids, which may be a constitutive function in plants.Fil: Zell, Martina B.. Universitat Zu Köln; AlemaniaFil: Fahnenstich, Holger. Universitat Zu Köln; AlemaniaFil: Maier, Alexandra. Universitat Zu Köln; AlemaniaFil: Saigo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; ArgentinaFil: Voznesenskaya, Elena v.. Russian Academy of Sciences; RusiaFil: Edwards, Gerald E.. Washington State University; Estados UnidosFil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; ArgentinaFil: Schleifenbaum, Frank. Universität Tübingen; AlemaniaFil: Zell, Christiane. Universität Tübingen; AlemaniaFil: Drincovich, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; ArgentinaFil: Maurino, Verónica G.. Universitat Zu Köln; AlemaniaAmerican Society Of Plant Biologist2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15453Zell, Martina B.; Fahnenstich, Holger; Maier, Alexandra; Saigo, Mariana; Voznesenskaya, Elena v.; et al.; Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules; American Society Of Plant Biologist; Plant Physiology; 152; 3; 3-2010; 1251-12621532-2548enginfo:eu-repo/semantics/altIdentifier/doi/10.​1104/​pp.​109.​151795info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/152/3/1251info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:19Zoai:ri.conicet.gov.ar:11336/15453instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:19.615CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
title Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
spellingShingle Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
Zell, Martina B.
Organic Acids
Carbon Metabolism
Dark Metabolism
Short Days
title_short Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
title_full Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
title_fullStr Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
title_full_unstemmed Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
title_sort Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules
dc.creator.none.fl_str_mv Zell, Martina B.
Fahnenstich, Holger
Maier, Alexandra
Saigo, Mariana
Voznesenskaya, Elena v.
Edwards, Gerald E.
Andreo, Carlos Santiago
Schleifenbaum, Frank
Zell, Christiane
Drincovich, Maria Fabiana
Maurino, Verónica G.
author Zell, Martina B.
author_facet Zell, Martina B.
Fahnenstich, Holger
Maier, Alexandra
Saigo, Mariana
Voznesenskaya, Elena v.
Edwards, Gerald E.
Andreo, Carlos Santiago
Schleifenbaum, Frank
Zell, Christiane
Drincovich, Maria Fabiana
Maurino, Verónica G.
author_role author
author2 Fahnenstich, Holger
Maier, Alexandra
Saigo, Mariana
Voznesenskaya, Elena v.
Edwards, Gerald E.
Andreo, Carlos Santiago
Schleifenbaum, Frank
Zell, Christiane
Drincovich, Maria Fabiana
Maurino, Verónica G.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Organic Acids
Carbon Metabolism
Dark Metabolism
Short Days
topic Organic Acids
Carbon Metabolism
Dark Metabolism
Short Days
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C3 plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C3 plant. When grown in short days (SD), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both SD and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in SD MEm plants are a consequence of impairment in the supply of carbon skeletons during a long dark period. This carbon starvation phenotype observed at the end of the night demonstrates a physiological role of the C4 acids, which may be a constitutive function in plants.
Fil: Zell, Martina B.. Universitat Zu Köln; Alemania
Fil: Fahnenstich, Holger. Universitat Zu Köln; Alemania
Fil: Maier, Alexandra. Universitat Zu Köln; Alemania
Fil: Saigo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Voznesenskaya, Elena v.. Russian Academy of Sciences; Rusia
Fil: Edwards, Gerald E.. Washington State University; Estados Unidos
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Schleifenbaum, Frank. Universität Tübingen; Alemania
Fil: Zell, Christiane. Universität Tübingen; Alemania
Fil: Drincovich, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Maurino, Verónica G.. Universitat Zu Köln; Alemania
description While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C3 plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C3 plant. When grown in short days (SD), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both SD and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in SD MEm plants are a consequence of impairment in the supply of carbon skeletons during a long dark period. This carbon starvation phenotype observed at the end of the night demonstrates a physiological role of the C4 acids, which may be a constitutive function in plants.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15453
Zell, Martina B.; Fahnenstich, Holger; Maier, Alexandra; Saigo, Mariana; Voznesenskaya, Elena v.; et al.; Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules; American Society Of Plant Biologist; Plant Physiology; 152; 3; 3-2010; 1251-1262
1532-2548
url http://hdl.handle.net/11336/15453
identifier_str_mv Zell, Martina B.; Fahnenstich, Holger; Maier, Alexandra; Saigo, Mariana; Voznesenskaya, Elena v.; et al.; Analysis of Arabidopsis with highly reduced levels of malate and fumarate sheds light on the role of these organic acids as storage carbon molecules; American Society Of Plant Biologist; Plant Physiology; 152; 3; 3-2010; 1251-1262
1532-2548
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.​1104/​pp.​109.​151795
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/152/3/1251
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society Of Plant Biologist
publisher.none.fl_str_mv American Society Of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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