ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss

Autores
Montaña, Sabrina Daiana; Almuzara, Marisa; Pennini, M; Sucari, A.; Centron, Daniela; Vay, C.; Ramirez, Maria Soledad
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work is to study the dispersion of two ISs, ISCR2 and IS26, which are known to contribute in the acquisition of resistance determinants and genome plasticity, among a collection of hundred and seventy-four Acinetobacter spp. clinical isolates. PCR amplification reactions using total DNA were performed to search ISCR2, IS26, and different antibiotic resistance genes (tet(B), aphA6, sul2, floR, dfr9) previously described in the genetic context of ISCR2.Among A. baumannii, positive amplification for ISCR2 was obtained in 66% of the included isolates and most of them (93%) were positive for IS26 amplification. The platform tet(B)::ISCR2 was found in 57% of the ISCR2 positive isolates and only 14 gave negative amplification for tet(B). In these isolates positive amplifications of genes that were previously described associated to ISCR2 -such as, aphA6, sul2, floR, and dfr9- were observed. When searching these ISs in the non-baumannii Acinetobacter studied-collection, two isolates were positive for ISCR2 and two isolates for IS26. No positive results were obtained for tet(B), but the presence of sul2, floR and dfr9 was found. Our results exposed a great dispersion of ISCR2 as well as IS26 among Acinetobacter spp. clinical isolates reinforcing the idea that the ISs play a crucial role in the plasticity and evolution towards drug resistance in this genus.
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Almuzara, Marisa. Laboratorio de Bacteriología; Argentina
Fil: Pennini, M. Unidad Microbiología; Argentina
Fil: Sucari, A.. Unidad Microbiología; Argentina
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Vay, C.. Laboratorio de Bacteriología; Argentina
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
Acinetobacter spp
ISCR2
IS26
Insertion sequences
Minimum inhibitory concentration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47504
Acceder
id CONICETDig_25254e7ff9ba041e07b8ffee74b84f91
oai_identifier_str oai:ri.conicet.gov.ar:11336/47504
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical StrainssMontaña, Sabrina DaianaAlmuzara, MarisaPennini, MSucari, A.Centron, DanielaVay, C.Ramirez, Maria SoledadAcinetobacter sppISCR2IS26Insertion sequencesMinimum inhibitory concentrationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work is to study the dispersion of two ISs, ISCR2 and IS26, which are known to contribute in the acquisition of resistance determinants and genome plasticity, among a collection of hundred and seventy-four Acinetobacter spp. clinical isolates. PCR amplification reactions using total DNA were performed to search ISCR2, IS26, and different antibiotic resistance genes (tet(B), aphA6, sul2, floR, dfr9) previously described in the genetic context of ISCR2.Among A. baumannii, positive amplification for ISCR2 was obtained in 66% of the included isolates and most of them (93%) were positive for IS26 amplification. The platform tet(B)::ISCR2 was found in 57% of the ISCR2 positive isolates and only 14 gave negative amplification for tet(B). In these isolates positive amplifications of genes that were previously described associated to ISCR2 -such as, aphA6, sul2, floR, and dfr9- were observed. When searching these ISs in the non-baumannii Acinetobacter studied-collection, two isolates were positive for ISCR2 and two isolates for IS26. No positive results were obtained for tet(B), but the presence of sul2, floR and dfr9 was found. Our results exposed a great dispersion of ISCR2 as well as IS26 among Acinetobacter spp. clinical isolates reinforcing the idea that the ISs play a crucial role in the plasticity and evolution towards drug resistance in this genus.Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Almuzara, Marisa. Laboratorio de Bacteriología; ArgentinaFil: Pennini, M. Unidad Microbiología; ArgentinaFil: Sucari, A.. Unidad Microbiología; ArgentinaFil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Vay, C.. Laboratorio de Bacteriología; ArgentinaFil: Ramirez, Maria Soledad. California State University; Estados UnidosMedCrave2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47504Montaña, Sabrina Daiana; Almuzara, Marisa; Pennini, M; Sucari, A.; Centron, Daniela; et al.; ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss; MedCrave; Journal of Bacteriology and Mycology: Open Acces; 4; 1; 1-2017; 1-42469-2786CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15406/jbmoa.2017.04.00082info:eu-repo/semantics/altIdentifier/url/http://medcraveonline.com/JBMOA/JBMOA-04-00082.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:28Zoai:ri.conicet.gov.ar:11336/47504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:28.581CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
title ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
spellingShingle ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
Montaña, Sabrina Daiana
Acinetobacter spp
ISCR2
IS26
Insertion sequences
Minimum inhibitory concentration
title_short ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
title_full ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
title_fullStr ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
title_full_unstemmed ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
title_sort ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss
dc.creator.none.fl_str_mv Montaña, Sabrina Daiana
Almuzara, Marisa
Pennini, M
Sucari, A.
Centron, Daniela
Vay, C.
Ramirez, Maria Soledad
author Montaña, Sabrina Daiana
author_facet Montaña, Sabrina Daiana
Almuzara, Marisa
Pennini, M
Sucari, A.
Centron, Daniela
Vay, C.
Ramirez, Maria Soledad
author_role author
author2 Almuzara, Marisa
Pennini, M
Sucari, A.
Centron, Daniela
Vay, C.
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acinetobacter spp
ISCR2
IS26
Insertion sequences
Minimum inhibitory concentration
topic Acinetobacter spp
ISCR2
IS26
Insertion sequences
Minimum inhibitory concentration
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work is to study the dispersion of two ISs, ISCR2 and IS26, which are known to contribute in the acquisition of resistance determinants and genome plasticity, among a collection of hundred and seventy-four Acinetobacter spp. clinical isolates. PCR amplification reactions using total DNA were performed to search ISCR2, IS26, and different antibiotic resistance genes (tet(B), aphA6, sul2, floR, dfr9) previously described in the genetic context of ISCR2.Among A. baumannii, positive amplification for ISCR2 was obtained in 66% of the included isolates and most of them (93%) were positive for IS26 amplification. The platform tet(B)::ISCR2 was found in 57% of the ISCR2 positive isolates and only 14 gave negative amplification for tet(B). In these isolates positive amplifications of genes that were previously described associated to ISCR2 -such as, aphA6, sul2, floR, and dfr9- were observed. When searching these ISs in the non-baumannii Acinetobacter studied-collection, two isolates were positive for ISCR2 and two isolates for IS26. No positive results were obtained for tet(B), but the presence of sul2, floR and dfr9 was found. Our results exposed a great dispersion of ISCR2 as well as IS26 among Acinetobacter spp. clinical isolates reinforcing the idea that the ISs play a crucial role in the plasticity and evolution towards drug resistance in this genus.
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Almuzara, Marisa. Laboratorio de Bacteriología; Argentina
Fil: Pennini, M. Unidad Microbiología; Argentina
Fil: Sucari, A.. Unidad Microbiología; Argentina
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Vay, C.. Laboratorio de Bacteriología; Argentina
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description The aim of this work is to study the dispersion of two ISs, ISCR2 and IS26, which are known to contribute in the acquisition of resistance determinants and genome plasticity, among a collection of hundred and seventy-four Acinetobacter spp. clinical isolates. PCR amplification reactions using total DNA were performed to search ISCR2, IS26, and different antibiotic resistance genes (tet(B), aphA6, sul2, floR, dfr9) previously described in the genetic context of ISCR2.Among A. baumannii, positive amplification for ISCR2 was obtained in 66% of the included isolates and most of them (93%) were positive for IS26 amplification. The platform tet(B)::ISCR2 was found in 57% of the ISCR2 positive isolates and only 14 gave negative amplification for tet(B). In these isolates positive amplifications of genes that were previously described associated to ISCR2 -such as, aphA6, sul2, floR, and dfr9- were observed. When searching these ISs in the non-baumannii Acinetobacter studied-collection, two isolates were positive for ISCR2 and two isolates for IS26. No positive results were obtained for tet(B), but the presence of sul2, floR and dfr9 was found. Our results exposed a great dispersion of ISCR2 as well as IS26 among Acinetobacter spp. clinical isolates reinforcing the idea that the ISs play a crucial role in the plasticity and evolution towards drug resistance in this genus.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47504
Montaña, Sabrina Daiana; Almuzara, Marisa; Pennini, M; Sucari, A.; Centron, Daniela; et al.; ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss; MedCrave; Journal of Bacteriology and Mycology: Open Acces; 4; 1; 1-2017; 1-4
2469-2786
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47504
identifier_str_mv Montaña, Sabrina Daiana; Almuzara, Marisa; Pennini, M; Sucari, A.; Centron, Daniela; et al.; ISCR2 and IS26: Two Insertion Sequences Highly Dispersed among Acinetobacter spp. Clinical Strainss; MedCrave; Journal of Bacteriology and Mycology: Open Acces; 4; 1; 1-2017; 1-4
2469-2786
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15406/jbmoa.2017.04.00082
info:eu-repo/semantics/altIdentifier/url/http://medcraveonline.com/JBMOA/JBMOA-04-00082.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MedCrave
publisher.none.fl_str_mv MedCrave
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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