Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM

Autores
Rivas Aiello, Maria Belen; Kirse, Thomas M; Lavorato, Gabriel Carlos; Maus, Bastian; Maisuls, Iván; Kuberasivakumaran, Shivadharshini; Ostendorp, Stefan; Hepp, Alexander; Holtkamp, Michael; Winkler, Elin Lilian; Karst, Uwe; Wilde, Gerhard; Faber, Cornelius; Vericat, Carolina; Strassert, Cristian A.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two different hybrid nanosystems are prepared by loading highly crystalline, monodisperse magnetite nanocubes (MNCs) with phosphorescent Pt(II) complexes (PtCxs). One involves the encapsulation of the hydrophobic PtCx1 within an amphiphilic comb polymer (MNC@poly(maleic anhydride-alt-1-octadecene) [PMAO]–PtCx1), whereas the other involves the direct binding of the hydrophilic PtCx2 to the surface of the MNC mediated by a ligand-exchange procedure (MNC@OH–PtCx2). Both systems are evaluated as potential candidates for multimodal imaging in magnetic resonance imaging (MRI) and photoluminescence lifetime imaging micro(spectro)scopy (PLIM). PLIM measurements on agarose phantoms demonstrate significantly longer excited-state lifetimes compared to the short-lived autofluorescence of biological background. Additionally, both nanosystems perform as effective MRI contrast agents (CAs): the r2* values are 3–4 times higher than for the commercial CA ferucarbotran. MNC@PMAO–PtCx1 particles also cause significant increases in r2. While the ligand exchange procedure efficiently anchors PtCxs to the MNC surface, the polymeric encapsulation ensures higher colloidal stability, contributing to differences in PLIM and MRI outcomes. In these results, the successful integration of two complementary noninvasive imaging modalities within a single nanosystem is confirmed, serving as the impetus for further investigation of such systems as advanced multimodal–multiscale imaging agents with dual orthogonal readouts.
Fil: Rivas Aiello, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Kirse, Thomas M. Westfälische Wilhelms Universität; Alemania
Fil: Lavorato, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Maus, Bastian. Universitätsklinikum Münster; Alemania
Fil: Maisuls, Iván. Universitätsklinikum Münster; Alemania
Fil: Kuberasivakumaran, Shivadharshini. Westfälische Wilhelms Universität; Alemania
Fil: Ostendorp, Stefan. Universitätsklinikum Münster; Alemania
Fil: Hepp, Alexander. Westfälische Wilhelms Universität; Alemania
Fil: Holtkamp, Michael. Westfälische Wilhelms Universität; Alemania
Fil: Winkler, Elin Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche | Comisión Nacional de Energía Atómica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche; Argentina
Fil: Karst, Uwe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilde, Gerhard. Westfälische Wilhelms Universität; Alemania
Fil: Faber, Cornelius. Westfälische Wilhelms Universität; Alemania
Fil: Vericat, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Strassert, Cristian A.. Westfälische Wilhelms Universität; Alemania
Materia
MAGNETIC NANOHYBRIDS
MAGNETIC RESONANCE IMAGING (MRI)
NANOPARTICLES
PHOSPHORESCENT PT(II) COMPLEXES
PHOTOLUMINESCENCE LIFETIME IMAGING MICRO(SPECTRO)SCOPY (PLIM)
TIME-RESOLVED MICROSCOPY
TIME-RESOLVED SPECTROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262374
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262374
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIMRivas Aiello, Maria BelenKirse, Thomas MLavorato, Gabriel CarlosMaus, BastianMaisuls, IvánKuberasivakumaran, ShivadharshiniOstendorp, StefanHepp, AlexanderHoltkamp, MichaelWinkler, Elin LilianKarst, UweWilde, GerhardFaber, CorneliusVericat, CarolinaStrassert, Cristian A.MAGNETIC NANOHYBRIDSMAGNETIC RESONANCE IMAGING (MRI)NANOPARTICLESPHOSPHORESCENT PT(II) COMPLEXESPHOTOLUMINESCENCE LIFETIME IMAGING MICRO(SPECTRO)SCOPY (PLIM)TIME-RESOLVED MICROSCOPYTIME-RESOLVED SPECTROSCOPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Two different hybrid nanosystems are prepared by loading highly crystalline, monodisperse magnetite nanocubes (MNCs) with phosphorescent Pt(II) complexes (PtCxs). One involves the encapsulation of the hydrophobic PtCx1 within an amphiphilic comb polymer (MNC@poly(maleic anhydride-alt-1-octadecene) [PMAO]–PtCx1), whereas the other involves the direct binding of the hydrophilic PtCx2 to the surface of the MNC mediated by a ligand-exchange procedure (MNC@OH–PtCx2). Both systems are evaluated as potential candidates for multimodal imaging in magnetic resonance imaging (MRI) and photoluminescence lifetime imaging micro(spectro)scopy (PLIM). PLIM measurements on agarose phantoms demonstrate significantly longer excited-state lifetimes compared to the short-lived autofluorescence of biological background. Additionally, both nanosystems perform as effective MRI contrast agents (CAs): the r2* values are 3–4 times higher than for the commercial CA ferucarbotran. MNC@PMAO–PtCx1 particles also cause significant increases in r2. While the ligand exchange procedure efficiently anchors PtCxs to the MNC surface, the polymeric encapsulation ensures higher colloidal stability, contributing to differences in PLIM and MRI outcomes. In these results, the successful integration of two complementary noninvasive imaging modalities within a single nanosystem is confirmed, serving as the impetus for further investigation of such systems as advanced multimodal–multiscale imaging agents with dual orthogonal readouts.Fil: Rivas Aiello, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Kirse, Thomas M. Westfälische Wilhelms Universität; AlemaniaFil: Lavorato, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Maus, Bastian. Universitätsklinikum Münster; AlemaniaFil: Maisuls, Iván. Universitätsklinikum Münster; AlemaniaFil: Kuberasivakumaran, Shivadharshini. Westfälische Wilhelms Universität; AlemaniaFil: Ostendorp, Stefan. Universitätsklinikum Münster; AlemaniaFil: Hepp, Alexander. Westfälische Wilhelms Universität; AlemaniaFil: Holtkamp, Michael. Westfälische Wilhelms Universität; AlemaniaFil: Winkler, Elin Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche | Comisión Nacional de Energía Atómica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche; ArgentinaFil: Karst, Uwe. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wilde, Gerhard. Westfälische Wilhelms Universität; AlemaniaFil: Faber, Cornelius. Westfälische Wilhelms Universität; AlemaniaFil: Vericat, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Strassert, Cristian A.. Westfälische Wilhelms Universität; AlemaniaJohn Wiley & Sons2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262374Rivas Aiello, Maria Belen; Kirse, Thomas M; Lavorato, Gabriel Carlos; Maus, Bastian; Maisuls, Iván; et al.; Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM; John Wiley & Sons; Small Science; 4; 3; 2-2024; 1-112688-4046CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/smsc.202300145info:eu-repo/semantics/altIdentifier/doi/10.1002/smsc.202300145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:03Zoai:ri.conicet.gov.ar:11336/262374instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:03.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
title Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
spellingShingle Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
Rivas Aiello, Maria Belen
MAGNETIC NANOHYBRIDS
MAGNETIC RESONANCE IMAGING (MRI)
NANOPARTICLES
PHOSPHORESCENT PT(II) COMPLEXES
PHOTOLUMINESCENCE LIFETIME IMAGING MICRO(SPECTRO)SCOPY (PLIM)
TIME-RESOLVED MICROSCOPY
TIME-RESOLVED SPECTROSCOPY
title_short Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
title_full Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
title_fullStr Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
title_full_unstemmed Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
title_sort Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM
dc.creator.none.fl_str_mv Rivas Aiello, Maria Belen
Kirse, Thomas M
Lavorato, Gabriel Carlos
Maus, Bastian
Maisuls, Iván
Kuberasivakumaran, Shivadharshini
Ostendorp, Stefan
Hepp, Alexander
Holtkamp, Michael
Winkler, Elin Lilian
Karst, Uwe
Wilde, Gerhard
Faber, Cornelius
Vericat, Carolina
Strassert, Cristian A.
author Rivas Aiello, Maria Belen
author_facet Rivas Aiello, Maria Belen
Kirse, Thomas M
Lavorato, Gabriel Carlos
Maus, Bastian
Maisuls, Iván
Kuberasivakumaran, Shivadharshini
Ostendorp, Stefan
Hepp, Alexander
Holtkamp, Michael
Winkler, Elin Lilian
Karst, Uwe
Wilde, Gerhard
Faber, Cornelius
Vericat, Carolina
Strassert, Cristian A.
author_role author
author2 Kirse, Thomas M
Lavorato, Gabriel Carlos
Maus, Bastian
Maisuls, Iván
Kuberasivakumaran, Shivadharshini
Ostendorp, Stefan
Hepp, Alexander
Holtkamp, Michael
Winkler, Elin Lilian
Karst, Uwe
Wilde, Gerhard
Faber, Cornelius
Vericat, Carolina
Strassert, Cristian A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MAGNETIC NANOHYBRIDS
MAGNETIC RESONANCE IMAGING (MRI)
NANOPARTICLES
PHOSPHORESCENT PT(II) COMPLEXES
PHOTOLUMINESCENCE LIFETIME IMAGING MICRO(SPECTRO)SCOPY (PLIM)
TIME-RESOLVED MICROSCOPY
TIME-RESOLVED SPECTROSCOPY
topic MAGNETIC NANOHYBRIDS
MAGNETIC RESONANCE IMAGING (MRI)
NANOPARTICLES
PHOSPHORESCENT PT(II) COMPLEXES
PHOTOLUMINESCENCE LIFETIME IMAGING MICRO(SPECTRO)SCOPY (PLIM)
TIME-RESOLVED MICROSCOPY
TIME-RESOLVED SPECTROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two different hybrid nanosystems are prepared by loading highly crystalline, monodisperse magnetite nanocubes (MNCs) with phosphorescent Pt(II) complexes (PtCxs). One involves the encapsulation of the hydrophobic PtCx1 within an amphiphilic comb polymer (MNC@poly(maleic anhydride-alt-1-octadecene) [PMAO]–PtCx1), whereas the other involves the direct binding of the hydrophilic PtCx2 to the surface of the MNC mediated by a ligand-exchange procedure (MNC@OH–PtCx2). Both systems are evaluated as potential candidates for multimodal imaging in magnetic resonance imaging (MRI) and photoluminescence lifetime imaging micro(spectro)scopy (PLIM). PLIM measurements on agarose phantoms demonstrate significantly longer excited-state lifetimes compared to the short-lived autofluorescence of biological background. Additionally, both nanosystems perform as effective MRI contrast agents (CAs): the r2* values are 3–4 times higher than for the commercial CA ferucarbotran. MNC@PMAO–PtCx1 particles also cause significant increases in r2. While the ligand exchange procedure efficiently anchors PtCxs to the MNC surface, the polymeric encapsulation ensures higher colloidal stability, contributing to differences in PLIM and MRI outcomes. In these results, the successful integration of two complementary noninvasive imaging modalities within a single nanosystem is confirmed, serving as the impetus for further investigation of such systems as advanced multimodal–multiscale imaging agents with dual orthogonal readouts.
Fil: Rivas Aiello, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Kirse, Thomas M. Westfälische Wilhelms Universität; Alemania
Fil: Lavorato, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Maus, Bastian. Universitätsklinikum Münster; Alemania
Fil: Maisuls, Iván. Universitätsklinikum Münster; Alemania
Fil: Kuberasivakumaran, Shivadharshini. Westfälische Wilhelms Universität; Alemania
Fil: Ostendorp, Stefan. Universitätsklinikum Münster; Alemania
Fil: Hepp, Alexander. Westfälische Wilhelms Universität; Alemania
Fil: Holtkamp, Michael. Westfälische Wilhelms Universität; Alemania
Fil: Winkler, Elin Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche | Comisión Nacional de Energía Atómica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche; Argentina
Fil: Karst, Uwe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilde, Gerhard. Westfälische Wilhelms Universität; Alemania
Fil: Faber, Cornelius. Westfälische Wilhelms Universität; Alemania
Fil: Vericat, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Strassert, Cristian A.. Westfälische Wilhelms Universität; Alemania
description Two different hybrid nanosystems are prepared by loading highly crystalline, monodisperse magnetite nanocubes (MNCs) with phosphorescent Pt(II) complexes (PtCxs). One involves the encapsulation of the hydrophobic PtCx1 within an amphiphilic comb polymer (MNC@poly(maleic anhydride-alt-1-octadecene) [PMAO]–PtCx1), whereas the other involves the direct binding of the hydrophilic PtCx2 to the surface of the MNC mediated by a ligand-exchange procedure (MNC@OH–PtCx2). Both systems are evaluated as potential candidates for multimodal imaging in magnetic resonance imaging (MRI) and photoluminescence lifetime imaging micro(spectro)scopy (PLIM). PLIM measurements on agarose phantoms demonstrate significantly longer excited-state lifetimes compared to the short-lived autofluorescence of biological background. Additionally, both nanosystems perform as effective MRI contrast agents (CAs): the r2* values are 3–4 times higher than for the commercial CA ferucarbotran. MNC@PMAO–PtCx1 particles also cause significant increases in r2. While the ligand exchange procedure efficiently anchors PtCxs to the MNC surface, the polymeric encapsulation ensures higher colloidal stability, contributing to differences in PLIM and MRI outcomes. In these results, the successful integration of two complementary noninvasive imaging modalities within a single nanosystem is confirmed, serving as the impetus for further investigation of such systems as advanced multimodal–multiscale imaging agents with dual orthogonal readouts.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262374
Rivas Aiello, Maria Belen; Kirse, Thomas M; Lavorato, Gabriel Carlos; Maus, Bastian; Maisuls, Iván; et al.; Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM; John Wiley & Sons; Small Science; 4; 3; 2-2024; 1-11
2688-4046
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262374
identifier_str_mv Rivas Aiello, Maria Belen; Kirse, Thomas M; Lavorato, Gabriel Carlos; Maus, Bastian; Maisuls, Iván; et al.; Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM; John Wiley & Sons; Small Science; 4; 3; 2-2024; 1-11
2688-4046
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/smsc.202300145
info:eu-repo/semantics/altIdentifier/doi/10.1002/smsc.202300145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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