Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses

Autores
Decena, Maria Angeles; Gálvez Rojas, Sergio; Agostini, Federico; Sancho, Ruben; Contreras Moreira, Bruno; Des Marais, David L.; Hernandez, Pilar; Catalán, Pilar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachy-podium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.
Fil: Decena, Maria Angeles. Universidad de Zaragoza; España
Fil: Gálvez Rojas, Sergio. Universidad de Málaga; España
Fil: Agostini, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sancho, Ruben. Universidad de Zaragoza; España
Fil: Contreras Moreira, Bruno. Consejo Superior de Investigaciones Científicas; España
Fil: Des Marais, David L.. Massachusetts Institute of Technology; Reino Unido
Fil: Hernandez, Pilar. Consejo Superior de Investigaciones Científicas; España
Fil: Catalán, Pilar. Universidad de Zaragoza; España
Materia
BDHN GENES
CIS-REGULATORY ELEMENTS
COMPARATIVE GENOMICS
DEHYDRIN STRUCTURE
DEHYDRIN-GENE EXPRESSION
DROUGHT-RELATED TRAITS
DROUGHT-TOLERANT ECOTYPES
DUPLICATED GENES
PHYLOGENETICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168078
Acceder
id CONICETDig_248c2a069308682dda769bd88487cc1c
oai_identifier_str oai:ri.conicet.gov.ar:11336/168078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium GrassesDecena, Maria AngelesGálvez Rojas, SergioAgostini, FedericoSancho, RubenContreras Moreira, BrunoDes Marais, David L.Hernandez, PilarCatalán, PilarBDHN GENESCIS-REGULATORY ELEMENTSCOMPARATIVE GENOMICSDEHYDRIN STRUCTUREDEHYDRIN-GENE EXPRESSIONDROUGHT-RELATED TRAITSDROUGHT-TOLERANT ECOTYPESDUPLICATED GENESPHYLOGENETICShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachy-podium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.Fil: Decena, Maria Angeles. Universidad de Zaragoza; EspañaFil: Gálvez Rojas, Sergio. Universidad de Málaga; EspañaFil: Agostini, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Sancho, Ruben. Universidad de Zaragoza; EspañaFil: Contreras Moreira, Bruno. Consejo Superior de Investigaciones Científicas; EspañaFil: Des Marais, David L.. Massachusetts Institute of Technology; Reino UnidoFil: Hernandez, Pilar. Consejo Superior de Investigaciones Científicas; EspañaFil: Catalán, Pilar. Universidad de Zaragoza; EspañaMDPI2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168078Decena, Maria Angeles; Gálvez Rojas, Sergio; Agostini, Federico; Sancho, Ruben; Contreras Moreira, Bruno; et al.; Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses; MDPI; Plants; 10; 12; 12-2021; 1-292223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/12/2664info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10122664info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:19:35Zoai:ri.conicet.gov.ar:11336/168078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:19:35.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
title Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
spellingShingle Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
Decena, Maria Angeles
BDHN GENES
CIS-REGULATORY ELEMENTS
COMPARATIVE GENOMICS
DEHYDRIN STRUCTURE
DEHYDRIN-GENE EXPRESSION
DROUGHT-RELATED TRAITS
DROUGHT-TOLERANT ECOTYPES
DUPLICATED GENES
PHYLOGENETICS
title_short Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
title_full Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
title_fullStr Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
title_full_unstemmed Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
title_sort Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
dc.creator.none.fl_str_mv Decena, Maria Angeles
Gálvez Rojas, Sergio
Agostini, Federico
Sancho, Ruben
Contreras Moreira, Bruno
Des Marais, David L.
Hernandez, Pilar
Catalán, Pilar
author Decena, Maria Angeles
author_facet Decena, Maria Angeles
Gálvez Rojas, Sergio
Agostini, Federico
Sancho, Ruben
Contreras Moreira, Bruno
Des Marais, David L.
Hernandez, Pilar
Catalán, Pilar
author_role author
author2 Gálvez Rojas, Sergio
Agostini, Federico
Sancho, Ruben
Contreras Moreira, Bruno
Des Marais, David L.
Hernandez, Pilar
Catalán, Pilar
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BDHN GENES
CIS-REGULATORY ELEMENTS
COMPARATIVE GENOMICS
DEHYDRIN STRUCTURE
DEHYDRIN-GENE EXPRESSION
DROUGHT-RELATED TRAITS
DROUGHT-TOLERANT ECOTYPES
DUPLICATED GENES
PHYLOGENETICS
topic BDHN GENES
CIS-REGULATORY ELEMENTS
COMPARATIVE GENOMICS
DEHYDRIN STRUCTURE
DEHYDRIN-GENE EXPRESSION
DROUGHT-RELATED TRAITS
DROUGHT-TOLERANT ECOTYPES
DUPLICATED GENES
PHYLOGENETICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachy-podium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.
Fil: Decena, Maria Angeles. Universidad de Zaragoza; España
Fil: Gálvez Rojas, Sergio. Universidad de Málaga; España
Fil: Agostini, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sancho, Ruben. Universidad de Zaragoza; España
Fil: Contreras Moreira, Bruno. Consejo Superior de Investigaciones Científicas; España
Fil: Des Marais, David L.. Massachusetts Institute of Technology; Reino Unido
Fil: Hernandez, Pilar. Consejo Superior de Investigaciones Científicas; España
Fil: Catalán, Pilar. Universidad de Zaragoza; España
description Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachy-podium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168078
Decena, Maria Angeles; Gálvez Rojas, Sergio; Agostini, Federico; Sancho, Ruben; Contreras Moreira, Bruno; et al.; Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses; MDPI; Plants; 10; 12; 12-2021; 1-29
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168078
identifier_str_mv Decena, Maria Angeles; Gálvez Rojas, Sergio; Agostini, Federico; Sancho, Ruben; Contreras Moreira, Bruno; et al.; Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses; MDPI; Plants; 10; 12; 12-2021; 1-29
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/12/2664
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10122664
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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