An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives

Autores
Hockl, Pablo Francisco; Thyssen, Sandra M; Libertun, Carlos
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method,examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.
Fil: Hockl, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Thyssen, Sandra M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Hplc
Polyamines
Benzoil Derivatives
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30848
Acceder
id CONICETDig_2400b358a6eadc73aaf1756980258f70
oai_identifier_str oai:ri.conicet.gov.ar:11336/30848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivativesHockl, Pablo FranciscoThyssen, Sandra MLibertun, CarlosHplcPolyaminesBenzoil Derivativeshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method,examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.Fil: Hockl, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Thyssen, Sandra M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaTaylor2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30848Libertun, Carlos; Thyssen, Sandra M; Hockl, Pablo Francisco; An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives; Taylor; Journal of Liquid Chromatography and Related Technologies; 23; 5; 5-2000; 693-7031082-6076CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1081/JLC-100101482info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1081/JLC-100101482?journalCode=ljlc19&quickLinkVolume=23&quickLinkIssue=5&quickLinkPage=693&quickLinkYear=2000&selectedTab=citation&volume=23info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:42Zoai:ri.conicet.gov.ar:11336/30848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:42.744CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
title An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
spellingShingle An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
Hockl, Pablo Francisco
Hplc
Polyamines
Benzoil Derivatives
title_short An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
title_full An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
title_fullStr An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
title_full_unstemmed An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
title_sort An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives
dc.creator.none.fl_str_mv Hockl, Pablo Francisco
Thyssen, Sandra M
Libertun, Carlos
author Hockl, Pablo Francisco
author_facet Hockl, Pablo Francisco
Thyssen, Sandra M
Libertun, Carlos
author_role author
author2 Thyssen, Sandra M
Libertun, Carlos
author2_role author
author
dc.subject.none.fl_str_mv Hplc
Polyamines
Benzoil Derivatives
topic Hplc
Polyamines
Benzoil Derivatives
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method,examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.
Fil: Hockl, Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Thyssen, Sandra M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method,examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30848
Libertun, Carlos; Thyssen, Sandra M; Hockl, Pablo Francisco; An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives; Taylor; Journal of Liquid Chromatography and Related Technologies; 23; 5; 5-2000; 693-703
1082-6076
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30848
identifier_str_mv Libertun, Carlos; Thyssen, Sandra M; Hockl, Pablo Francisco; An improved method for the identification and cuantitation of polyamines and related compounds as benzoilated derivatives; Taylor; Journal of Liquid Chromatography and Related Technologies; 23; 5; 5-2000; 693-703
1082-6076
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1081/JLC-100101482
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1081/JLC-100101482?journalCode=ljlc19&quickLinkVolume=23&quickLinkIssue=5&quickLinkPage=693&quickLinkYear=2000&selectedTab=citation&volume=23
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor
publisher.none.fl_str_mv Taylor
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082714968522752
score 13.22299

Publicaciones similares