Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America

Autores
Krivokapich, Silvio Jesús; Pozio, Edoardo; Gatti, Graciana M.; González Prous, Cinthia L.; Ribicich, Miriam Mabel; Marucci, Gianluca; La Rosa, Giuseppe; Confalonieri, Viviana Andrea
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp.
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Pozio, Edoardo. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: Gatti, Graciana M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: González Prous, Cinthia L.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; Argentina
Fil: Marucci, Gianluca. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: La Rosa, Giuseppe. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
Argentina
Nematoda
New Species
Puma Concolor
Ribosomal Dna
Taxonomy
Trichinella Patagoniensis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68212
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68212
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South AmericaKrivokapich, Silvio JesúsPozio, EdoardoGatti, Graciana M.González Prous, Cinthia L.Ribicich, Miriam MabelMarucci, GianlucaLa Rosa, GiuseppeConfalonieri, Viviana AndreaArgentinaNematodaNew SpeciesPuma ConcolorRibosomal DnaTaxonomyTrichinella Patagoniensishttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp.Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; ArgentinaFil: Pozio, Edoardo. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; ItaliaFil: Gatti, Graciana M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; ArgentinaFil: González Prous, Cinthia L.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; ArgentinaFil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Marucci, Gianluca. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; ItaliaFil: La Rosa, Giuseppe. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; ItaliaFil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaElsevier2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68212Krivokapich, Silvio Jesús; Pozio, Edoardo; Gatti, Graciana M.; González Prous, Cinthia L.; Ribicich, Miriam Mabel; et al.; Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America; Elsevier; International Journal for Parasitology; 42; 10; 9-2012; 903-9100020-7519CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2012.07.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751912001932info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:08Zoai:ri.conicet.gov.ar:11336/68212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:08.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
spellingShingle Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
Krivokapich, Silvio Jesús
Argentina
Nematoda
New Species
Puma Concolor
Ribosomal Dna
Taxonomy
Trichinella Patagoniensis
title_short Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_full Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_fullStr Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_full_unstemmed Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
title_sort Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
dc.creator.none.fl_str_mv Krivokapich, Silvio Jesús
Pozio, Edoardo
Gatti, Graciana M.
González Prous, Cinthia L.
Ribicich, Miriam Mabel
Marucci, Gianluca
La Rosa, Giuseppe
Confalonieri, Viviana Andrea
author Krivokapich, Silvio Jesús
author_facet Krivokapich, Silvio Jesús
Pozio, Edoardo
Gatti, Graciana M.
González Prous, Cinthia L.
Ribicich, Miriam Mabel
Marucci, Gianluca
La Rosa, Giuseppe
Confalonieri, Viviana Andrea
author_role author
author2 Pozio, Edoardo
Gatti, Graciana M.
González Prous, Cinthia L.
Ribicich, Miriam Mabel
Marucci, Gianluca
La Rosa, Giuseppe
Confalonieri, Viviana Andrea
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentina
Nematoda
New Species
Puma Concolor
Ribosomal Dna
Taxonomy
Trichinella Patagoniensis
topic Argentina
Nematoda
New Species
Puma Concolor
Ribosomal Dna
Taxonomy
Trichinella Patagoniensis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp.
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Pozio, Edoardo. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: Gatti, Graciana M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: González Prous, Cinthia L.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; Argentina
Fil: Marucci, Gianluca. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: La Rosa, Giuseppe. Istituto Superiore di Sanità. Department of Infectious, Parasitic and Immunomediated Diseases; Italia
Fil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68212
Krivokapich, Silvio Jesús; Pozio, Edoardo; Gatti, Graciana M.; González Prous, Cinthia L.; Ribicich, Miriam Mabel; et al.; Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America; Elsevier; International Journal for Parasitology; 42; 10; 9-2012; 903-910
0020-7519
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68212
identifier_str_mv Krivokapich, Silvio Jesús; Pozio, Edoardo; Gatti, Graciana M.; González Prous, Cinthia L.; Ribicich, Miriam Mabel; et al.; Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America; Elsevier; International Journal for Parasitology; 42; 10; 9-2012; 903-910
0020-7519
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2012.07.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751912001932
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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