Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument

Autores
Calderón Fernández, Gustavo Mario; Moriconi, Debora Elizabeth; Dulbecco, Andrea Belen; Juarez, Marta Patricia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The insect integument, formed by the cuticle and the underlying epidermis, is essential for insect fitness, regulation of lipid biosynthesis and storage, insect growth and feeding, together with development progress. Its participation in insecticide resistance has also been outlined. Triatoma infestans Klug is one of the major vectors of Chagas disease in South America; however, genomic data is scarce. In this study, we performed a transcriptome analysis of the nymph integument in order to identify which genes are expressed and their putative role. Using the 454 GS-FLX sequencing platform, we obtained approximately 144620 reads from the integument tissue. These reads were assembled into 6495 isotigs and 8504 singletons. Based on BLAST similarity searches, about 8000 transcripts were annotated with known genes, conserved domains, and/or Gene Ontology (GO) terms. The most abundant transcripts corresponded to transcription factors and nucleic acid metabolism, membrane receptors, cell signaling, and proteins related to cytoskeleton, transport and cell energy processes, among others. More than 10% of the transcripts encoded proteins putatively involved in the metabolism of fatty acids and related components (fatty acid synthases, elongases, desaturases, fatty alcohol reductases), structural integument proteins, and the insecticide detoxification system (among them, cytochrome P450s, esterases and glutathione transferases). Real time qPCR assays were used to investigate their putative participation in the resistance mechanism. This preliminary study is the first transcriptome analysis of a triatomine integument, and together with prior biochemical information, will help further understand the role of the integument in a wide array of mechanisms
Fil: Calderón Fernández, Gustavo Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Moriconi, Debora Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Dulbecco, Andrea Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Materia
Transcriptome
Integument
Insecticide Resistance
Lipids
Cuticle Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48973
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48973
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) IntegumentCalderón Fernández, Gustavo MarioMoriconi, Debora ElizabethDulbecco, Andrea BelenJuarez, Marta PatriciaTranscriptomeIntegumentInsecticide ResistanceLipidsCuticle Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insect integument, formed by the cuticle and the underlying epidermis, is essential for insect fitness, regulation of lipid biosynthesis and storage, insect growth and feeding, together with development progress. Its participation in insecticide resistance has also been outlined. Triatoma infestans Klug is one of the major vectors of Chagas disease in South America; however, genomic data is scarce. In this study, we performed a transcriptome analysis of the nymph integument in order to identify which genes are expressed and their putative role. Using the 454 GS-FLX sequencing platform, we obtained approximately 144620 reads from the integument tissue. These reads were assembled into 6495 isotigs and 8504 singletons. Based on BLAST similarity searches, about 8000 transcripts were annotated with known genes, conserved domains, and/or Gene Ontology (GO) terms. The most abundant transcripts corresponded to transcription factors and nucleic acid metabolism, membrane receptors, cell signaling, and proteins related to cytoskeleton, transport and cell energy processes, among others. More than 10% of the transcripts encoded proteins putatively involved in the metabolism of fatty acids and related components (fatty acid synthases, elongases, desaturases, fatty alcohol reductases), structural integument proteins, and the insecticide detoxification system (among them, cytochrome P450s, esterases and glutathione transferases). Real time qPCR assays were used to investigate their putative participation in the resistance mechanism. This preliminary study is the first transcriptome analysis of a triatomine integument, and together with prior biochemical information, will help further understand the role of the integument in a wide array of mechanismsFil: Calderón Fernández, Gustavo Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Moriconi, Debora Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Dulbecco, Andrea Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaEntomological Society of America2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48973Calderón Fernández, Gustavo Mario; Moriconi, Debora Elizabeth; Dulbecco, Andrea Belen; Juarez, Marta Patricia; Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument; Entomological Society of America; Journal of Medical Entomology; 54; 6; 9-2017; 1531-15420022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jme/tjx151info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/54/6/1531/4243555info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:42Zoai:ri.conicet.gov.ar:11336/48973instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:43.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
title Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
spellingShingle Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
Calderón Fernández, Gustavo Mario
Transcriptome
Integument
Insecticide Resistance
Lipids
Cuticle Proteins
title_short Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
title_full Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
title_fullStr Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
title_full_unstemmed Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
title_sort Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument
dc.creator.none.fl_str_mv Calderón Fernández, Gustavo Mario
Moriconi, Debora Elizabeth
Dulbecco, Andrea Belen
Juarez, Marta Patricia
author Calderón Fernández, Gustavo Mario
author_facet Calderón Fernández, Gustavo Mario
Moriconi, Debora Elizabeth
Dulbecco, Andrea Belen
Juarez, Marta Patricia
author_role author
author2 Moriconi, Debora Elizabeth
Dulbecco, Andrea Belen
Juarez, Marta Patricia
author2_role author
author
author
dc.subject.none.fl_str_mv Transcriptome
Integument
Insecticide Resistance
Lipids
Cuticle Proteins
topic Transcriptome
Integument
Insecticide Resistance
Lipids
Cuticle Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The insect integument, formed by the cuticle and the underlying epidermis, is essential for insect fitness, regulation of lipid biosynthesis and storage, insect growth and feeding, together with development progress. Its participation in insecticide resistance has also been outlined. Triatoma infestans Klug is one of the major vectors of Chagas disease in South America; however, genomic data is scarce. In this study, we performed a transcriptome analysis of the nymph integument in order to identify which genes are expressed and their putative role. Using the 454 GS-FLX sequencing platform, we obtained approximately 144620 reads from the integument tissue. These reads were assembled into 6495 isotigs and 8504 singletons. Based on BLAST similarity searches, about 8000 transcripts were annotated with known genes, conserved domains, and/or Gene Ontology (GO) terms. The most abundant transcripts corresponded to transcription factors and nucleic acid metabolism, membrane receptors, cell signaling, and proteins related to cytoskeleton, transport and cell energy processes, among others. More than 10% of the transcripts encoded proteins putatively involved in the metabolism of fatty acids and related components (fatty acid synthases, elongases, desaturases, fatty alcohol reductases), structural integument proteins, and the insecticide detoxification system (among them, cytochrome P450s, esterases and glutathione transferases). Real time qPCR assays were used to investigate their putative participation in the resistance mechanism. This preliminary study is the first transcriptome analysis of a triatomine integument, and together with prior biochemical information, will help further understand the role of the integument in a wide array of mechanisms
Fil: Calderón Fernández, Gustavo Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Moriconi, Debora Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Dulbecco, Andrea Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
description The insect integument, formed by the cuticle and the underlying epidermis, is essential for insect fitness, regulation of lipid biosynthesis and storage, insect growth and feeding, together with development progress. Its participation in insecticide resistance has also been outlined. Triatoma infestans Klug is one of the major vectors of Chagas disease in South America; however, genomic data is scarce. In this study, we performed a transcriptome analysis of the nymph integument in order to identify which genes are expressed and their putative role. Using the 454 GS-FLX sequencing platform, we obtained approximately 144620 reads from the integument tissue. These reads were assembled into 6495 isotigs and 8504 singletons. Based on BLAST similarity searches, about 8000 transcripts were annotated with known genes, conserved domains, and/or Gene Ontology (GO) terms. The most abundant transcripts corresponded to transcription factors and nucleic acid metabolism, membrane receptors, cell signaling, and proteins related to cytoskeleton, transport and cell energy processes, among others. More than 10% of the transcripts encoded proteins putatively involved in the metabolism of fatty acids and related components (fatty acid synthases, elongases, desaturases, fatty alcohol reductases), structural integument proteins, and the insecticide detoxification system (among them, cytochrome P450s, esterases and glutathione transferases). Real time qPCR assays were used to investigate their putative participation in the resistance mechanism. This preliminary study is the first transcriptome analysis of a triatomine integument, and together with prior biochemical information, will help further understand the role of the integument in a wide array of mechanisms
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48973
Calderón Fernández, Gustavo Mario; Moriconi, Debora Elizabeth; Dulbecco, Andrea Belen; Juarez, Marta Patricia; Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument; Entomological Society of America; Journal of Medical Entomology; 54; 6; 9-2017; 1531-1542
0022-2585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48973
identifier_str_mv Calderón Fernández, Gustavo Mario; Moriconi, Debora Elizabeth; Dulbecco, Andrea Belen; Juarez, Marta Patricia; Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument; Entomological Society of America; Journal of Medical Entomology; 54; 6; 9-2017; 1531-1542
0022-2585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jme/tjx151
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/54/6/1531/4243555
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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