Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
- Autores
- Viera, Marisa Rosana
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
17° Congreso Internacional de Metalurgia y Materiales
Copiapó
Chile
Sociedad Chilena de Metalurgia y Materiales
Asociación Argentina de Materiales - Materia
-
SRB
1010 CARBON STEEL
SEM
EIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/199001
Ver los metadatos del registro completo
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Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopyViera, Marisa RosanaSRB1010 CARBON STEELSEMEIShttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina17° Congreso Internacional de Metalurgia y MaterialesCopiapóChileSociedad Chilena de Metalurgia y MaterialesAsociación Argentina de MaterialesUniversidad de Atacama2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199001Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:39Zoai:ri.conicet.gov.ar:11336/199001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:39.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
title |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
spellingShingle |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy Viera, Marisa Rosana SRB 1010 CARBON STEEL SEM EIS |
title_short |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
title_full |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
title_fullStr |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
title_full_unstemmed |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
title_sort |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy |
dc.creator.none.fl_str_mv |
Viera, Marisa Rosana |
author |
Viera, Marisa Rosana |
author_facet |
Viera, Marisa Rosana |
author_role |
author |
dc.subject.none.fl_str_mv |
SRB 1010 CARBON STEEL SEM EIS |
topic |
SRB 1010 CARBON STEEL SEM EIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.5 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time. Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina 17° Congreso Internacional de Metalurgia y Materiales Copiapó Chile Sociedad Chilena de Metalurgia y Materiales Asociación Argentina de Materiales |
description |
Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/199001 Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/199001 |
identifier_str_mv |
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Universidad de Atacama |
publisher.none.fl_str_mv |
Universidad de Atacama |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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