Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy

Autores
Viera, Marisa Rosana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
17° Congreso Internacional de Metalurgia y Materiales
Copiapó
Chile
Sociedad Chilena de Metalurgia y Materiales
Asociación Argentina de Materiales
Materia
SRB
1010 CARBON STEEL
SEM
EIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199001

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spelling Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopyViera, Marisa RosanaSRB1010 CARBON STEELSEMEIShttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina17° Congreso Internacional de Metalurgia y MaterialesCopiapóChileSociedad Chilena de Metalurgia y MaterialesAsociación Argentina de MaterialesUniversidad de Atacama2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199001Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:39Zoai:ri.conicet.gov.ar:11336/199001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:39.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
title Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
spellingShingle Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
Viera, Marisa Rosana
SRB
1010 CARBON STEEL
SEM
EIS
title_short Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
title_full Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
title_fullStr Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
title_full_unstemmed Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
title_sort Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy
dc.creator.none.fl_str_mv Viera, Marisa Rosana
author Viera, Marisa Rosana
author_facet Viera, Marisa Rosana
author_role author
dc.subject.none.fl_str_mv SRB
1010 CARBON STEEL
SEM
EIS
topic SRB
1010 CARBON STEEL
SEM
EIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
17° Congreso Internacional de Metalurgia y Materiales
Copiapó
Chile
Sociedad Chilena de Metalurgia y Materiales
Asociación Argentina de Materiales
description Biofilms cause various problems such as medical infections, fouling of water cooling system, product contamination, and microbiologically influenced corrosion (MIC)[1]. MIC accounts for as much as 20% of all forms of corrosion, amounting to billions of dollars in losses each year[2]. Among aerobic and anaerobic bacteria related to MIC, sulfate reducing bacteria (SRB) are most often blamed [3]. The ubiquity of these bacteria leads to a variety of impressive industrial, economic and ecological effects because of their proneness to generate large quantities of H2S. SRB are the main reason to cause the MIC by accelerating corrosion rate, inducing stress corrosion and pitting corrosion [4-6]. The aim of this study was to evaluate the corrosion associated with the formation of SRB biofilms on carbon steel surfaces. The bacterial strain used in the experiments was Desulfovibrio vulgaris cultured in Postgate´C. Carbon steel coupons of SAE 1010 were placed in the cultures for biofilm development during 1 and 7 days. Then, the coupons were extracted and bacterial adherence and biofilm formation were measured by viable bacteria counts, epifluorescence microscopy and by the crystal violet assay. Surface attack of carbon steel and biofilm morphology were analyzed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Studies carried out allowed correlating the biofilm formation (Figure 1) with the different degree of attack suffered by the SAE 1010 carbon steel coupons. The impedance spectra (Figure 2) show that the charge transfer resistance increases with the time.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199001
Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199001
identifier_str_mv Biofilm formation and corrosion behaviour of 1010 carbon steel in SRB cultures by microscopic techniques and electrochemical impedance spectroscopy; 17° Congreso Internacional de Metalurgia y Materiales; Copiapó; Chile; 2017; 1-7
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Atacama
publisher.none.fl_str_mv Universidad de Atacama
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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