Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree

Autores
Molina, Lucia; Rajchenberg, Mario; Pildain, María Belén; Aime, Mary Catherine
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fungal communities inhabiting leaves are key players in ecosystem processes but remain largely unexplored in Southern Hemisphere temperate forests. We characterized the foliar mycobiota of Nothofagus pumilio, a dominant deciduous tree in Patagonian forests, using ITS1 metabarcoding across seasons and tree health conditions. We detected 426 fungal taxa, including a 40-Amplicon Sequence Variant (ASV) core mycobiome persisting year-round. Fungal richness and biomass increased significantly in autumn, coinciding with leaf senescence, and community composition shifted markedly between seasons. Spring leaves were enriched in pathogens and basidiomycetous yeasts, while autumn leaves hosted more saprotrophs, ascomycetous yeasts, and lichen-associated fungi. Tree health had limited influence on overall community structure, but symptomatic trees showed higher ASV richness and specific indicator taxa, including the pathogen Trichosporiella multisporum and members of the Taphrinaceae and Saccotheciaceae families. Despite taxonomic turnover, ecological guilds remained relatively stable, suggesting functional redundancy. These findings reveal a seasonal successional trajectory in the foliar mycobiota of N. pumilio, from early-colonizing endophytes in spring to diverse decomposer assemblages in autumn. This study provides the first high-throughput insight into the structure and dynamics of foliar fungal communities in Southern Hemisphere temperate forests, offering a baseline for understanding microbial roles in forest health and resilience.
Fil: Molina, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Rajchenberg, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Aime, Mary Catherine. Purdue University; Estados Unidos
Materia
PHYLLOSPHERE FUNGI
ENDOPHYTES
HIGH-THROUGHPUT SEQUENCING
FUNCTIONAL GUILDS
TEMPERATE NOTHOFAGUS FORESTS
TAPHRINA ENTOMOSPORA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276961
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276961
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone TreeMolina, LuciaRajchenberg, MarioPildain, María BelénAime, Mary CatherinePHYLLOSPHERE FUNGIENDOPHYTESHIGH-THROUGHPUT SEQUENCINGFUNCTIONAL GUILDSTEMPERATE NOTHOFAGUS FORESTSTAPHRINA ENTOMOSPORAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fungal communities inhabiting leaves are key players in ecosystem processes but remain largely unexplored in Southern Hemisphere temperate forests. We characterized the foliar mycobiota of Nothofagus pumilio, a dominant deciduous tree in Patagonian forests, using ITS1 metabarcoding across seasons and tree health conditions. We detected 426 fungal taxa, including a 40-Amplicon Sequence Variant (ASV) core mycobiome persisting year-round. Fungal richness and biomass increased significantly in autumn, coinciding with leaf senescence, and community composition shifted markedly between seasons. Spring leaves were enriched in pathogens and basidiomycetous yeasts, while autumn leaves hosted more saprotrophs, ascomycetous yeasts, and lichen-associated fungi. Tree health had limited influence on overall community structure, but symptomatic trees showed higher ASV richness and specific indicator taxa, including the pathogen Trichosporiella multisporum and members of the Taphrinaceae and Saccotheciaceae families. Despite taxonomic turnover, ecological guilds remained relatively stable, suggesting functional redundancy. These findings reveal a seasonal successional trajectory in the foliar mycobiota of N. pumilio, from early-colonizing endophytes in spring to diverse decomposer assemblages in autumn. This study provides the first high-throughput insight into the structure and dynamics of foliar fungal communities in Southern Hemisphere temperate forests, offering a baseline for understanding microbial roles in forest health and resilience.Fil: Molina, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; ArgentinaFil: Rajchenberg, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; ArgentinaFil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Aime, Mary Catherine. Purdue University; Estados UnidosMultidisciplinary Digital Publishing Institute2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276961Molina, Lucia; Rajchenberg, Mario; Pildain, María Belén; Aime, Mary Catherine; Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 11; 11; 11-2025; 1-242309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/11/11/795info:eu-repo/semantics/altIdentifier/doi/10.3390/jof11110795info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:22:50Zoai:ri.conicet.gov.ar:11336/276961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:22:51.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
title Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
spellingShingle Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
Molina, Lucia
PHYLLOSPHERE FUNGI
ENDOPHYTES
HIGH-THROUGHPUT SEQUENCING
FUNCTIONAL GUILDS
TEMPERATE NOTHOFAGUS FORESTS
TAPHRINA ENTOMOSPORA
title_short Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
title_full Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
title_fullStr Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
title_full_unstemmed Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
title_sort Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree
dc.creator.none.fl_str_mv Molina, Lucia
Rajchenberg, Mario
Pildain, María Belén
Aime, Mary Catherine
author Molina, Lucia
author_facet Molina, Lucia
Rajchenberg, Mario
Pildain, María Belén
Aime, Mary Catherine
author_role author
author2 Rajchenberg, Mario
Pildain, María Belén
Aime, Mary Catherine
author2_role author
author
author
dc.subject.none.fl_str_mv PHYLLOSPHERE FUNGI
ENDOPHYTES
HIGH-THROUGHPUT SEQUENCING
FUNCTIONAL GUILDS
TEMPERATE NOTHOFAGUS FORESTS
TAPHRINA ENTOMOSPORA
topic PHYLLOSPHERE FUNGI
ENDOPHYTES
HIGH-THROUGHPUT SEQUENCING
FUNCTIONAL GUILDS
TEMPERATE NOTHOFAGUS FORESTS
TAPHRINA ENTOMOSPORA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fungal communities inhabiting leaves are key players in ecosystem processes but remain largely unexplored in Southern Hemisphere temperate forests. We characterized the foliar mycobiota of Nothofagus pumilio, a dominant deciduous tree in Patagonian forests, using ITS1 metabarcoding across seasons and tree health conditions. We detected 426 fungal taxa, including a 40-Amplicon Sequence Variant (ASV) core mycobiome persisting year-round. Fungal richness and biomass increased significantly in autumn, coinciding with leaf senescence, and community composition shifted markedly between seasons. Spring leaves were enriched in pathogens and basidiomycetous yeasts, while autumn leaves hosted more saprotrophs, ascomycetous yeasts, and lichen-associated fungi. Tree health had limited influence on overall community structure, but symptomatic trees showed higher ASV richness and specific indicator taxa, including the pathogen Trichosporiella multisporum and members of the Taphrinaceae and Saccotheciaceae families. Despite taxonomic turnover, ecological guilds remained relatively stable, suggesting functional redundancy. These findings reveal a seasonal successional trajectory in the foliar mycobiota of N. pumilio, from early-colonizing endophytes in spring to diverse decomposer assemblages in autumn. This study provides the first high-throughput insight into the structure and dynamics of foliar fungal communities in Southern Hemisphere temperate forests, offering a baseline for understanding microbial roles in forest health and resilience.
Fil: Molina, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Rajchenberg, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Aime, Mary Catherine. Purdue University; Estados Unidos
description Fungal communities inhabiting leaves are key players in ecosystem processes but remain largely unexplored in Southern Hemisphere temperate forests. We characterized the foliar mycobiota of Nothofagus pumilio, a dominant deciduous tree in Patagonian forests, using ITS1 metabarcoding across seasons and tree health conditions. We detected 426 fungal taxa, including a 40-Amplicon Sequence Variant (ASV) core mycobiome persisting year-round. Fungal richness and biomass increased significantly in autumn, coinciding with leaf senescence, and community composition shifted markedly between seasons. Spring leaves were enriched in pathogens and basidiomycetous yeasts, while autumn leaves hosted more saprotrophs, ascomycetous yeasts, and lichen-associated fungi. Tree health had limited influence on overall community structure, but symptomatic trees showed higher ASV richness and specific indicator taxa, including the pathogen Trichosporiella multisporum and members of the Taphrinaceae and Saccotheciaceae families. Despite taxonomic turnover, ecological guilds remained relatively stable, suggesting functional redundancy. These findings reveal a seasonal successional trajectory in the foliar mycobiota of N. pumilio, from early-colonizing endophytes in spring to diverse decomposer assemblages in autumn. This study provides the first high-throughput insight into the structure and dynamics of foliar fungal communities in Southern Hemisphere temperate forests, offering a baseline for understanding microbial roles in forest health and resilience.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276961
Molina, Lucia; Rajchenberg, Mario; Pildain, María Belén; Aime, Mary Catherine; Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 11; 11; 11-2025; 1-24
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276961
identifier_str_mv Molina, Lucia; Rajchenberg, Mario; Pildain, María Belén; Aime, Mary Catherine; Seasonal Turnover and Functional Structure of the Foliar Mycobiota in a Gondwanan Temperate Forest Keystone Tree; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 11; 11; 11-2025; 1-24
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/11/11/795
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof11110795
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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