Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8

Autores
Fessia, Alumine Soledad; Sartori, Melina Victoria; Orlando, Julieta; Barros, Germán Gustavo; Nesci, Andrea Verónica
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, the genomes of B. subtilis EM-A7 and B. velezensis EM-A8 were sequenced andannotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomicDNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carriedout by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classifiedtaxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through adDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EMA7,and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findingsenhance our understanding of the two strains’ potential as biocontrol agents to manage disease inmaize.
Fil: Fessia, Alumine Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Orlando, Julieta. Universidad de Chile; Chile
Fil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina
Materia
Draft genome sequencing
Biofilm
Bacillus
Phyllosphere
Biocontrol agent
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238758
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/238758
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8Fessia, Alumine SoledadSartori, Melina VictoriaOrlando, JulietaBarros, Germán GustavoNesci, Andrea VerónicaDraft genome sequencingBiofilmBacillusPhyllosphereBiocontrol agenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study, the genomes of B. subtilis EM-A7 and B. velezensis EM-A8 were sequenced andannotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomicDNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carriedout by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classifiedtaxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through adDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EMA7,and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findingsenhance our understanding of the two strains’ potential as biocontrol agents to manage disease inmaize.Fil: Fessia, Alumine Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Orlando, Julieta. Universidad de Chile; ChileFil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; ArgentinaElsevier2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238758Fessia, Alumine Soledad; Sartori, Melina Victoria; Orlando, Julieta; Barros, Germán Gustavo; Nesci, Andrea Verónica; Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8; Elsevier; Heliyon; 10; 12; 6-2024; 1-112405-8440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2405844024086389info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2024.e32607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:35:28Zoai:ri.conicet.gov.ar:11336/238758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:29.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
title Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
spellingShingle Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
Fessia, Alumine Soledad
Draft genome sequencing
Biofilm
Bacillus
Phyllosphere
Biocontrol agent
title_short Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
title_full Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
title_fullStr Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
title_full_unstemmed Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
title_sort Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8
dc.creator.none.fl_str_mv Fessia, Alumine Soledad
Sartori, Melina Victoria
Orlando, Julieta
Barros, Germán Gustavo
Nesci, Andrea Verónica
author Fessia, Alumine Soledad
author_facet Fessia, Alumine Soledad
Sartori, Melina Victoria
Orlando, Julieta
Barros, Germán Gustavo
Nesci, Andrea Verónica
author_role author
author2 Sartori, Melina Victoria
Orlando, Julieta
Barros, Germán Gustavo
Nesci, Andrea Verónica
author2_role author
author
author
author
dc.subject.none.fl_str_mv Draft genome sequencing
Biofilm
Bacillus
Phyllosphere
Biocontrol agent
topic Draft genome sequencing
Biofilm
Bacillus
Phyllosphere
Biocontrol agent
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study, the genomes of B. subtilis EM-A7 and B. velezensis EM-A8 were sequenced andannotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomicDNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carriedout by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classifiedtaxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through adDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EMA7,and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findingsenhance our understanding of the two strains’ potential as biocontrol agents to manage disease inmaize.
Fil: Fessia, Alumine Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Orlando, Julieta. Universidad de Chile; Chile
Fil: Barros, Germán Gustavo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina
description In the present study, the genomes of B. subtilis EM-A7 and B. velezensis EM-A8 were sequenced andannotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomicDNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carriedout by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classifiedtaxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through adDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EMA7,and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findingsenhance our understanding of the two strains’ potential as biocontrol agents to manage disease inmaize.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238758
Fessia, Alumine Soledad; Sartori, Melina Victoria; Orlando, Julieta; Barros, Germán Gustavo; Nesci, Andrea Verónica; Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8; Elsevier; Heliyon; 10; 12; 6-2024; 1-11
2405-8440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238758
identifier_str_mv Fessia, Alumine Soledad; Sartori, Melina Victoria; Orlando, Julieta; Barros, Germán Gustavo; Nesci, Andrea Verónica; Draft genome sequences of two biocontrol agents isolated from the maize phyllosphere: Bacillus subtilis strain EM-A7 and Bacillus velezensis strain EM-A8; Elsevier; Heliyon; 10; 12; 6-2024; 1-11
2405-8440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2405844024086389
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2024.e32607
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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