In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound

Autores
Rizzitelli, Silvia; Giustetto, Pierangela; Boffa, Cinzia; Delli Castelli, Daniela; Cutrin, Juan Carlos; Aime, Silvio; Terreno, Enzo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The work aimed at developing a MRI-guided protocol for the visualization of the release of material entrapped in liposomes stimulated by the local application of pulsed low-intensity non-focused ultrasound (pLINFU). The task was achieved by formulating liposomes filled up with the clinically approved paramagnetic agent gadoteridol, because the release of the agent from the nanovesicles is accompanied by a significant MRI signal enhancement. The protocol was validated in vivo on mice-bearing subcutaneous syngeneic B16 melanoma and i.v. injected with the paramagnetic liposomes. Upon exposing tumor to pLINFU (3 MHz, insonation time 2 min, duty cycle 50%) few minutes after liposomes injection, a signal enhancement of ca. 35% was detected. The effective release of the agent was confirmed by the strong enhancement measured in kidneys calyx and bladder due to the rapid renal excretion of the agent released in the tumor.From the Clinical EditorIn this paper, a pulsed low-intensity non-focused ultrasound-based technique was used to release a paramagnetic MRI contrast agent from liposomes, demonstrating the feasibility of this triggered release system in a mouse melanoma model for future research applications.
Fil: Rizzitelli, Silvia. Universitã â  Di Torino; Italia
Fil: Giustetto, Pierangela. Universitã â  Di Torino; Italia
Fil: Boffa, Cinzia. Universitã â  Di Torino; Italia
Fil: Delli Castelli, Daniela. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Aime, Silvio. Universitã â  Di Torino; Italia
Fil: Terreno, Enzo. Universitã â  Di Torino; Italia
Materia
Drug Release
Theranosis
Liposomes
Ultrasound
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7867
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7867
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasoundRizzitelli, SilviaGiustetto, PierangelaBoffa, CinziaDelli Castelli, DanielaCutrin, Juan CarlosAime, SilvioTerreno, EnzoDrug ReleaseTheranosisLiposomesUltrasoundhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The work aimed at developing a MRI-guided protocol for the visualization of the release of material entrapped in liposomes stimulated by the local application of pulsed low-intensity non-focused ultrasound (pLINFU). The task was achieved by formulating liposomes filled up with the clinically approved paramagnetic agent gadoteridol, because the release of the agent from the nanovesicles is accompanied by a significant MRI signal enhancement. The protocol was validated in vivo on mice-bearing subcutaneous syngeneic B16 melanoma and i.v. injected with the paramagnetic liposomes. Upon exposing tumor to pLINFU (3 MHz, insonation time 2 min, duty cycle 50%) few minutes after liposomes injection, a signal enhancement of ca. 35% was detected. The effective release of the agent was confirmed by the strong enhancement measured in kidneys calyx and bladder due to the rapid renal excretion of the agent released in the tumor.From the Clinical EditorIn this paper, a pulsed low-intensity non-focused ultrasound-based technique was used to release a paramagnetic MRI contrast agent from liposomes, demonstrating the feasibility of this triggered release system in a mouse melanoma model for future research applications.Fil: Rizzitelli, Silvia. Universitã â  Di Torino; ItaliaFil: Giustetto, Pierangela. Universitã â  Di Torino; ItaliaFil: Boffa, Cinzia. Universitã â  Di Torino; ItaliaFil: Delli Castelli, Daniela. Universitã â  Di Torino; ItaliaFil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); ArgentinaFil: Aime, Silvio. Universitã â  Di Torino; ItaliaFil: Terreno, Enzo. Universitã â  Di Torino; ItaliaElsevier Science2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7867Rizzitelli, Silvia; Giustetto, Pierangela; Boffa, Cinzia; Delli Castelli, Daniela; Cutrin, Juan Carlos; et al.; In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound; Elsevier Science; Nanomedicine-nanotechnology Biology And Medicine; 10; 5; 3-2014; 901-9041549-9634enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1549963414001300info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nano.2014.03.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:58Zoai:ri.conicet.gov.ar:11336/7867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:58.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
title In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
spellingShingle In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
Rizzitelli, Silvia
Drug Release
Theranosis
Liposomes
Ultrasound
title_short In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
title_full In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
title_fullStr In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
title_full_unstemmed In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
title_sort In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
dc.creator.none.fl_str_mv Rizzitelli, Silvia
Giustetto, Pierangela
Boffa, Cinzia
Delli Castelli, Daniela
Cutrin, Juan Carlos
Aime, Silvio
Terreno, Enzo
author Rizzitelli, Silvia
author_facet Rizzitelli, Silvia
Giustetto, Pierangela
Boffa, Cinzia
Delli Castelli, Daniela
Cutrin, Juan Carlos
Aime, Silvio
Terreno, Enzo
author_role author
author2 Giustetto, Pierangela
Boffa, Cinzia
Delli Castelli, Daniela
Cutrin, Juan Carlos
Aime, Silvio
Terreno, Enzo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Drug Release
Theranosis
Liposomes
Ultrasound
topic Drug Release
Theranosis
Liposomes
Ultrasound
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The work aimed at developing a MRI-guided protocol for the visualization of the release of material entrapped in liposomes stimulated by the local application of pulsed low-intensity non-focused ultrasound (pLINFU). The task was achieved by formulating liposomes filled up with the clinically approved paramagnetic agent gadoteridol, because the release of the agent from the nanovesicles is accompanied by a significant MRI signal enhancement. The protocol was validated in vivo on mice-bearing subcutaneous syngeneic B16 melanoma and i.v. injected with the paramagnetic liposomes. Upon exposing tumor to pLINFU (3 MHz, insonation time 2 min, duty cycle 50%) few minutes after liposomes injection, a signal enhancement of ca. 35% was detected. The effective release of the agent was confirmed by the strong enhancement measured in kidneys calyx and bladder due to the rapid renal excretion of the agent released in the tumor.From the Clinical EditorIn this paper, a pulsed low-intensity non-focused ultrasound-based technique was used to release a paramagnetic MRI contrast agent from liposomes, demonstrating the feasibility of this triggered release system in a mouse melanoma model for future research applications.
Fil: Rizzitelli, Silvia. Universitã â  Di Torino; Italia
Fil: Giustetto, Pierangela. Universitã â  Di Torino; Italia
Fil: Boffa, Cinzia. Universitã â  Di Torino; Italia
Fil: Delli Castelli, Daniela. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Aime, Silvio. Universitã â  Di Torino; Italia
Fil: Terreno, Enzo. Universitã â  Di Torino; Italia
description The work aimed at developing a MRI-guided protocol for the visualization of the release of material entrapped in liposomes stimulated by the local application of pulsed low-intensity non-focused ultrasound (pLINFU). The task was achieved by formulating liposomes filled up with the clinically approved paramagnetic agent gadoteridol, because the release of the agent from the nanovesicles is accompanied by a significant MRI signal enhancement. The protocol was validated in vivo on mice-bearing subcutaneous syngeneic B16 melanoma and i.v. injected with the paramagnetic liposomes. Upon exposing tumor to pLINFU (3 MHz, insonation time 2 min, duty cycle 50%) few minutes after liposomes injection, a signal enhancement of ca. 35% was detected. The effective release of the agent was confirmed by the strong enhancement measured in kidneys calyx and bladder due to the rapid renal excretion of the agent released in the tumor.From the Clinical EditorIn this paper, a pulsed low-intensity non-focused ultrasound-based technique was used to release a paramagnetic MRI contrast agent from liposomes, demonstrating the feasibility of this triggered release system in a mouse melanoma model for future research applications.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7867
Rizzitelli, Silvia; Giustetto, Pierangela; Boffa, Cinzia; Delli Castelli, Daniela; Cutrin, Juan Carlos; et al.; In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound; Elsevier Science; Nanomedicine-nanotechnology Biology And Medicine; 10; 5; 3-2014; 901-904
1549-9634
url http://hdl.handle.net/11336/7867
identifier_str_mv Rizzitelli, Silvia; Giustetto, Pierangela; Boffa, Cinzia; Delli Castelli, Daniela; Cutrin, Juan Carlos; et al.; In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound; Elsevier Science; Nanomedicine-nanotechnology Biology And Medicine; 10; 5; 3-2014; 901-904
1549-9634
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1549963414001300
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nano.2014.03.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614210144698368
score 13.070432

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