Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats

Autores
Brocca, María Elvira; Pietranera, Luciana; Roig, Paulina; Lima, Analia Ethel; de Nicola, Alejandro Federico
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous work has shown a reduction of apical dendritic length and spine density in neurons from the CA1 hippocampus subfield of spontaneously hypertensive rats (SHR). These abnormalities are prevented by treatment for 2 weeks with 17 beta-estradiol. In view of the fact that diabetes and hypertension are comorbid diseases, we have now studied the effect of Streptozotocin-induced diabetes on the dendritic tree and spines of CA1 hippocampus neurons, and also compared the regulation of these parameters by 17 beta-estradiol in diabetic and normoglycemic SHR. Twenty week old male SHR received iv 40 mg/kg Streptozotocin or vehicle and studied one month afterwards. A group of normoglycemic and hyperglycemic SHR also received sc a single 17 beta-estradiol pellet or vehicle for 2 weeks. Hippocampus sections were impregnated with silver nitrate following a modified Golgi´s method and the arbor of CA1 pyramidal neurons analyzed by the Sholl´s method. 17 betaestradiol treatment of normoglycemic SHR reversed the reduced length of apical dendrites, the low spine density and additionally decreased blood pressure. Diabetic SHR showed increased length of apical and basal dendrites but reduced spine density compared to normoglycemic SHR. Diabetes also decreased blood pressure of SHR. Treatment with 17 beta-estradiol of diabetic SHR enhanced dendritic length, increased dendritic spine density and further decreased blood pressure. Thus, changes of cytoarchitecture of CA1 neurons due to 17 beta-estradiol treatment of normoglycemic SHR persisted after diabetes induction. A decrease of blood pressure may also contribute to the central effects of 17beta-estradiol in SHR diabetic rats.
Fil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Materia
Hippocampus
17beta-Estradiol
Dendrites
Spines
Diabetes_Mellitus
Spontaneously_Hypertensive_Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6288
Acceder
id CONICETDig_223a11721e145c6e9ff98d2a862fff0f
oai_identifier_str oai:ri.conicet.gov.ar:11336/6288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive ratsBrocca, María ElviraPietranera, LucianaRoig, PaulinaLima, Analia Ethelde Nicola, Alejandro FedericoHippocampus17beta-EstradiolDendritesSpinesDiabetes_MellitusSpontaneously_Hypertensive_Rathttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous work has shown a reduction of apical dendritic length and spine density in neurons from the CA1 hippocampus subfield of spontaneously hypertensive rats (SHR). These abnormalities are prevented by treatment for 2 weeks with 17 beta-estradiol. In view of the fact that diabetes and hypertension are comorbid diseases, we have now studied the effect of Streptozotocin-induced diabetes on the dendritic tree and spines of CA1 hippocampus neurons, and also compared the regulation of these parameters by 17 beta-estradiol in diabetic and normoglycemic SHR. Twenty week old male SHR received iv 40 mg/kg Streptozotocin or vehicle and studied one month afterwards. A group of normoglycemic and hyperglycemic SHR also received sc a single 17 beta-estradiol pellet or vehicle for 2 weeks. Hippocampus sections were impregnated with silver nitrate following a modified Golgi´s method and the arbor of CA1 pyramidal neurons analyzed by the Sholl´s method. 17 betaestradiol treatment of normoglycemic SHR reversed the reduced length of apical dendrites, the low spine density and additionally decreased blood pressure. Diabetic SHR showed increased length of apical and basal dendrites but reduced spine density compared to normoglycemic SHR. Diabetes also decreased blood pressure of SHR. Treatment with 17 beta-estradiol of diabetic SHR enhanced dendritic length, increased dendritic spine density and further decreased blood pressure. Thus, changes of cytoarchitecture of CA1 neurons due to 17 beta-estradiol treatment of normoglycemic SHR persisted after diabetes induction. A decrease of blood pressure may also contribute to the central effects of 17beta-estradiol in SHR diabetic rats.Fil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaElsevier2014-11-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6288Brocca, María Elvira; Pietranera, Luciana; Roig, Paulina; Lima, Analia Ethel; de Nicola, Alejandro Federico; Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats; Elsevier; Neuroscience; 280; 7-11-2014; 243-2530306-45221873-7544enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2014.09.030info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452214007799info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:34Zoai:ri.conicet.gov.ar:11336/6288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:35.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
title Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
spellingShingle Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
Brocca, María Elvira
Hippocampus
17beta-Estradiol
Dendrites
Spines
Diabetes_Mellitus
Spontaneously_Hypertensive_Rat
title_short Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
title_full Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
title_fullStr Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
title_full_unstemmed Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
title_sort Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats
dc.creator.none.fl_str_mv Brocca, María Elvira
Pietranera, Luciana
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author Brocca, María Elvira
author_facet Brocca, María Elvira
Pietranera, Luciana
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author_role author
author2 Pietranera, Luciana
Roig, Paulina
Lima, Analia Ethel
de Nicola, Alejandro Federico
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hippocampus
17beta-Estradiol
Dendrites
Spines
Diabetes_Mellitus
Spontaneously_Hypertensive_Rat
topic Hippocampus
17beta-Estradiol
Dendrites
Spines
Diabetes_Mellitus
Spontaneously_Hypertensive_Rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous work has shown a reduction of apical dendritic length and spine density in neurons from the CA1 hippocampus subfield of spontaneously hypertensive rats (SHR). These abnormalities are prevented by treatment for 2 weeks with 17 beta-estradiol. In view of the fact that diabetes and hypertension are comorbid diseases, we have now studied the effect of Streptozotocin-induced diabetes on the dendritic tree and spines of CA1 hippocampus neurons, and also compared the regulation of these parameters by 17 beta-estradiol in diabetic and normoglycemic SHR. Twenty week old male SHR received iv 40 mg/kg Streptozotocin or vehicle and studied one month afterwards. A group of normoglycemic and hyperglycemic SHR also received sc a single 17 beta-estradiol pellet or vehicle for 2 weeks. Hippocampus sections were impregnated with silver nitrate following a modified Golgi´s method and the arbor of CA1 pyramidal neurons analyzed by the Sholl´s method. 17 betaestradiol treatment of normoglycemic SHR reversed the reduced length of apical dendrites, the low spine density and additionally decreased blood pressure. Diabetic SHR showed increased length of apical and basal dendrites but reduced spine density compared to normoglycemic SHR. Diabetes also decreased blood pressure of SHR. Treatment with 17 beta-estradiol of diabetic SHR enhanced dendritic length, increased dendritic spine density and further decreased blood pressure. Thus, changes of cytoarchitecture of CA1 neurons due to 17 beta-estradiol treatment of normoglycemic SHR persisted after diabetes induction. A decrease of blood pressure may also contribute to the central effects of 17beta-estradiol in SHR diabetic rats.
Fil: Brocca, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pietranera, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
description Previous work has shown a reduction of apical dendritic length and spine density in neurons from the CA1 hippocampus subfield of spontaneously hypertensive rats (SHR). These abnormalities are prevented by treatment for 2 weeks with 17 beta-estradiol. In view of the fact that diabetes and hypertension are comorbid diseases, we have now studied the effect of Streptozotocin-induced diabetes on the dendritic tree and spines of CA1 hippocampus neurons, and also compared the regulation of these parameters by 17 beta-estradiol in diabetic and normoglycemic SHR. Twenty week old male SHR received iv 40 mg/kg Streptozotocin or vehicle and studied one month afterwards. A group of normoglycemic and hyperglycemic SHR also received sc a single 17 beta-estradiol pellet or vehicle for 2 weeks. Hippocampus sections were impregnated with silver nitrate following a modified Golgi´s method and the arbor of CA1 pyramidal neurons analyzed by the Sholl´s method. 17 betaestradiol treatment of normoglycemic SHR reversed the reduced length of apical dendrites, the low spine density and additionally decreased blood pressure. Diabetic SHR showed increased length of apical and basal dendrites but reduced spine density compared to normoglycemic SHR. Diabetes also decreased blood pressure of SHR. Treatment with 17 beta-estradiol of diabetic SHR enhanced dendritic length, increased dendritic spine density and further decreased blood pressure. Thus, changes of cytoarchitecture of CA1 neurons due to 17 beta-estradiol treatment of normoglycemic SHR persisted after diabetes induction. A decrease of blood pressure may also contribute to the central effects of 17beta-estradiol in SHR diabetic rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6288
Brocca, María Elvira; Pietranera, Luciana; Roig, Paulina; Lima, Analia Ethel; de Nicola, Alejandro Federico; Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats; Elsevier; Neuroscience; 280; 7-11-2014; 243-253
0306-4522
1873-7544
url http://hdl.handle.net/11336/6288
identifier_str_mv Brocca, María Elvira; Pietranera, Luciana; Roig, Paulina; Lima, Analia Ethel; de Nicola, Alejandro Federico; Effects of 17β-estradiol on the cytoarchitecture of pyramidal CA1 neurons in normoglycemic and diabetic male spontaneously hypertensive rats; Elsevier; Neuroscience; 280; 7-11-2014; 243-253
0306-4522
1873-7544
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2014.09.030
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452214007799
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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