Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization

Autores
Blanco, Sebastian; Giacomi, Virginia Soledad; Slobodianiuk, Luciano Gabriel; Frutos, Maria Celia; Carrizo, Luis Horacio; Fanin, Gabriela Elvira; Culasso, Jorge Mario; Gallego, Sandra Veronica
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the gene can be enough for clinical application in an admixed population. This robust technique of simple implementation allows to determine fetal RHD in maternal blood with high sensitivity, specificity, and accuracy. The introduction of fetal RhD genotyping as part of an antenatal screening program constitutes a reliable manner to optimize anti-D prophylaxis; however, it has not been implemented so far in most American countries.
Fil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; Argentina
Fil: Giacomi, Virginia Soledad. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Slobodianiuk, Luciano Gabriel. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Frutos, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Carrizo, Luis Horacio. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Fanin, Gabriela Elvira. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Culasso, Jorge Mario. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Gallego, Sandra Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; Argentina
Materia
ANTENATAL IMMUNOPROPHYLAXIS
FETAL RHD GENOTYPING
HEMOLYTIC DISEASE OF THE NEWBORN
MOLECULAR BLOOD GROUP TYPING
NON-INVASIVE PRENATAL DIAGNOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164419
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/164419
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis OptimizationBlanco, SebastianGiacomi, Virginia SoledadSlobodianiuk, Luciano GabrielFrutos, Maria CeliaCarrizo, Luis HoracioFanin, Gabriela ElviraCulasso, Jorge MarioGallego, Sandra VeronicaANTENATAL IMMUNOPROPHYLAXISFETAL RHD GENOTYPINGHEMOLYTIC DISEASE OF THE NEWBORNMOLECULAR BLOOD GROUP TYPINGNON-INVASIVE PRENATAL DIAGNOSIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the gene can be enough for clinical application in an admixed population. This robust technique of simple implementation allows to determine fetal RHD in maternal blood with high sensitivity, specificity, and accuracy. The introduction of fetal RhD genotyping as part of an antenatal screening program constitutes a reliable manner to optimize anti-D prophylaxis; however, it has not been implemented so far in most American countries.Fil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; ArgentinaFil: Giacomi, Virginia Soledad. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; ArgentinaFil: Slobodianiuk, Luciano Gabriel. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; ArgentinaFil: Frutos, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Carrizo, Luis Horacio. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; ArgentinaFil: Fanin, Gabriela Elvira. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; ArgentinaFil: Culasso, Jorge Mario. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; ArgentinaFil: Gallego, Sandra Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; ArgentinaKarger2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164419Blanco, Sebastian; Giacomi, Virginia Soledad; Slobodianiuk, Luciano Gabriel; Frutos, Maria Celia; Carrizo, Luis Horacio; et al.; Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization; Karger; Transfusion Medicine And Hemotherapy; 45; 6; 11-2018; 423-4281660-37961660-3818CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/490156info:eu-repo/semantics/altIdentifier/doi/10.1159/000490156info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:31Zoai:ri.conicet.gov.ar:11336/164419instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:31.778CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
title Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
spellingShingle Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
Blanco, Sebastian
ANTENATAL IMMUNOPROPHYLAXIS
FETAL RHD GENOTYPING
HEMOLYTIC DISEASE OF THE NEWBORN
MOLECULAR BLOOD GROUP TYPING
NON-INVASIVE PRENATAL DIAGNOSIS
title_short Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
title_full Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
title_fullStr Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
title_full_unstemmed Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
title_sort Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization
dc.creator.none.fl_str_mv Blanco, Sebastian
Giacomi, Virginia Soledad
Slobodianiuk, Luciano Gabriel
Frutos, Maria Celia
Carrizo, Luis Horacio
Fanin, Gabriela Elvira
Culasso, Jorge Mario
Gallego, Sandra Veronica
author Blanco, Sebastian
author_facet Blanco, Sebastian
Giacomi, Virginia Soledad
Slobodianiuk, Luciano Gabriel
Frutos, Maria Celia
Carrizo, Luis Horacio
Fanin, Gabriela Elvira
Culasso, Jorge Mario
Gallego, Sandra Veronica
author_role author
author2 Giacomi, Virginia Soledad
Slobodianiuk, Luciano Gabriel
Frutos, Maria Celia
Carrizo, Luis Horacio
Fanin, Gabriela Elvira
Culasso, Jorge Mario
Gallego, Sandra Veronica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTENATAL IMMUNOPROPHYLAXIS
FETAL RHD GENOTYPING
HEMOLYTIC DISEASE OF THE NEWBORN
MOLECULAR BLOOD GROUP TYPING
NON-INVASIVE PRENATAL DIAGNOSIS
topic ANTENATAL IMMUNOPROPHYLAXIS
FETAL RHD GENOTYPING
HEMOLYTIC DISEASE OF THE NEWBORN
MOLECULAR BLOOD GROUP TYPING
NON-INVASIVE PRENATAL DIAGNOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the gene can be enough for clinical application in an admixed population. This robust technique of simple implementation allows to determine fetal RHD in maternal blood with high sensitivity, specificity, and accuracy. The introduction of fetal RhD genotyping as part of an antenatal screening program constitutes a reliable manner to optimize anti-D prophylaxis; however, it has not been implemented so far in most American countries.
Fil: Blanco, Sebastian. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; Argentina
Fil: Giacomi, Virginia Soledad. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Slobodianiuk, Luciano Gabriel. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Frutos, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Carrizo, Luis Horacio. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Fanin, Gabriela Elvira. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Culasso, Jorge Mario. Fundación Banco Central de Sangre; Argentina. Hospital Materno-Provincial Dr. Raúl Felipe Lucini; Argentina
Fil: Gallego, Sandra Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Fundación Banco Central de Sangre; Argentina
description Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the gene can be enough for clinical application in an admixed population. This robust technique of simple implementation allows to determine fetal RHD in maternal blood with high sensitivity, specificity, and accuracy. The introduction of fetal RhD genotyping as part of an antenatal screening program constitutes a reliable manner to optimize anti-D prophylaxis; however, it has not been implemented so far in most American countries.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164419
Blanco, Sebastian; Giacomi, Virginia Soledad; Slobodianiuk, Luciano Gabriel; Frutos, Maria Celia; Carrizo, Luis Horacio; et al.; Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization; Karger; Transfusion Medicine And Hemotherapy; 45; 6; 11-2018; 423-428
1660-3796
1660-3818
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164419
identifier_str_mv Blanco, Sebastian; Giacomi, Virginia Soledad; Slobodianiuk, Luciano Gabriel; Frutos, Maria Celia; Carrizo, Luis Horacio; et al.; Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization; Karger; Transfusion Medicine And Hemotherapy; 45; 6; 11-2018; 423-428
1660-3796
1660-3818
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/490156
info:eu-repo/semantics/altIdentifier/doi/10.1159/000490156
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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