Eugenol synthase genes in floral scent variation in Gymnadenia species

Autores
Gupta, Alok K.; Abatedaga, Maria Ines de Los Angeles; Pichersky, Eran; Schiestl, Florian P.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Floral signaling, especially through floral scent, is often highly complex, and little is known about the molecular mechanisms and evolutionary causes of this complexity. In this study, we focused on the evolution of “floral scent genes” and the associated changes in their functions in three closely related orchid species of the genus Gymnadenia. We developed a benchmark repertoire of 2,571 expressed sequence tags (ESTs) in Gymnadenia odoratissima. For the functional characterization and evolutionary analysis, we focused on eugenol synthase, as eugenol is a widespread and important scent compound. We obtained complete coding complementary DNAs (cDNAs) of two copies of putative eugenol synthase genes in each of the three species. The proteins encoded by these cDNAs were characterized by expression and testing for activity in Escherichia coli. While G. odoratissima and Gymnadenia conopsea enzymes were found to catalyze the formation of eugenol only, the Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol. Finally, we showed that the eugenol and isoeugenol producing gene copies of G. densiflora are evolutionarily derived from the ancestral genes of the other species producing only eugenol. The evolutionary switch from production of one to two compounds evolved under relaxed purifying selection. In conclusion, our study shows the molecular bases of eugenol and isoeugenol production and suggests that an evolutionary transition in a single gene can lead to an increased complexity in floral scent emitted by plants.
Fil: Gupta, Alok K.. Universitat Zurich; Suiza. Plant Ecological Genetics. Zurich; Suiza
Fil: Abatedaga, Maria Ines de Los Angeles. University of Michigan; Estados Unidos
Fil: Pichersky, Eran. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Schiestl, Florian P.. Universitat Zurich; Suiza
Materia
Ests
Eugenol
Floral Scents
Gymnadenia
Pollination
Isoeugenol
Voc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35887
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Eugenol synthase genes in floral scent variation in Gymnadenia speciesGupta, Alok K.Abatedaga, Maria Ines de Los AngelesPichersky, EranSchiestl, Florian P.EstsEugenolFloral ScentsGymnadeniaPollinationIsoeugenolVochttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Floral signaling, especially through floral scent, is often highly complex, and little is known about the molecular mechanisms and evolutionary causes of this complexity. In this study, we focused on the evolution of “floral scent genes” and the associated changes in their functions in three closely related orchid species of the genus Gymnadenia. We developed a benchmark repertoire of 2,571 expressed sequence tags (ESTs) in Gymnadenia odoratissima. For the functional characterization and evolutionary analysis, we focused on eugenol synthase, as eugenol is a widespread and important scent compound. We obtained complete coding complementary DNAs (cDNAs) of two copies of putative eugenol synthase genes in each of the three species. The proteins encoded by these cDNAs were characterized by expression and testing for activity in Escherichia coli. While G. odoratissima and Gymnadenia conopsea enzymes were found to catalyze the formation of eugenol only, the Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol. Finally, we showed that the eugenol and isoeugenol producing gene copies of G. densiflora are evolutionarily derived from the ancestral genes of the other species producing only eugenol. The evolutionary switch from production of one to two compounds evolved under relaxed purifying selection. In conclusion, our study shows the molecular bases of eugenol and isoeugenol production and suggests that an evolutionary transition in a single gene can lead to an increased complexity in floral scent emitted by plants.Fil: Gupta, Alok K.. Universitat Zurich; Suiza. Plant Ecological Genetics. Zurich; SuizaFil: Abatedaga, Maria Ines de Los Angeles. University of Michigan; Estados UnidosFil: Pichersky, Eran. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Schiestl, Florian P.. Universitat Zurich; SuizaSpringer Heidelberg2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35887Gupta, Alok K.; Abatedaga, Maria Ines de Los Angeles; Pichersky, Eran; Schiestl, Florian P.; Eugenol synthase genes in floral scent variation in Gymnadenia species; Springer Heidelberg; Functional & Integrative Genomics; 14; 4; 9-2014; 779-7881438-793XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10142-014-0397-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10142-014-0397-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:36:53Zoai:ri.conicet.gov.ar:11336/35887instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:36:53.86CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Eugenol synthase genes in floral scent variation in Gymnadenia species
title Eugenol synthase genes in floral scent variation in Gymnadenia species
spellingShingle Eugenol synthase genes in floral scent variation in Gymnadenia species
Gupta, Alok K.
Ests
Eugenol
Floral Scents
Gymnadenia
Pollination
Isoeugenol
Voc
title_short Eugenol synthase genes in floral scent variation in Gymnadenia species
title_full Eugenol synthase genes in floral scent variation in Gymnadenia species
title_fullStr Eugenol synthase genes in floral scent variation in Gymnadenia species
title_full_unstemmed Eugenol synthase genes in floral scent variation in Gymnadenia species
title_sort Eugenol synthase genes in floral scent variation in Gymnadenia species
dc.creator.none.fl_str_mv Gupta, Alok K.
Abatedaga, Maria Ines de Los Angeles
Pichersky, Eran
Schiestl, Florian P.
author Gupta, Alok K.
author_facet Gupta, Alok K.
Abatedaga, Maria Ines de Los Angeles
Pichersky, Eran
Schiestl, Florian P.
author_role author
author2 Abatedaga, Maria Ines de Los Angeles
Pichersky, Eran
Schiestl, Florian P.
author2_role author
author
author
dc.subject.none.fl_str_mv Ests
Eugenol
Floral Scents
Gymnadenia
Pollination
Isoeugenol
Voc
topic Ests
Eugenol
Floral Scents
Gymnadenia
Pollination
Isoeugenol
Voc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Floral signaling, especially through floral scent, is often highly complex, and little is known about the molecular mechanisms and evolutionary causes of this complexity. In this study, we focused on the evolution of “floral scent genes” and the associated changes in their functions in three closely related orchid species of the genus Gymnadenia. We developed a benchmark repertoire of 2,571 expressed sequence tags (ESTs) in Gymnadenia odoratissima. For the functional characterization and evolutionary analysis, we focused on eugenol synthase, as eugenol is a widespread and important scent compound. We obtained complete coding complementary DNAs (cDNAs) of two copies of putative eugenol synthase genes in each of the three species. The proteins encoded by these cDNAs were characterized by expression and testing for activity in Escherichia coli. While G. odoratissima and Gymnadenia conopsea enzymes were found to catalyze the formation of eugenol only, the Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol. Finally, we showed that the eugenol and isoeugenol producing gene copies of G. densiflora are evolutionarily derived from the ancestral genes of the other species producing only eugenol. The evolutionary switch from production of one to two compounds evolved under relaxed purifying selection. In conclusion, our study shows the molecular bases of eugenol and isoeugenol production and suggests that an evolutionary transition in a single gene can lead to an increased complexity in floral scent emitted by plants.
Fil: Gupta, Alok K.. Universitat Zurich; Suiza. Plant Ecological Genetics. Zurich; Suiza
Fil: Abatedaga, Maria Ines de Los Angeles. University of Michigan; Estados Unidos
Fil: Pichersky, Eran. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Schiestl, Florian P.. Universitat Zurich; Suiza
description Floral signaling, especially through floral scent, is often highly complex, and little is known about the molecular mechanisms and evolutionary causes of this complexity. In this study, we focused on the evolution of “floral scent genes” and the associated changes in their functions in three closely related orchid species of the genus Gymnadenia. We developed a benchmark repertoire of 2,571 expressed sequence tags (ESTs) in Gymnadenia odoratissima. For the functional characterization and evolutionary analysis, we focused on eugenol synthase, as eugenol is a widespread and important scent compound. We obtained complete coding complementary DNAs (cDNAs) of two copies of putative eugenol synthase genes in each of the three species. The proteins encoded by these cDNAs were characterized by expression and testing for activity in Escherichia coli. While G. odoratissima and Gymnadenia conopsea enzymes were found to catalyze the formation of eugenol only, the Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol. Finally, we showed that the eugenol and isoeugenol producing gene copies of G. densiflora are evolutionarily derived from the ancestral genes of the other species producing only eugenol. The evolutionary switch from production of one to two compounds evolved under relaxed purifying selection. In conclusion, our study shows the molecular bases of eugenol and isoeugenol production and suggests that an evolutionary transition in a single gene can lead to an increased complexity in floral scent emitted by plants.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35887
Gupta, Alok K.; Abatedaga, Maria Ines de Los Angeles; Pichersky, Eran; Schiestl, Florian P.; Eugenol synthase genes in floral scent variation in Gymnadenia species; Springer Heidelberg; Functional & Integrative Genomics; 14; 4; 9-2014; 779-788
1438-793X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35887
identifier_str_mv Gupta, Alok K.; Abatedaga, Maria Ines de Los Angeles; Pichersky, Eran; Schiestl, Florian P.; Eugenol synthase genes in floral scent variation in Gymnadenia species; Springer Heidelberg; Functional & Integrative Genomics; 14; 4; 9-2014; 779-788
1438-793X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10142-014-0397-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10142-014-0397-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.214268

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