Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti

Autores
Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; Rubio, Ramón G.; Coviella, Carlos Eduardo; Guzmán, Eduardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.
Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Materia
THYMOL
EUGENOL
NANOEMULSIONS
AEDES AEGYPTI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174500
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/174500
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegyptiLucia, AlejandroToloza, Ariel CeferinoFanucce, MicaelaFernandez Peña, LauraOrtega, FranciscoRubio, Ramón G.Coviella, Carlos EduardoGuzmán, EduardoTHYMOLEUGENOLNANOEMULSIONSAEDES AEGYPTIhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaAlma Mater Studiorum, Univ Bologna2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174500Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-81721-8861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bulletinofinsectology.org/pdfarticles/vol73-2020-153-160lucia.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:02Zoai:ri.conicet.gov.ar:11336/174500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:02.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
title Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
spellingShingle Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
Lucia, Alejandro
THYMOL
EUGENOL
NANOEMULSIONS
AEDES AEGYPTI
title_short Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
title_full Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
title_fullStr Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
title_full_unstemmed Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
title_sort Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
dc.creator.none.fl_str_mv Lucia, Alejandro
Toloza, Ariel Ceferino
Fanucce, Micaela
Fernandez Peña, Laura
Ortega, Francisco
Rubio, Ramón G.
Coviella, Carlos Eduardo
Guzmán, Eduardo
author Lucia, Alejandro
author_facet Lucia, Alejandro
Toloza, Ariel Ceferino
Fanucce, Micaela
Fernandez Peña, Laura
Ortega, Francisco
Rubio, Ramón G.
Coviella, Carlos Eduardo
Guzmán, Eduardo
author_role author
author2 Toloza, Ariel Ceferino
Fanucce, Micaela
Fernandez Peña, Laura
Ortega, Francisco
Rubio, Ramón G.
Coviella, Carlos Eduardo
Guzmán, Eduardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv THYMOL
EUGENOL
NANOEMULSIONS
AEDES AEGYPTI
topic THYMOL
EUGENOL
NANOEMULSIONS
AEDES AEGYPTI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.
Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
description Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174500
Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-8
1721-8861
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174500
identifier_str_mv Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-8
1721-8861
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bulletinofinsectology.org/pdfarticles/vol73-2020-153-160lucia.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Alma Mater Studiorum, Univ Bologna
publisher.none.fl_str_mv Alma Mater Studiorum, Univ Bologna
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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