Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti
- Autores
- Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; Rubio, Ramón G.; Coviella, Carlos Eduardo; Guzmán, Eduardo
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.
Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España - Materia
-
THYMOL
EUGENOL
NANOEMULSIONS
AEDES AEGYPTI - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/174500
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Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegyptiLucia, AlejandroToloza, Ariel CeferinoFanucce, MicaelaFernandez Peña, LauraOrtega, FranciscoRubio, Ramón G.Coviella, Carlos EduardoGuzmán, EduardoTHYMOLEUGENOLNANOEMULSIONSAEDES AEGYPTIhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos.Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaAlma Mater Studiorum, Univ Bologna2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174500Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-81721-8861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bulletinofinsectology.org/pdfarticles/vol73-2020-153-160lucia.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:34Zoai:ri.conicet.gov.ar:11336/174500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:35.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
title |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
spellingShingle |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti Lucia, Alejandro THYMOL EUGENOL NANOEMULSIONS AEDES AEGYPTI |
title_short |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
title_full |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
title_fullStr |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
title_full_unstemmed |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
title_sort |
Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti |
dc.creator.none.fl_str_mv |
Lucia, Alejandro Toloza, Ariel Ceferino Fanucce, Micaela Fernandez Peña, Laura Ortega, Francisco Rubio, Ramón G. Coviella, Carlos Eduardo Guzmán, Eduardo |
author |
Lucia, Alejandro |
author_facet |
Lucia, Alejandro Toloza, Ariel Ceferino Fanucce, Micaela Fernandez Peña, Laura Ortega, Francisco Rubio, Ramón G. Coviella, Carlos Eduardo Guzmán, Eduardo |
author_role |
author |
author2 |
Toloza, Ariel Ceferino Fanucce, Micaela Fernandez Peña, Laura Ortega, Francisco Rubio, Ramón G. Coviella, Carlos Eduardo Guzmán, Eduardo |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
THYMOL EUGENOL NANOEMULSIONS AEDES AEGYPTI |
topic |
THYMOL EUGENOL NANOEMULSIONS AEDES AEGYPTI |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos. Fil: Lucia, Alejandro. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina Fil: Fanucce, Micaela. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina Fil: Fernandez Peña, Laura. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España |
description |
Dengue, Zika, chikungunya and yellow fever are the most important vector-borne diseases worldwide transmitted to humans by Aedes aegypti (L.) (Diptera Culicidae). Thus, the control of this vector is of vital importance in order to avoid epidemics in tropical and neo-tropical areas. To find new and effective larvicidal formulations for control programs against mosquito populations, aqueous dispersions containing thymol and eugenol were characterized and tested against Ae. aegypti larvae. The dispersion and stabilization of thymol and eugenol in water was possible using a triblock copolymer with two lateral blocks of poly(ethylene oxide) and a central block of poly(propylene oxide), the so-called poloxamer 407, which allows obtaining oil in water (o/w) emulsion. Dynamic Light Scattering (DLS) points out that emulsions containing eugenol were in most cases monodisperse with an average apparent hydrodynamic diameter of the droplets in the 20-25 nm without destabilization after 28 months from their preparation, which plays a key role for the potential application of the studied formulations. On the other side, those emulsions containing only thymol as oilphase had higher polydispersity, suggesting a central role of eugenol in the dispersion of thymol in water. Furthermore, the combined effect of thymol and eugenol against Ae. aegypti larvae was evaluated. The nanoemulsion containing thymol as main component of the oil phase (100%) showed the lowest LC50 and the introduction of eugenol to the nanoemulsions facilitated the dispersion andstability of thymol in water, even though reducing the effectiveness of the emulsions. The findings on the larvicidal effects of the combined application of the monoterpenes tested could be considered a promising contribution to the development of botanicalderived larvicidal formulations against mosquitos. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/174500 Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-8 1721-8861 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/174500 |
identifier_str_mv |
Lucia, Alejandro; Toloza, Ariel Ceferino; Fanucce, Micaela; Fernandez Peña, Laura; Ortega, Francisco; et al.; Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti; Alma Mater Studiorum, Univ Bologna; Bulletin Of Insectology; 4-2020; 1-8 1721-8861 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.bulletinofinsectology.org/pdfarticles/vol73-2020-153-160lucia.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Alma Mater Studiorum, Univ Bologna |
publisher.none.fl_str_mv |
Alma Mater Studiorum, Univ Bologna |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614362898104320 |
score |
13.070432 |