The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea

Autores
Cantu, D.; Vicente, Ariel Roberto; Greve, L. C.; Dewey, F. M.; Bennett, A. B.; Labavitch, J. M.; Powell, A. L. T.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution. © 2008 by The National Academy of Sciences of the USA.
Fil: Cantu, D.. University of California at Davis; Estados Unidos
Fil: Vicente, Ariel Roberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Greve, L. C.. University of California at Davis; Estados Unidos
Fil: Dewey, F. M.. University of California at Davis; Estados Unidos. University of Oxford; Reino Unido
Fil: Bennett, A. B.. University of California at Davis; Estados Unidos
Fil: Labavitch, J. M.. University of California at Davis; Estados Unidos
Fil: Powell, A. L. T.. University of California at Davis; Estados Unidos
Materia
EXPANSIN
PLANT PATHOGEN
POLYGALACTURONASE
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148748
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinereaCantu, D.Vicente, Ariel RobertoGreve, L. C.Dewey, F. M.Bennett, A. B.Labavitch, J. M.Powell, A. L. T.EXPANSINPLANT PATHOGENPOLYGALACTURONASETOMATOhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution. © 2008 by The National Academy of Sciences of the USA.Fil: Cantu, D.. University of California at Davis; Estados UnidosFil: Vicente, Ariel Roberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Greve, L. C.. University of California at Davis; Estados UnidosFil: Dewey, F. M.. University of California at Davis; Estados Unidos. University of Oxford; Reino UnidoFil: Bennett, A. B.. University of California at Davis; Estados UnidosFil: Labavitch, J. M.. University of California at Davis; Estados UnidosFil: Powell, A. L. T.. University of California at Davis; Estados UnidosNational Academy of Sciences2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148748Cantu, D.; Vicente, Ariel Roberto; Greve, L. C.; Dewey, F. M.; Bennett, A. B.; et al.; The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 105; 3; 1-2008; 859-8640027-84241091-6490CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0709813105info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/105/3/859info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:55:28Zoai:ri.conicet.gov.ar:11336/148748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:55:28.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
title The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
spellingShingle The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
Cantu, D.
EXPANSIN
PLANT PATHOGEN
POLYGALACTURONASE
TOMATO
title_short The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
title_full The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
title_fullStr The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
title_full_unstemmed The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
title_sort The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
dc.creator.none.fl_str_mv Cantu, D.
Vicente, Ariel Roberto
Greve, L. C.
Dewey, F. M.
Bennett, A. B.
Labavitch, J. M.
Powell, A. L. T.
author Cantu, D.
author_facet Cantu, D.
Vicente, Ariel Roberto
Greve, L. C.
Dewey, F. M.
Bennett, A. B.
Labavitch, J. M.
Powell, A. L. T.
author_role author
author2 Vicente, Ariel Roberto
Greve, L. C.
Dewey, F. M.
Bennett, A. B.
Labavitch, J. M.
Powell, A. L. T.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EXPANSIN
PLANT PATHOGEN
POLYGALACTURONASE
TOMATO
topic EXPANSIN
PLANT PATHOGEN
POLYGALACTURONASE
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution. © 2008 by The National Academy of Sciences of the USA.
Fil: Cantu, D.. University of California at Davis; Estados Unidos
Fil: Vicente, Ariel Roberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Greve, L. C.. University of California at Davis; Estados Unidos
Fil: Dewey, F. M.. University of California at Davis; Estados Unidos. University of Oxford; Reino Unido
Fil: Bennett, A. B.. University of California at Davis; Estados Unidos
Fil: Labavitch, J. M.. University of California at Davis; Estados Unidos
Fil: Powell, A. L. T.. University of California at Davis; Estados Unidos
description Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution. © 2008 by The National Academy of Sciences of the USA.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148748
Cantu, D.; Vicente, Ariel Roberto; Greve, L. C.; Dewey, F. M.; Bennett, A. B.; et al.; The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 105; 3; 1-2008; 859-864
0027-8424
1091-6490
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148748
identifier_str_mv Cantu, D.; Vicente, Ariel Roberto; Greve, L. C.; Dewey, F. M.; Bennett, A. B.; et al.; The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 105; 3; 1-2008; 859-864
0027-8424
1091-6490
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0709813105
info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/105/3/859
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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