The TTC-technique might not appropriately test the physiological stage of plant tissues

Autores
Busso, Carlos Alberto; Torres, Yanina Alejandra; Ithurrart, Leticia Soledad; Richards, J. H.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The 2,3,5triphenyltetrazolium chloride (TTC) technique has been used during decades to distin guish between dead and alive tissues of perennial grasses. This technique did not consider, however, that dormant (i.e., viable) tissues could exist within those erroneously considered dead tissues, thus being unable to report the true physiological stage of those plant tissues. Development of a procedure able to distinguish between metabol ically active or dormant or dead tissues is then critical. This study developed a procedure to classify plant tissues of perennial grasses (Poa ligularis (Nees ex Steud.), Nassella longiglumis (Phil.) Barkworth, Amelichloa ambigua (Speg.) Arriaga & Barkworth, and Piptochaetium napostaense (Speg.) Hack.) in a more appropriate physiologi cal stage (i.e., metabolically active, dormant or dead) than the traditional TTCtechnique. Perennial grass seeds or meristematic buds were immersed in a TTC solution to obtain metabolically active (red or pink staining) or unstained (either dormant or dead) tissues. TTCunstained tissues were placed in Evans Blue solution to sepa rate dormant from dead tissues. The combination of TTC and Evans Blue techniques allowed the separation of metabolically active, dormant or dead tissues. Use of Evans Blue on TTCunstained seeds and buds allowed to determine that some of these tissues were not dead but dormant (i.e., viable). Among the TTCunstained tissues between 2 to 35% of total grass seeds and from 19.5 to 42% of all evaluated buds were dormant (viable and poten tially able to growout) but not dead. Combination of TTC and Evans Blue techniques allowed a better classifi cation of the physiological stages of plant tissues (metabolically active, dormant or dead) than the conventional TTC test.
Fil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Richards, J. H.. University of California; Estados Unidos
Materia
Poa Ligularis
Nassella Longiglumis
Amelichloa Ambigua
Piptochaetium Napostaense
Seeds
Axillary Buds
Dead an Alive Tissues
Ttc-Technique
Evans Blue Technique
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11421
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11421
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The TTC-technique might not appropriately test the physiological stage of plant tissuesBusso, Carlos AlbertoTorres, Yanina AlejandraIthurrart, Leticia SoledadRichards, J. H.Poa LigularisNassella LongiglumisAmelichloa AmbiguaPiptochaetium NapostaenseSeedsAxillary BudsDead an Alive TissuesTtc-TechniqueEvans Blue Techniquehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The 2,3,5triphenyltetrazolium chloride (TTC) technique has been used during decades to distin guish between dead and alive tissues of perennial grasses. This technique did not consider, however, that dormant (i.e., viable) tissues could exist within those erroneously considered dead tissues, thus being unable to report the true physiological stage of those plant tissues. Development of a procedure able to distinguish between metabol ically active or dormant or dead tissues is then critical. This study developed a procedure to classify plant tissues of perennial grasses (Poa ligularis (Nees ex Steud.), Nassella longiglumis (Phil.) Barkworth, Amelichloa ambigua (Speg.) Arriaga & Barkworth, and Piptochaetium napostaense (Speg.) Hack.) in a more appropriate physiologi cal stage (i.e., metabolically active, dormant or dead) than the traditional TTCtechnique. Perennial grass seeds or meristematic buds were immersed in a TTC solution to obtain metabolically active (red or pink staining) or unstained (either dormant or dead) tissues. TTCunstained tissues were placed in Evans Blue solution to sepa rate dormant from dead tissues. The combination of TTC and Evans Blue techniques allowed the separation of metabolically active, dormant or dead tissues. Use of Evans Blue on TTCunstained seeds and buds allowed to determine that some of these tissues were not dead but dormant (i.e., viable). Among the TTCunstained tissues between 2 to 35% of total grass seeds and from 19.5 to 42% of all evaluated buds were dormant (viable and poten tially able to growout) but not dead. Combination of TTC and Evans Blue techniques allowed a better classifi cation of the physiological stages of plant tissues (metabolically active, dormant or dead) than the conventional TTC test.Fil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Richards, J. H.. University of California; Estados UnidosSpringer2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11421Busso, Carlos Alberto; Torres, Yanina Alejandra; Ithurrart, Leticia Soledad; Richards, J. H.; The TTC-technique might not appropriately test the physiological stage of plant tissues; Springer; Russian Journal Of Plant Physiology; 62; 4; 7-2015; 551-5561021-44371608-3407enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134%2FS1021443715040068info:eu-repo/semantics/altIdentifier/doi/10.1134/S1021443715040068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:26Zoai:ri.conicet.gov.ar:11336/11421instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:26.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The TTC-technique might not appropriately test the physiological stage of plant tissues
title The TTC-technique might not appropriately test the physiological stage of plant tissues
spellingShingle The TTC-technique might not appropriately test the physiological stage of plant tissues
Busso, Carlos Alberto
Poa Ligularis
Nassella Longiglumis
Amelichloa Ambigua
Piptochaetium Napostaense
Seeds
Axillary Buds
Dead an Alive Tissues
Ttc-Technique
Evans Blue Technique
title_short The TTC-technique might not appropriately test the physiological stage of plant tissues
title_full The TTC-technique might not appropriately test the physiological stage of plant tissues
title_fullStr The TTC-technique might not appropriately test the physiological stage of plant tissues
title_full_unstemmed The TTC-technique might not appropriately test the physiological stage of plant tissues
title_sort The TTC-technique might not appropriately test the physiological stage of plant tissues
dc.creator.none.fl_str_mv Busso, Carlos Alberto
Torres, Yanina Alejandra
Ithurrart, Leticia Soledad
Richards, J. H.
author Busso, Carlos Alberto
author_facet Busso, Carlos Alberto
Torres, Yanina Alejandra
Ithurrart, Leticia Soledad
Richards, J. H.
author_role author
author2 Torres, Yanina Alejandra
Ithurrart, Leticia Soledad
Richards, J. H.
author2_role author
author
author
dc.subject.none.fl_str_mv Poa Ligularis
Nassella Longiglumis
Amelichloa Ambigua
Piptochaetium Napostaense
Seeds
Axillary Buds
Dead an Alive Tissues
Ttc-Technique
Evans Blue Technique
topic Poa Ligularis
Nassella Longiglumis
Amelichloa Ambigua
Piptochaetium Napostaense
Seeds
Axillary Buds
Dead an Alive Tissues
Ttc-Technique
Evans Blue Technique
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The 2,3,5triphenyltetrazolium chloride (TTC) technique has been used during decades to distin guish between dead and alive tissues of perennial grasses. This technique did not consider, however, that dormant (i.e., viable) tissues could exist within those erroneously considered dead tissues, thus being unable to report the true physiological stage of those plant tissues. Development of a procedure able to distinguish between metabol ically active or dormant or dead tissues is then critical. This study developed a procedure to classify plant tissues of perennial grasses (Poa ligularis (Nees ex Steud.), Nassella longiglumis (Phil.) Barkworth, Amelichloa ambigua (Speg.) Arriaga & Barkworth, and Piptochaetium napostaense (Speg.) Hack.) in a more appropriate physiologi cal stage (i.e., metabolically active, dormant or dead) than the traditional TTCtechnique. Perennial grass seeds or meristematic buds were immersed in a TTC solution to obtain metabolically active (red or pink staining) or unstained (either dormant or dead) tissues. TTCunstained tissues were placed in Evans Blue solution to sepa rate dormant from dead tissues. The combination of TTC and Evans Blue techniques allowed the separation of metabolically active, dormant or dead tissues. Use of Evans Blue on TTCunstained seeds and buds allowed to determine that some of these tissues were not dead but dormant (i.e., viable). Among the TTCunstained tissues between 2 to 35% of total grass seeds and from 19.5 to 42% of all evaluated buds were dormant (viable and poten tially able to growout) but not dead. Combination of TTC and Evans Blue techniques allowed a better classifi cation of the physiological stages of plant tissues (metabolically active, dormant or dead) than the conventional TTC test.
Fil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Richards, J. H.. University of California; Estados Unidos
description The 2,3,5triphenyltetrazolium chloride (TTC) technique has been used during decades to distin guish between dead and alive tissues of perennial grasses. This technique did not consider, however, that dormant (i.e., viable) tissues could exist within those erroneously considered dead tissues, thus being unable to report the true physiological stage of those plant tissues. Development of a procedure able to distinguish between metabol ically active or dormant or dead tissues is then critical. This study developed a procedure to classify plant tissues of perennial grasses (Poa ligularis (Nees ex Steud.), Nassella longiglumis (Phil.) Barkworth, Amelichloa ambigua (Speg.) Arriaga & Barkworth, and Piptochaetium napostaense (Speg.) Hack.) in a more appropriate physiologi cal stage (i.e., metabolically active, dormant or dead) than the traditional TTCtechnique. Perennial grass seeds or meristematic buds were immersed in a TTC solution to obtain metabolically active (red or pink staining) or unstained (either dormant or dead) tissues. TTCunstained tissues were placed in Evans Blue solution to sepa rate dormant from dead tissues. The combination of TTC and Evans Blue techniques allowed the separation of metabolically active, dormant or dead tissues. Use of Evans Blue on TTCunstained seeds and buds allowed to determine that some of these tissues were not dead but dormant (i.e., viable). Among the TTCunstained tissues between 2 to 35% of total grass seeds and from 19.5 to 42% of all evaluated buds were dormant (viable and poten tially able to growout) but not dead. Combination of TTC and Evans Blue techniques allowed a better classifi cation of the physiological stages of plant tissues (metabolically active, dormant or dead) than the conventional TTC test.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11421
Busso, Carlos Alberto; Torres, Yanina Alejandra; Ithurrart, Leticia Soledad; Richards, J. H.; The TTC-technique might not appropriately test the physiological stage of plant tissues; Springer; Russian Journal Of Plant Physiology; 62; 4; 7-2015; 551-556
1021-4437
1608-3407
url http://hdl.handle.net/11336/11421
identifier_str_mv Busso, Carlos Alberto; Torres, Yanina Alejandra; Ithurrart, Leticia Soledad; Richards, J. H.; The TTC-technique might not appropriately test the physiological stage of plant tissues; Springer; Russian Journal Of Plant Physiology; 62; 4; 7-2015; 551-556
1021-4437
1608-3407
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134%2FS1021443715040068
info:eu-repo/semantics/altIdentifier/doi/10.1134/S1021443715040068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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