A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats

Autores
Hernando Insúa, Andrés; Rodriguez, Juan M.; Elías, Fernanda; Fló, Juan; López, Ricardo; Franco, Raul; Lago, Nestor; Zorzopulos, Jorge; Montaner, Alejandro Daniel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Synthetic oligodeoxynucleotides (ODNs) are currently being evaluated as vaccine adjuvants for inducing protective immunity. As maternal vaccination is becoming increasingly common, the potential risk of vaccine formulation using ODN adjuvants should be warranted. A recent study performed in mice suggests that exposure to CpG motifs during pregnancy could result (although at very high doses as compared to the ones proposed for human vaccination) in fetal loss and morphological defects. PyNTTTTGT ODNs are immunostimulatory ODNs not bearing CpG motifs, which are very efficient vaccine adjuvants. In this report, we analyzed the potential teratogenic effect of its prototype IMT504 in rats. This animal model was chosen because PyNTTTTGT ODNs are barely active in mice. Intraperitoneal injection of IMT504 at a dose of 20 mg/kg (more than 1000 times higher than the one proposed for a vaccine dose in humans) at day 6 of pregnancy did not produce a significant decrease in the mean number of implanted fetuses or in the number of live pups delivered. Neither the fetuses nor the offspring presented malformations.
Fil: Hernando Insúa, Andrés. Immunotech; Argentina
Fil: Rodriguez, Juan M.. Immunotech; Argentina
Fil: Elías, Fernanda. Immunotech; Argentina
Fil: Fló, Juan. Immunotech; Argentina
Fil: López, Ricardo. Immunotech; Argentina
Fil: Franco, Raul. Immunotech; Argentina
Fil: Lago, Nestor. Gema Biotech; Argentina
Fil: Zorzopulos, Jorge. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaner, Alejandro Daniel. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
Materia
Pynttttgt Odn
Oligonucleotides
Toxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68917
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68917
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in ratsHernando Insúa, AndrésRodriguez, Juan M.Elías, FernandaFló, JuanLópez, RicardoFranco, RaulLago, NestorZorzopulos, JorgeMontaner, Alejandro DanielPynttttgt OdnOligonucleotidesToxicityhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Synthetic oligodeoxynucleotides (ODNs) are currently being evaluated as vaccine adjuvants for inducing protective immunity. As maternal vaccination is becoming increasingly common, the potential risk of vaccine formulation using ODN adjuvants should be warranted. A recent study performed in mice suggests that exposure to CpG motifs during pregnancy could result (although at very high doses as compared to the ones proposed for human vaccination) in fetal loss and morphological defects. PyNTTTTGT ODNs are immunostimulatory ODNs not bearing CpG motifs, which are very efficient vaccine adjuvants. In this report, we analyzed the potential teratogenic effect of its prototype IMT504 in rats. This animal model was chosen because PyNTTTTGT ODNs are barely active in mice. Intraperitoneal injection of IMT504 at a dose of 20 mg/kg (more than 1000 times higher than the one proposed for a vaccine dose in humans) at day 6 of pregnancy did not produce a significant decrease in the mean number of implanted fetuses or in the number of live pups delivered. Neither the fetuses nor the offspring presented malformations.Fil: Hernando Insúa, Andrés. Immunotech; ArgentinaFil: Rodriguez, Juan M.. Immunotech; ArgentinaFil: Elías, Fernanda. Immunotech; ArgentinaFil: Fló, Juan. Immunotech; ArgentinaFil: López, Ricardo. Immunotech; ArgentinaFil: Franco, Raul. Immunotech; ArgentinaFil: Lago, Nestor. Gema Biotech; ArgentinaFil: Zorzopulos, Jorge. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montaner, Alejandro Daniel. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; ArgentinaMary Ann Liebert2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68917Hernando Insúa, Andrés; Rodriguez, Juan M.; Elías, Fernanda; Fló, Juan; López, Ricardo; et al.; A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats; Mary Ann Liebert; Oligonucleotides; 20; 1; 2-2010; 33-351545-4576CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/oli.2009.0206info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/oli.2009.0206info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:48Zoai:ri.conicet.gov.ar:11336/68917instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:48.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
title A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
spellingShingle A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
Hernando Insúa, Andrés
Pynttttgt Odn
Oligonucleotides
Toxicity
title_short A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
title_full A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
title_fullStr A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
title_full_unstemmed A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
title_sort A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats
dc.creator.none.fl_str_mv Hernando Insúa, Andrés
Rodriguez, Juan M.
Elías, Fernanda
Fló, Juan
López, Ricardo
Franco, Raul
Lago, Nestor
Zorzopulos, Jorge
Montaner, Alejandro Daniel
author Hernando Insúa, Andrés
author_facet Hernando Insúa, Andrés
Rodriguez, Juan M.
Elías, Fernanda
Fló, Juan
López, Ricardo
Franco, Raul
Lago, Nestor
Zorzopulos, Jorge
Montaner, Alejandro Daniel
author_role author
author2 Rodriguez, Juan M.
Elías, Fernanda
Fló, Juan
López, Ricardo
Franco, Raul
Lago, Nestor
Zorzopulos, Jorge
Montaner, Alejandro Daniel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pynttttgt Odn
Oligonucleotides
Toxicity
topic Pynttttgt Odn
Oligonucleotides
Toxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Synthetic oligodeoxynucleotides (ODNs) are currently being evaluated as vaccine adjuvants for inducing protective immunity. As maternal vaccination is becoming increasingly common, the potential risk of vaccine formulation using ODN adjuvants should be warranted. A recent study performed in mice suggests that exposure to CpG motifs during pregnancy could result (although at very high doses as compared to the ones proposed for human vaccination) in fetal loss and morphological defects. PyNTTTTGT ODNs are immunostimulatory ODNs not bearing CpG motifs, which are very efficient vaccine adjuvants. In this report, we analyzed the potential teratogenic effect of its prototype IMT504 in rats. This animal model was chosen because PyNTTTTGT ODNs are barely active in mice. Intraperitoneal injection of IMT504 at a dose of 20 mg/kg (more than 1000 times higher than the one proposed for a vaccine dose in humans) at day 6 of pregnancy did not produce a significant decrease in the mean number of implanted fetuses or in the number of live pups delivered. Neither the fetuses nor the offspring presented malformations.
Fil: Hernando Insúa, Andrés. Immunotech; Argentina
Fil: Rodriguez, Juan M.. Immunotech; Argentina
Fil: Elías, Fernanda. Immunotech; Argentina
Fil: Fló, Juan. Immunotech; Argentina
Fil: López, Ricardo. Immunotech; Argentina
Fil: Franco, Raul. Immunotech; Argentina
Fil: Lago, Nestor. Gema Biotech; Argentina
Fil: Zorzopulos, Jorge. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaner, Alejandro Daniel. Immunotech; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
description Synthetic oligodeoxynucleotides (ODNs) are currently being evaluated as vaccine adjuvants for inducing protective immunity. As maternal vaccination is becoming increasingly common, the potential risk of vaccine formulation using ODN adjuvants should be warranted. A recent study performed in mice suggests that exposure to CpG motifs during pregnancy could result (although at very high doses as compared to the ones proposed for human vaccination) in fetal loss and morphological defects. PyNTTTTGT ODNs are immunostimulatory ODNs not bearing CpG motifs, which are very efficient vaccine adjuvants. In this report, we analyzed the potential teratogenic effect of its prototype IMT504 in rats. This animal model was chosen because PyNTTTTGT ODNs are barely active in mice. Intraperitoneal injection of IMT504 at a dose of 20 mg/kg (more than 1000 times higher than the one proposed for a vaccine dose in humans) at day 6 of pregnancy did not produce a significant decrease in the mean number of implanted fetuses or in the number of live pups delivered. Neither the fetuses nor the offspring presented malformations.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68917
Hernando Insúa, Andrés; Rodriguez, Juan M.; Elías, Fernanda; Fló, Juan; López, Ricardo; et al.; A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats; Mary Ann Liebert; Oligonucleotides; 20; 1; 2-2010; 33-35
1545-4576
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68917
identifier_str_mv Hernando Insúa, Andrés; Rodriguez, Juan M.; Elías, Fernanda; Fló, Juan; López, Ricardo; et al.; A high dose of IMT504, the PyNTTTTGT prototype immunostimulatory oligonucleotide, does not alter embryonic development in rats; Mary Ann Liebert; Oligonucleotides; 20; 1; 2-2010; 33-35
1545-4576
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/oli.2009.0206
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/oli.2009.0206
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270018329378816
score 13.13397

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