Simulation of 1d and 2d electrophoretic separations in microfluidics chips

Autores
Kler, Pablo Alejandro; Guarnieri, Fabio Ariel; Berli, Claudio Luis Alberto
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free flow electrophoresis (FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an external electric field. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on microfluidic devices was presented. The model is based on the set of equations that governs electrical phenomena (Poisson equation), fluid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength, pH) and surface potential of channel walls is taken into consideration. In this work, three application examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the influence of electro-osmotic flow on its performance, (b) an IEF assay involving ampholyte-based pH gradient, and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by using PETSc-FEM (Portable Extensible Toolkit for Scientific Computation - Finite Elements Method), in a Python environment developed at CIMEC using high performance parallel computing and solving techniques based on domain decomposition methods.
Fil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Guarnieri, Fabio Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional de Entre Rios; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Materia
MICROFLUIDIC CHIPS
ELECTROPHORESIS
NUMERICAL MODEL
PETSC-FEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17121
Acceder
id CONICETDig_207e97c266a7e1d21b8ff92de029c245
oai_identifier_str oai:ri.conicet.gov.ar:11336/17121
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simulation of 1d and 2d electrophoretic separations in microfluidics chipsKler, Pablo AlejandroGuarnieri, Fabio ArielBerli, Claudio Luis AlbertoMICROFLUIDIC CHIPSELECTROPHORESISNUMERICAL MODELPETSC-FEMhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free flow electrophoresis (FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an external electric field. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on microfluidic devices was presented. The model is based on the set of equations that governs electrical phenomena (Poisson equation), fluid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength, pH) and surface potential of channel walls is taken into consideration. In this work, three application examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the influence of electro-osmotic flow on its performance, (b) an IEF assay involving ampholyte-based pH gradient, and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by using PETSc-FEM (Portable Extensible Toolkit for Scientific Computation - Finite Elements Method), in a Python environment developed at CIMEC using high performance parallel computing and solving techniques based on domain decomposition methods.Fil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Guarnieri, Fabio Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional de Entre Rios; ArgentinaFil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaAsociación Argentina de Mecánica Computacional2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17121Kler, Pablo Alejandro; Guarnieri, Fabio Ariel; Berli, Claudio Luis Alberto; Simulation of 1d and 2d electrophoretic separations in microfluidics chips ; Asociación Argentina de Mecánica Computacional; Mecanica Computacional; XXVIII; 24; 11-2009; 2053-20701666-6070enginfo:eu-repo/semantics/altIdentifier/url/http://www.cimec.org.ar/ojs/index.php/mc/article/view/2859info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:44Zoai:ri.conicet.gov.ar:11336/17121instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:44.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simulation of 1d and 2d electrophoretic separations in microfluidics chips
title Simulation of 1d and 2d electrophoretic separations in microfluidics chips
spellingShingle Simulation of 1d and 2d electrophoretic separations in microfluidics chips
Kler, Pablo Alejandro
MICROFLUIDIC CHIPS
ELECTROPHORESIS
NUMERICAL MODEL
PETSC-FEM
title_short Simulation of 1d and 2d electrophoretic separations in microfluidics chips
title_full Simulation of 1d and 2d electrophoretic separations in microfluidics chips
title_fullStr Simulation of 1d and 2d electrophoretic separations in microfluidics chips
title_full_unstemmed Simulation of 1d and 2d electrophoretic separations in microfluidics chips
title_sort Simulation of 1d and 2d electrophoretic separations in microfluidics chips
dc.creator.none.fl_str_mv Kler, Pablo Alejandro
Guarnieri, Fabio Ariel
Berli, Claudio Luis Alberto
author Kler, Pablo Alejandro
author_facet Kler, Pablo Alejandro
Guarnieri, Fabio Ariel
Berli, Claudio Luis Alberto
author_role author
author2 Guarnieri, Fabio Ariel
Berli, Claudio Luis Alberto
author2_role author
author
dc.subject.none.fl_str_mv MICROFLUIDIC CHIPS
ELECTROPHORESIS
NUMERICAL MODEL
PETSC-FEM
topic MICROFLUIDIC CHIPS
ELECTROPHORESIS
NUMERICAL MODEL
PETSC-FEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free flow electrophoresis (FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an external electric field. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on microfluidic devices was presented. The model is based on the set of equations that governs electrical phenomena (Poisson equation), fluid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength, pH) and surface potential of channel walls is taken into consideration. In this work, three application examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the influence of electro-osmotic flow on its performance, (b) an IEF assay involving ampholyte-based pH gradient, and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by using PETSc-FEM (Portable Extensible Toolkit for Scientific Computation - Finite Elements Method), in a Python environment developed at CIMEC using high performance parallel computing and solving techniques based on domain decomposition methods.
Fil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Guarnieri, Fabio Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional de Entre Rios; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
description Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free flow electrophoresis (FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an external electric field. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on microfluidic devices was presented. The model is based on the set of equations that governs electrical phenomena (Poisson equation), fluid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength, pH) and surface potential of channel walls is taken into consideration. In this work, three application examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the influence of electro-osmotic flow on its performance, (b) an IEF assay involving ampholyte-based pH gradient, and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by using PETSc-FEM (Portable Extensible Toolkit for Scientific Computation - Finite Elements Method), in a Python environment developed at CIMEC using high performance parallel computing and solving techniques based on domain decomposition methods.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17121
Kler, Pablo Alejandro; Guarnieri, Fabio Ariel; Berli, Claudio Luis Alberto; Simulation of 1d and 2d electrophoretic separations in microfluidics chips ; Asociación Argentina de Mecánica Computacional; Mecanica Computacional; XXVIII; 24; 11-2009; 2053-2070
1666-6070
url http://hdl.handle.net/11336/17121
identifier_str_mv Kler, Pablo Alejandro; Guarnieri, Fabio Ariel; Berli, Claudio Luis Alberto; Simulation of 1d and 2d electrophoretic separations in microfluidics chips ; Asociación Argentina de Mecánica Computacional; Mecanica Computacional; XXVIII; 24; 11-2009; 2053-2070
1666-6070
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cimec.org.ar/ojs/index.php/mc/article/view/2859
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Mecánica Computacional
publisher.none.fl_str_mv Asociación Argentina de Mecánica Computacional
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083275693490176
score 13.22299

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