A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen

Autores
Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens; D. S.; Mora Garcia, Santiago; Yanovsky, Marcelo Javier; Weigl, B. H.; Yanovsky, J.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH-Lemos rapid test. Compared to the composite reference tests, the PATH-Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease.
Fil: Barfield, C. A.. PATH; Estados Unidos
Fil: Barney, R. S.. PATH; Estados Unidos
Fil: Crudder, C. H.. PATH; Estados Unidos
Fil: Wilmoth, J. L.. PATH; Estados Unidos
Fil: Stevens; D. S.. PATH; Estados Unidos
Fil: Mora Garcia, Santiago. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Weigl, B. H.. PATH; Estados Unidos
Fil: Yanovsky, J.. Laboratorio Lemos; Argentina
Materia
Chagas Disease
Immunoassay
Medical Diagnosis
Point of Care
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13720
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13720
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigenBarfield, C. A.Barney, R. S.Crudder, C. H.Wilmoth, J. L.Stevens; D. S.Mora Garcia, SantiagoYanovsky, Marcelo JavierWeigl, B. H.Yanovsky, J.Chagas DiseaseImmunoassayMedical DiagnosisPoint of Carehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH-Lemos rapid test. Compared to the composite reference tests, the PATH-Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease.Fil: Barfield, C. A.. PATH; Estados UnidosFil: Barney, R. S.. PATH; Estados UnidosFil: Crudder, C. H.. PATH; Estados UnidosFil: Wilmoth, J. L.. PATH; Estados UnidosFil: Stevens; D. S.. PATH; Estados UnidosFil: Mora Garcia, Santiago. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Weigl, B. H.. PATH; Estados UnidosFil: Yanovsky, J.. Laboratorio Lemos; ArgentinaInstitute Of Electrical And Electronics Engineers2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13720Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens; D. S.; et al.; A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen; Institute Of Electrical And Electronics Engineers; Ieee Transactions On Bio-medical Engineering; 58; 3; 3-2011; 814-8170018-9294enginfo:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/5601750/info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1109/TBME.2010.2087334info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T09:57:44Zoai:ri.conicet.gov.ar:11336/13720instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 09:57:44.989CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
title A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
spellingShingle A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
Barfield, C. A.
Chagas Disease
Immunoassay
Medical Diagnosis
Point of Care
title_short A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
title_full A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
title_fullStr A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
title_full_unstemmed A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
title_sort A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
dc.creator.none.fl_str_mv Barfield, C. A.
Barney, R. S.
Crudder, C. H.
Wilmoth, J. L.
Stevens; D. S.
Mora Garcia, Santiago
Yanovsky, Marcelo Javier
Weigl, B. H.
Yanovsky, J.
author Barfield, C. A.
author_facet Barfield, C. A.
Barney, R. S.
Crudder, C. H.
Wilmoth, J. L.
Stevens; D. S.
Mora Garcia, Santiago
Yanovsky, Marcelo Javier
Weigl, B. H.
Yanovsky, J.
author_role author
author2 Barney, R. S.
Crudder, C. H.
Wilmoth, J. L.
Stevens; D. S.
Mora Garcia, Santiago
Yanovsky, Marcelo Javier
Weigl, B. H.
Yanovsky, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Immunoassay
Medical Diagnosis
Point of Care
topic Chagas Disease
Immunoassay
Medical Diagnosis
Point of Care
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH-Lemos rapid test. Compared to the composite reference tests, the PATH-Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease.
Fil: Barfield, C. A.. PATH; Estados Unidos
Fil: Barney, R. S.. PATH; Estados Unidos
Fil: Crudder, C. H.. PATH; Estados Unidos
Fil: Wilmoth, J. L.. PATH; Estados Unidos
Fil: Stevens; D. S.. PATH; Estados Unidos
Fil: Mora Garcia, Santiago. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Weigl, B. H.. PATH; Estados Unidos
Fil: Yanovsky, J.. Laboratorio Lemos; Argentina
description Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH-Lemos rapid test. Compared to the composite reference tests, the PATH-Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13720
Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens; D. S.; et al.; A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen; Institute Of Electrical And Electronics Engineers; Ieee Transactions On Bio-medical Engineering; 58; 3; 3-2011; 814-817
0018-9294
url http://hdl.handle.net/11336/13720
identifier_str_mv Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens; D. S.; et al.; A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen; Institute Of Electrical And Electronics Engineers; Ieee Transactions On Bio-medical Engineering; 58; 3; 3-2011; 814-817
0018-9294
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ieeexplore.ieee.org/document/5601750/
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1109/TBME.2010.2087334
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institute Of Electrical And Electronics Engineers
publisher.none.fl_str_mv Institute Of Electrical And Electronics Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1858304754970525696
score 13.176822

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