Oxidative stress and lipolysis: new insights in fat metabolism

Autores
Funk, Melania Iara; Maniscalchi, Athina del Valle; Benzi Juncos, Oriana Nicole; Alza, Natalia Paola; Conde, Melisa Ailén; Salvador, Gabriela Alejandra; Uranga, Romina Maria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Prolonged oxidative stress (OS) directly affects fat metabolism, with implications in the onset of obesity, insulin resistance, and type 2 diabetes. Particularly in adipocytes, it is well known that OS participates in several mechanisms related with proliferation and differentiation. Our aim was to study the signaling events underlying lipolysis triggered by OS. For this purpose, we worked with different adipocyte in vitro cultures (differentiated 3T3L1 and mesenchymal stem cells) and with an in vivo model, all subjected to iron-induced OS. 3T3L1 adipocytes challenged with ferric ammonium citrate (FAC, 500-1000 µM) displayed augmented lipid peroxides and membrane permeability when compared with non-treated cells. The increase in OS markers observed in 3T3L1 adipocytes was coincident with a rise in glycerol release to the medium. These results were also corroborated in the in vivo model, where a decreased neutral lipid content in gonadal adipose tissue of iron-treated mice was observed. In addition, iron-treated animals presented a different architecture of gonadal fat characterized by cell shrinkage, decreased volume tissue, and fibrosis. Lipolysis in the white adipose tissue of humans and rodents is a step-wise process regulated by adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase. Exacerbated lipolysis was accompanied by the upregulation of βcatenin expression in 3T3L1 and in gonadal adipocytes. To ascertain the role of this signaling pathway in OS-induced lipolysis, we worked with adipocytes differentiated from primary mesenchymal stem cells with wild type expression or deletion of β-catenin gene. To this end, stem cells were isolated from outer ears of β-catenin fl/fl mice and after differentiation to adipocytes, gene deletion was induced with adenoviral Cre recombinase. The expression of the lipolytic enzymes, ATGL and HSL, was evaluated by qRT-PCR in wild type and β-catenin knock out (β-catenin KO) adipocytes exposed to vehicle or FAC. In wild type adipocytes, iron exposure increased ATGL and HSL mRNA levels, whereas lipolytic enzyme expression remained unchanged in β-catenin KO cells. Our results demonstrate that iron-induced OS is able to activate lipolysis through a mechanism involving the β-catenin pathway in fat cells.
Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
oxidative stress
fat metabolism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228468
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Oxidative stress and lipolysis: new insights in fat metabolismFunk, Melania IaraManiscalchi, Athina del ValleBenzi Juncos, Oriana NicoleAlza, Natalia PaolaConde, Melisa AilénSalvador, Gabriela AlejandraUranga, Romina Mariaoxidative stressfat metabolismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Prolonged oxidative stress (OS) directly affects fat metabolism, with implications in the onset of obesity, insulin resistance, and type 2 diabetes. Particularly in adipocytes, it is well known that OS participates in several mechanisms related with proliferation and differentiation. Our aim was to study the signaling events underlying lipolysis triggered by OS. For this purpose, we worked with different adipocyte in vitro cultures (differentiated 3T3L1 and mesenchymal stem cells) and with an in vivo model, all subjected to iron-induced OS. 3T3L1 adipocytes challenged with ferric ammonium citrate (FAC, 500-1000 µM) displayed augmented lipid peroxides and membrane permeability when compared with non-treated cells. The increase in OS markers observed in 3T3L1 adipocytes was coincident with a rise in glycerol release to the medium. These results were also corroborated in the in vivo model, where a decreased neutral lipid content in gonadal adipose tissue of iron-treated mice was observed. In addition, iron-treated animals presented a different architecture of gonadal fat characterized by cell shrinkage, decreased volume tissue, and fibrosis. Lipolysis in the white adipose tissue of humans and rodents is a step-wise process regulated by adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase. Exacerbated lipolysis was accompanied by the upregulation of βcatenin expression in 3T3L1 and in gonadal adipocytes. To ascertain the role of this signaling pathway in OS-induced lipolysis, we worked with adipocytes differentiated from primary mesenchymal stem cells with wild type expression or deletion of β-catenin gene. To this end, stem cells were isolated from outer ears of β-catenin fl/fl mice and after differentiation to adipocytes, gene deletion was induced with adenoviral Cre recombinase. The expression of the lipolytic enzymes, ATGL and HSL, was evaluated by qRT-PCR in wild type and β-catenin knock out (β-catenin KO) adipocytes exposed to vehicle or FAC. In wild type adipocytes, iron exposure increased ATGL and HSL mRNA levels, whereas lipolytic enzyme expression remained unchanged in β-catenin KO cells. Our results demonstrate that iron-induced OS is able to activate lipolysis through a mechanism involving the β-catenin pathway in fat cells.Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Maniscalchi, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularMendozaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science Press2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228468Oxidative stress and lipolysis: new insights in fat metabolism; LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mendoza; Argentina; 2022; 173-1730327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:10Zoai:ri.conicet.gov.ar:11336/228468instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:11.177CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative stress and lipolysis: new insights in fat metabolism
title Oxidative stress and lipolysis: new insights in fat metabolism
spellingShingle Oxidative stress and lipolysis: new insights in fat metabolism
Funk, Melania Iara
oxidative stress
fat metabolism
title_short Oxidative stress and lipolysis: new insights in fat metabolism
title_full Oxidative stress and lipolysis: new insights in fat metabolism
title_fullStr Oxidative stress and lipolysis: new insights in fat metabolism
title_full_unstemmed Oxidative stress and lipolysis: new insights in fat metabolism
title_sort Oxidative stress and lipolysis: new insights in fat metabolism
dc.creator.none.fl_str_mv Funk, Melania Iara
Maniscalchi, Athina del Valle
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author Funk, Melania Iara
author_facet Funk, Melania Iara
Maniscalchi, Athina del Valle
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author_role author
author2 Maniscalchi, Athina del Valle
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv oxidative stress
fat metabolism
topic oxidative stress
fat metabolism
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Prolonged oxidative stress (OS) directly affects fat metabolism, with implications in the onset of obesity, insulin resistance, and type 2 diabetes. Particularly in adipocytes, it is well known that OS participates in several mechanisms related with proliferation and differentiation. Our aim was to study the signaling events underlying lipolysis triggered by OS. For this purpose, we worked with different adipocyte in vitro cultures (differentiated 3T3L1 and mesenchymal stem cells) and with an in vivo model, all subjected to iron-induced OS. 3T3L1 adipocytes challenged with ferric ammonium citrate (FAC, 500-1000 µM) displayed augmented lipid peroxides and membrane permeability when compared with non-treated cells. The increase in OS markers observed in 3T3L1 adipocytes was coincident with a rise in glycerol release to the medium. These results were also corroborated in the in vivo model, where a decreased neutral lipid content in gonadal adipose tissue of iron-treated mice was observed. In addition, iron-treated animals presented a different architecture of gonadal fat characterized by cell shrinkage, decreased volume tissue, and fibrosis. Lipolysis in the white adipose tissue of humans and rodents is a step-wise process regulated by adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase. Exacerbated lipolysis was accompanied by the upregulation of βcatenin expression in 3T3L1 and in gonadal adipocytes. To ascertain the role of this signaling pathway in OS-induced lipolysis, we worked with adipocytes differentiated from primary mesenchymal stem cells with wild type expression or deletion of β-catenin gene. To this end, stem cells were isolated from outer ears of β-catenin fl/fl mice and after differentiation to adipocytes, gene deletion was induced with adenoviral Cre recombinase. The expression of the lipolytic enzymes, ATGL and HSL, was evaluated by qRT-PCR in wild type and β-catenin knock out (β-catenin KO) adipocytes exposed to vehicle or FAC. In wild type adipocytes, iron exposure increased ATGL and HSL mRNA levels, whereas lipolytic enzyme expression remained unchanged in β-catenin KO cells. Our results demonstrate that iron-induced OS is able to activate lipolysis through a mechanism involving the β-catenin pathway in fat cells.
Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Prolonged oxidative stress (OS) directly affects fat metabolism, with implications in the onset of obesity, insulin resistance, and type 2 diabetes. Particularly in adipocytes, it is well known that OS participates in several mechanisms related with proliferation and differentiation. Our aim was to study the signaling events underlying lipolysis triggered by OS. For this purpose, we worked with different adipocyte in vitro cultures (differentiated 3T3L1 and mesenchymal stem cells) and with an in vivo model, all subjected to iron-induced OS. 3T3L1 adipocytes challenged with ferric ammonium citrate (FAC, 500-1000 µM) displayed augmented lipid peroxides and membrane permeability when compared with non-treated cells. The increase in OS markers observed in 3T3L1 adipocytes was coincident with a rise in glycerol release to the medium. These results were also corroborated in the in vivo model, where a decreased neutral lipid content in gonadal adipose tissue of iron-treated mice was observed. In addition, iron-treated animals presented a different architecture of gonadal fat characterized by cell shrinkage, decreased volume tissue, and fibrosis. Lipolysis in the white adipose tissue of humans and rodents is a step-wise process regulated by adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase. Exacerbated lipolysis was accompanied by the upregulation of βcatenin expression in 3T3L1 and in gonadal adipocytes. To ascertain the role of this signaling pathway in OS-induced lipolysis, we worked with adipocytes differentiated from primary mesenchymal stem cells with wild type expression or deletion of β-catenin gene. To this end, stem cells were isolated from outer ears of β-catenin fl/fl mice and after differentiation to adipocytes, gene deletion was induced with adenoviral Cre recombinase. The expression of the lipolytic enzymes, ATGL and HSL, was evaluated by qRT-PCR in wild type and β-catenin knock out (β-catenin KO) adipocytes exposed to vehicle or FAC. In wild type adipocytes, iron exposure increased ATGL and HSL mRNA levels, whereas lipolytic enzyme expression remained unchanged in β-catenin KO cells. Our results demonstrate that iron-induced OS is able to activate lipolysis through a mechanism involving the β-catenin pathway in fat cells.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228468
Oxidative stress and lipolysis: new insights in fat metabolism; LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mendoza; Argentina; 2022; 173-173
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228468
identifier_str_mv Oxidative stress and lipolysis: new insights in fat metabolism; LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mendoza; Argentina; 2022; 173-173
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
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