Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats

Autores
Höcht, Christian; Opezzo, Javier A. W.; Taira, Carlos Alberto
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to demonstrate an alteration of the anterior hypothalamic catecholaminergic system in aortic coarctated (ACo) rats by the perfusion of β-adrenergic antagonist and the microinfusion of β-adrenergic agonist. Wistar urethane-chloralose anesthetized rats were used. The carotid artery was cannulated for blood pressure recording and changes in blood pressure were measured. A concentric microdialysis probe was inserted in the anterior hypothalamus. Metoprolol (a β1- adrenoceptor antagonist) perfusion (6μgml-1) reduced the mean arterial pressure (MAP) in the ACo rats but not in sham operated (SO) animals. The anterior hypothalamic infusion of non-specific β-adrenergic agonist isoproterenol induced a dose-dependent decrease of blood pressure in both experimental groups, but the depressor response was significantly lower in ACo rats. The pretreatment with atenolol, a selective β1- adrenoceptor antagonist, increased the depressor effect of isoproterenol in ACo rats, but not in SO rats. On the other hand, the hypotensive action of isoproterenol was significantly diminished after the administration of non-specific β-adrenoceptor antagonist propranolol in SO and ACo rats. The anterior hypothalamic infusion of clenbuterol, a selective β 2-adrenergic agonist, induced a dose-dependent decrease of blood pressure in both experimental groups. The depressor response to clenbuterol (1nmol) was significantly lower in ACo rats than in SO rats. In summary, this study provides the evidence that there is a β1-adrenergic compromise in anaesthetized ACo rats and this compromise may be involved in the maintenance of hypertension. On the other hand, this study also suggests the existence of pressor β1-adrenoceptors in the anterior hypothalamic area of ACo rats but not in SO rats. We also found a diminished depressor β2-adrenergic activity in ACo rats.
Fil: Höcht, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Opezzo, Javier A. W.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Taira, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
ANTERIOR HYPOTHALAMIC AREA
AORTIC COARCTATION
BLOOD PRESSURE
CLENBUTEROL
ISOPROTERENOL
METOPROLOL
REVERSE MICRODIALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162987
Acceder
id CONICETDig_1fa280c6e512f3ccf30357ea30e56e3f
oai_identifier_str oai:ri.conicet.gov.ar:11336/162987
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated ratsHöcht, ChristianOpezzo, Javier A. W.Taira, Carlos AlbertoANTERIOR HYPOTHALAMIC AREAAORTIC COARCTATIONBLOOD PRESSURECLENBUTEROLISOPROTERENOLMETOPROLOLREVERSE MICRODIALYSIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of this work was to demonstrate an alteration of the anterior hypothalamic catecholaminergic system in aortic coarctated (ACo) rats by the perfusion of β-adrenergic antagonist and the microinfusion of β-adrenergic agonist. Wistar urethane-chloralose anesthetized rats were used. The carotid artery was cannulated for blood pressure recording and changes in blood pressure were measured. A concentric microdialysis probe was inserted in the anterior hypothalamus. Metoprolol (a β1- adrenoceptor antagonist) perfusion (6μgml-1) reduced the mean arterial pressure (MAP) in the ACo rats but not in sham operated (SO) animals. The anterior hypothalamic infusion of non-specific β-adrenergic agonist isoproterenol induced a dose-dependent decrease of blood pressure in both experimental groups, but the depressor response was significantly lower in ACo rats. The pretreatment with atenolol, a selective β1- adrenoceptor antagonist, increased the depressor effect of isoproterenol in ACo rats, but not in SO rats. On the other hand, the hypotensive action of isoproterenol was significantly diminished after the administration of non-specific β-adrenoceptor antagonist propranolol in SO and ACo rats. The anterior hypothalamic infusion of clenbuterol, a selective β 2-adrenergic agonist, induced a dose-dependent decrease of blood pressure in both experimental groups. The depressor response to clenbuterol (1nmol) was significantly lower in ACo rats than in SO rats. In summary, this study provides the evidence that there is a β1-adrenergic compromise in anaesthetized ACo rats and this compromise may be involved in the maintenance of hypertension. On the other hand, this study also suggests the existence of pressor β1-adrenoceptors in the anterior hypothalamic area of ACo rats but not in SO rats. We also found a diminished depressor β2-adrenergic activity in ACo rats.Fil: Höcht, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Opezzo, Javier A. W.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Taira, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2004-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162987Höcht, Christian; Opezzo, Javier A. W.; Taira, Carlos Alberto; Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 49; 1; 1-2004; 17-211043-6618CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661803002536?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phrs.2003.07.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:53Zoai:ri.conicet.gov.ar:11336/162987instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:54.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
title Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
spellingShingle Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
Höcht, Christian
ANTERIOR HYPOTHALAMIC AREA
AORTIC COARCTATION
BLOOD PRESSURE
CLENBUTEROL
ISOPROTERENOL
METOPROLOL
REVERSE MICRODIALYSIS
title_short Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
title_full Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
title_fullStr Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
title_full_unstemmed Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
title_sort Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats
dc.creator.none.fl_str_mv Höcht, Christian
Opezzo, Javier A. W.
Taira, Carlos Alberto
author Höcht, Christian
author_facet Höcht, Christian
Opezzo, Javier A. W.
Taira, Carlos Alberto
author_role author
author2 Opezzo, Javier A. W.
Taira, Carlos Alberto
author2_role author
author
dc.subject.none.fl_str_mv ANTERIOR HYPOTHALAMIC AREA
AORTIC COARCTATION
BLOOD PRESSURE
CLENBUTEROL
ISOPROTERENOL
METOPROLOL
REVERSE MICRODIALYSIS
topic ANTERIOR HYPOTHALAMIC AREA
AORTIC COARCTATION
BLOOD PRESSURE
CLENBUTEROL
ISOPROTERENOL
METOPROLOL
REVERSE MICRODIALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to demonstrate an alteration of the anterior hypothalamic catecholaminergic system in aortic coarctated (ACo) rats by the perfusion of β-adrenergic antagonist and the microinfusion of β-adrenergic agonist. Wistar urethane-chloralose anesthetized rats were used. The carotid artery was cannulated for blood pressure recording and changes in blood pressure were measured. A concentric microdialysis probe was inserted in the anterior hypothalamus. Metoprolol (a β1- adrenoceptor antagonist) perfusion (6μgml-1) reduced the mean arterial pressure (MAP) in the ACo rats but not in sham operated (SO) animals. The anterior hypothalamic infusion of non-specific β-adrenergic agonist isoproterenol induced a dose-dependent decrease of blood pressure in both experimental groups, but the depressor response was significantly lower in ACo rats. The pretreatment with atenolol, a selective β1- adrenoceptor antagonist, increased the depressor effect of isoproterenol in ACo rats, but not in SO rats. On the other hand, the hypotensive action of isoproterenol was significantly diminished after the administration of non-specific β-adrenoceptor antagonist propranolol in SO and ACo rats. The anterior hypothalamic infusion of clenbuterol, a selective β 2-adrenergic agonist, induced a dose-dependent decrease of blood pressure in both experimental groups. The depressor response to clenbuterol (1nmol) was significantly lower in ACo rats than in SO rats. In summary, this study provides the evidence that there is a β1-adrenergic compromise in anaesthetized ACo rats and this compromise may be involved in the maintenance of hypertension. On the other hand, this study also suggests the existence of pressor β1-adrenoceptors in the anterior hypothalamic area of ACo rats but not in SO rats. We also found a diminished depressor β2-adrenergic activity in ACo rats.
Fil: Höcht, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Opezzo, Javier A. W.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Taira, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The aim of this work was to demonstrate an alteration of the anterior hypothalamic catecholaminergic system in aortic coarctated (ACo) rats by the perfusion of β-adrenergic antagonist and the microinfusion of β-adrenergic agonist. Wistar urethane-chloralose anesthetized rats were used. The carotid artery was cannulated for blood pressure recording and changes in blood pressure were measured. A concentric microdialysis probe was inserted in the anterior hypothalamus. Metoprolol (a β1- adrenoceptor antagonist) perfusion (6μgml-1) reduced the mean arterial pressure (MAP) in the ACo rats but not in sham operated (SO) animals. The anterior hypothalamic infusion of non-specific β-adrenergic agonist isoproterenol induced a dose-dependent decrease of blood pressure in both experimental groups, but the depressor response was significantly lower in ACo rats. The pretreatment with atenolol, a selective β1- adrenoceptor antagonist, increased the depressor effect of isoproterenol in ACo rats, but not in SO rats. On the other hand, the hypotensive action of isoproterenol was significantly diminished after the administration of non-specific β-adrenoceptor antagonist propranolol in SO and ACo rats. The anterior hypothalamic infusion of clenbuterol, a selective β 2-adrenergic agonist, induced a dose-dependent decrease of blood pressure in both experimental groups. The depressor response to clenbuterol (1nmol) was significantly lower in ACo rats than in SO rats. In summary, this study provides the evidence that there is a β1-adrenergic compromise in anaesthetized ACo rats and this compromise may be involved in the maintenance of hypertension. On the other hand, this study also suggests the existence of pressor β1-adrenoceptors in the anterior hypothalamic area of ACo rats but not in SO rats. We also found a diminished depressor β2-adrenergic activity in ACo rats.
publishDate 2004
dc.date.none.fl_str_mv 2004-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162987
Höcht, Christian; Opezzo, Javier A. W.; Taira, Carlos Alberto; Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 49; 1; 1-2004; 17-21
1043-6618
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162987
identifier_str_mv Höcht, Christian; Opezzo, Javier A. W.; Taira, Carlos Alberto; Anterior hypothalamic β-adrenergic activity in the maintenance of hypertension in aortic coarctated rats; Academic Press Ltd - Elsevier Science Ltd; Pharmacological Research; 49; 1; 1-2004; 17-21
1043-6618
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1043661803002536?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phrs.2003.07.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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