Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis

Autores
Salum, Maria Laura; Schmidt de León, Tobías; Erra Balsells, Rosa
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since the introduction of 3,5-dimethoxy-4-hydroxycinnamic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, the successful application of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) started. Cinnamics can exist as two different geometric isomers, the E- and Z-forms. The commercially available cinnamics currently used as matrices are E-cinnamics; they do not perform well in general for carbohydrate analysis. Recently, Z-cinnamic acid properties for matrices were studied and compared with those of the corresponding E-isomer. For the analysis of neutral/sulfated carbohydrates the outstanding performance for Z-SA was demonstrated. As the synthesis of pure Z-cinnamic acids requires several steps and the manipulation of some not friendly chemicals (i.e., bad smelling organic amines, toxic compounds, organic solvents, etc.), here we describe a convenient new one-pot protocol to prepare in situ, in a methanolic solution of commercial E-acid (i.e., E-SA), a Z- + E- mixture by photoisomerization (UVB irradiation); then, the only step required is the addition of water to the irradiated solution to become ready as a matrix stock solution for MALDI experiments. This "photo-made at home" matrix performs carbohydrate analysis with similar results to the corresponding Z-acid. The results here show that this novel protocol is a tool of choice for the direct, rapid and sensitive detection of neutral and sulfated carbohydrates without any tedious Z-cinnamic acid preparation and isolation.
Fil: Salum, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Schmidt de León, Tobías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
UV-MALDI-MS
carbohydrate
sinapinic acid
photochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182522
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/182522
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS AnalysisSalum, Maria LauraSchmidt de León, TobíasErra Balsells, RosaUV-MALDI-MScarbohydratesinapinic acidphotochemistryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Since the introduction of 3,5-dimethoxy-4-hydroxycinnamic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, the successful application of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) started. Cinnamics can exist as two different geometric isomers, the E- and Z-forms. The commercially available cinnamics currently used as matrices are E-cinnamics; they do not perform well in general for carbohydrate analysis. Recently, Z-cinnamic acid properties for matrices were studied and compared with those of the corresponding E-isomer. For the analysis of neutral/sulfated carbohydrates the outstanding performance for Z-SA was demonstrated. As the synthesis of pure Z-cinnamic acids requires several steps and the manipulation of some not friendly chemicals (i.e., bad smelling organic amines, toxic compounds, organic solvents, etc.), here we describe a convenient new one-pot protocol to prepare in situ, in a methanolic solution of commercial E-acid (i.e., E-SA), a Z- + E- mixture by photoisomerization (UVB irradiation); then, the only step required is the addition of water to the irradiated solution to become ready as a matrix stock solution for MALDI experiments. This "photo-made at home" matrix performs carbohydrate analysis with similar results to the corresponding Z-acid. The results here show that this novel protocol is a tool of choice for the direct, rapid and sensitive detection of neutral and sulfated carbohydrates without any tedious Z-cinnamic acid preparation and isolation.Fil: Salum, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Schmidt de León, Tobías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaRoyal Society of Chemistry2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182522Salum, Maria Laura; Schmidt de León, Tobías; Erra Balsells, Rosa; Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis; Royal Society of Chemistry; Analytical Methods; 7; 19; 8-2015; 8478-84831759-9679CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/C5AY01484Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:52Zoai:ri.conicet.gov.ar:11336/182522instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:53.022CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
title Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
spellingShingle Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
Salum, Maria Laura
UV-MALDI-MS
carbohydrate
sinapinic acid
photochemistry
title_short Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
title_full Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
title_fullStr Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
title_full_unstemmed Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
title_sort Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis
dc.creator.none.fl_str_mv Salum, Maria Laura
Schmidt de León, Tobías
Erra Balsells, Rosa
author Salum, Maria Laura
author_facet Salum, Maria Laura
Schmidt de León, Tobías
Erra Balsells, Rosa
author_role author
author2 Schmidt de León, Tobías
Erra Balsells, Rosa
author2_role author
author
dc.subject.none.fl_str_mv UV-MALDI-MS
carbohydrate
sinapinic acid
photochemistry
topic UV-MALDI-MS
carbohydrate
sinapinic acid
photochemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Since the introduction of 3,5-dimethoxy-4-hydroxycinnamic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, the successful application of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) started. Cinnamics can exist as two different geometric isomers, the E- and Z-forms. The commercially available cinnamics currently used as matrices are E-cinnamics; they do not perform well in general for carbohydrate analysis. Recently, Z-cinnamic acid properties for matrices were studied and compared with those of the corresponding E-isomer. For the analysis of neutral/sulfated carbohydrates the outstanding performance for Z-SA was demonstrated. As the synthesis of pure Z-cinnamic acids requires several steps and the manipulation of some not friendly chemicals (i.e., bad smelling organic amines, toxic compounds, organic solvents, etc.), here we describe a convenient new one-pot protocol to prepare in situ, in a methanolic solution of commercial E-acid (i.e., E-SA), a Z- + E- mixture by photoisomerization (UVB irradiation); then, the only step required is the addition of water to the irradiated solution to become ready as a matrix stock solution for MALDI experiments. This "photo-made at home" matrix performs carbohydrate analysis with similar results to the corresponding Z-acid. The results here show that this novel protocol is a tool of choice for the direct, rapid and sensitive detection of neutral and sulfated carbohydrates without any tedious Z-cinnamic acid preparation and isolation.
Fil: Salum, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Schmidt de León, Tobías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description Since the introduction of 3,5-dimethoxy-4-hydroxycinnamic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, the successful application of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) started. Cinnamics can exist as two different geometric isomers, the E- and Z-forms. The commercially available cinnamics currently used as matrices are E-cinnamics; they do not perform well in general for carbohydrate analysis. Recently, Z-cinnamic acid properties for matrices were studied and compared with those of the corresponding E-isomer. For the analysis of neutral/sulfated carbohydrates the outstanding performance for Z-SA was demonstrated. As the synthesis of pure Z-cinnamic acids requires several steps and the manipulation of some not friendly chemicals (i.e., bad smelling organic amines, toxic compounds, organic solvents, etc.), here we describe a convenient new one-pot protocol to prepare in situ, in a methanolic solution of commercial E-acid (i.e., E-SA), a Z- + E- mixture by photoisomerization (UVB irradiation); then, the only step required is the addition of water to the irradiated solution to become ready as a matrix stock solution for MALDI experiments. This "photo-made at home" matrix performs carbohydrate analysis with similar results to the corresponding Z-acid. The results here show that this novel protocol is a tool of choice for the direct, rapid and sensitive detection of neutral and sulfated carbohydrates without any tedious Z-cinnamic acid preparation and isolation.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182522
Salum, Maria Laura; Schmidt de León, Tobías; Erra Balsells, Rosa; Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis; Royal Society of Chemistry; Analytical Methods; 7; 19; 8-2015; 8478-8483
1759-9679
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182522
identifier_str_mv Salum, Maria Laura; Schmidt de León, Tobías; Erra Balsells, Rosa; Easy Protocol for Making at Home E-Sinapinic Acid Good Matrix for Neutral and Sulfated Carbohydrate MALDI-MS Analysis; Royal Society of Chemistry; Analytical Methods; 7; 19; 8-2015; 8478-8483
1759-9679
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/C5AY01484K
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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