Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients

Autores
Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; Pampena, María Betina; Huertas, Eduardo; Sánchez Loria, Fernando; Pairola, Alejandro; Bianchini, Michele; Mordoh, Jose; Levy, Estrella Mariel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.
Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Amat, Mora. Instituto Alexander Fleming; Argentina
Fil: Bruno, Luisina. Instituto Alexander Fleming; Argentina
Fil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Huertas, Eduardo. Instituto Alexander Fleming; Argentina
Fil: Sánchez Loria, Fernando. Instituto Alexander Fleming; Argentina
Fil: Pairola, Alejandro. Instituto Alexander Fleming; Argentina
Fil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Materia
Adcc
Cetuximab
Colorectal Cancer
Immune Supresion
Natural Killer Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7775
Acceder
id CONICETDig_1f85e831a8c3e31bc4b1b45ed4b0ead7
oai_identifier_str oai:ri.conicet.gov.ar:11336/7775
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patientsRocca, Yamila SolRoberti, Maria PaulaArriaga, Juan MartínAmat, MoraBruno, LuisinaPampena, María BetinaHuertas, EduardoSánchez Loria, FernandoPairola, AlejandroBianchini, MicheleMordoh, JoseLevy, Estrella MarielAdccCetuximabColorectal CancerImmune SupresionNatural Killer Cellshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Amat, Mora. Instituto Alexander Fleming; ArgentinaFil: Bruno, Luisina. Instituto Alexander Fleming; ArgentinaFil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Huertas, Eduardo. Instituto Alexander Fleming; ArgentinaFil: Sánchez Loria, Fernando. Instituto Alexander Fleming; ArgentinaFil: Pairola, Alejandro. Instituto Alexander Fleming; ArgentinaFil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaSage Publications Ltd2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7775Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-851753-4259enginfo:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/19/1/76.abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1177/1753425912453187info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:18Zoai:ri.conicet.gov.ar:11336/7775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:18.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
title Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
spellingShingle Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
Rocca, Yamila Sol
Adcc
Cetuximab
Colorectal Cancer
Immune Supresion
Natural Killer Cells
title_short Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
title_full Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
title_fullStr Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
title_full_unstemmed Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
title_sort Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
dc.creator.none.fl_str_mv Rocca, Yamila Sol
Roberti, Maria Paula
Arriaga, Juan Martín
Amat, Mora
Bruno, Luisina
Pampena, María Betina
Huertas, Eduardo
Sánchez Loria, Fernando
Pairola, Alejandro
Bianchini, Michele
Mordoh, Jose
Levy, Estrella Mariel
author Rocca, Yamila Sol
author_facet Rocca, Yamila Sol
Roberti, Maria Paula
Arriaga, Juan Martín
Amat, Mora
Bruno, Luisina
Pampena, María Betina
Huertas, Eduardo
Sánchez Loria, Fernando
Pairola, Alejandro
Bianchini, Michele
Mordoh, Jose
Levy, Estrella Mariel
author_role author
author2 Roberti, Maria Paula
Arriaga, Juan Martín
Amat, Mora
Bruno, Luisina
Pampena, María Betina
Huertas, Eduardo
Sánchez Loria, Fernando
Pairola, Alejandro
Bianchini, Michele
Mordoh, Jose
Levy, Estrella Mariel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adcc
Cetuximab
Colorectal Cancer
Immune Supresion
Natural Killer Cells
topic Adcc
Cetuximab
Colorectal Cancer
Immune Supresion
Natural Killer Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.
Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Amat, Mora. Instituto Alexander Fleming; Argentina
Fil: Bruno, Luisina. Instituto Alexander Fleming; Argentina
Fil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Huertas, Eduardo. Instituto Alexander Fleming; Argentina
Fil: Sánchez Loria, Fernando. Instituto Alexander Fleming; Argentina
Fil: Pairola, Alejandro. Instituto Alexander Fleming; Argentina
Fil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
description Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7775
Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-85
1753-4259
url http://hdl.handle.net/11336/7775
identifier_str_mv Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-85
1753-4259
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/19/1/76.abstract
info:eu-repo/semantics/altIdentifier/doi/10.1177/1753425912453187
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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