Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
- Autores
- Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; Pampena, María Betina; Huertas, Eduardo; Sánchez Loria, Fernando; Pairola, Alejandro; Bianchini, Michele; Mordoh, Jose; Levy, Estrella Mariel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.
Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Amat, Mora. Instituto Alexander Fleming; Argentina
Fil: Bruno, Luisina. Instituto Alexander Fleming; Argentina
Fil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Huertas, Eduardo. Instituto Alexander Fleming; Argentina
Fil: Sánchez Loria, Fernando. Instituto Alexander Fleming; Argentina
Fil: Pairola, Alejandro. Instituto Alexander Fleming; Argentina
Fil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina - Materia
-
Adcc
Cetuximab
Colorectal Cancer
Immune Supresion
Natural Killer Cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/7775
Ver los metadatos del registro completo
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spelling |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patientsRocca, Yamila SolRoberti, Maria PaulaArriaga, Juan MartínAmat, MoraBruno, LuisinaPampena, María BetinaHuertas, EduardoSánchez Loria, FernandoPairola, AlejandroBianchini, MicheleMordoh, JoseLevy, Estrella MarielAdccCetuximabColorectal CancerImmune SupresionNatural Killer Cellshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer.Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Amat, Mora. Instituto Alexander Fleming; ArgentinaFil: Bruno, Luisina. Instituto Alexander Fleming; ArgentinaFil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Huertas, Eduardo. Instituto Alexander Fleming; ArgentinaFil: Sánchez Loria, Fernando. Instituto Alexander Fleming; ArgentinaFil: Pairola, Alejandro. Instituto Alexander Fleming; ArgentinaFil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; ArgentinaSage Publications Ltd2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7775Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-851753-4259enginfo:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/19/1/76.abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1177/1753425912453187info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:02Zoai:ri.conicet.gov.ar:11336/7775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:03.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
title |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
spellingShingle |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients Rocca, Yamila Sol Adcc Cetuximab Colorectal Cancer Immune Supresion Natural Killer Cells |
title_short |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
title_full |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
title_fullStr |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
title_full_unstemmed |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
title_sort |
Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients |
dc.creator.none.fl_str_mv |
Rocca, Yamila Sol Roberti, Maria Paula Arriaga, Juan Martín Amat, Mora Bruno, Luisina Pampena, María Betina Huertas, Eduardo Sánchez Loria, Fernando Pairola, Alejandro Bianchini, Michele Mordoh, Jose Levy, Estrella Mariel |
author |
Rocca, Yamila Sol |
author_facet |
Rocca, Yamila Sol Roberti, Maria Paula Arriaga, Juan Martín Amat, Mora Bruno, Luisina Pampena, María Betina Huertas, Eduardo Sánchez Loria, Fernando Pairola, Alejandro Bianchini, Michele Mordoh, Jose Levy, Estrella Mariel |
author_role |
author |
author2 |
Roberti, Maria Paula Arriaga, Juan Martín Amat, Mora Bruno, Luisina Pampena, María Betina Huertas, Eduardo Sánchez Loria, Fernando Pairola, Alejandro Bianchini, Michele Mordoh, Jose Levy, Estrella Mariel |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Adcc Cetuximab Colorectal Cancer Immune Supresion Natural Killer Cells |
topic |
Adcc Cetuximab Colorectal Cancer Immune Supresion Natural Killer Cells |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer. Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Amat, Mora. Instituto Alexander Fleming; Argentina Fil: Bruno, Luisina. Instituto Alexander Fleming; Argentina Fil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Huertas, Eduardo. Instituto Alexander Fleming; Argentina Fil: Sánchez Loria, Fernando. Instituto Alexander Fleming; Argentina Fil: Pairola, Alejandro. Instituto Alexander Fleming; Argentina Fil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina |
description |
Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/7775 Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-85 1753-4259 |
url |
http://hdl.handle.net/11336/7775 |
identifier_str_mv |
Rocca, Yamila Sol; Roberti, Maria Paula; Arriaga, Juan Martín; Amat, Mora; Bruno, Luisina; et al.; Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients; Sage Publications Ltd; Innate Immunity; 19; 1; 2-2013; 76-85 1753-4259 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/19/1/76.abstract info:eu-repo/semantics/altIdentifier/doi/10.1177/1753425912453187 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sage Publications Ltd |
publisher.none.fl_str_mv |
Sage Publications Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |