Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
- Autores
- Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.
Fil: Ramírez-Vélez, Robinson.
Fil: Bustamante, Juanit.
Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;
Fil: Aguilar de Plata, Ana.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular; - Materia
-
HUMAN PLACENTA
EXCERCISE TRAINING
NITRIC OXIDE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/566
Ver los metadatos del registro completo
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Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.Ramírez-Vélez, RobinsonBustamante, JuanitCzerniczyniec, AnaliaAguilar de Plata, AnaLores Arnaiz, SilviaHUMAN PLACENTAEXCERCISE TRAININGNITRIC OXIDEhttps://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.Fil: Ramírez-Vélez, Robinson.Fil: Bustamante, Juanit.Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;Fil: Aguilar de Plata, Ana.Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;Public Library Science2013-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/566Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828218/pdf/pone.0080225.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:23Zoai:ri.conicet.gov.ar:11336/566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:24.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
title |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
spellingShingle |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. Ramírez-Vélez, Robinson HUMAN PLACENTA EXCERCISE TRAINING NITRIC OXIDE |
title_short |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
title_full |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
title_fullStr |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
title_full_unstemmed |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
title_sort |
Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta. |
dc.creator.none.fl_str_mv |
Ramírez-Vélez, Robinson Bustamante, Juanit Czerniczyniec, Analia Aguilar de Plata, Ana Lores Arnaiz, Silvia |
author |
Ramírez-Vélez, Robinson |
author_facet |
Ramírez-Vélez, Robinson Bustamante, Juanit Czerniczyniec, Analia Aguilar de Plata, Ana Lores Arnaiz, Silvia |
author_role |
author |
author2 |
Bustamante, Juanit Czerniczyniec, Analia Aguilar de Plata, Ana Lores Arnaiz, Silvia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
HUMAN PLACENTA EXCERCISE TRAINING NITRIC OXIDE |
topic |
HUMAN PLACENTA EXCERCISE TRAINING NITRIC OXIDE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.2 |
dc.description.none.fl_txt_mv |
Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. Fil: Ramírez-Vélez, Robinson. Fil: Bustamante, Juanit. Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular; Fil: Aguilar de Plata, Ana. Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular; |
description |
Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-14 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/566 Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7; 1932-6203 |
url |
http://hdl.handle.net/11336/566 |
identifier_str_mv |
Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7; 1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828218/pdf/pone.0080225.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614276146266112 |
score |
13.070432 |