Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.

Autores
Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.
Fil: Ramírez-Vélez, Robinson.
Fil: Bustamante, Juanit.
Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;
Fil: Aguilar de Plata, Ana.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;
Materia
HUMAN PLACENTA
EXCERCISE TRAINING
NITRIC OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/566

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oai_identifier_str oai:ri.conicet.gov.ar:11336/566
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network_name_str CONICET Digital (CONICET)
spelling Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.Ramírez-Vélez, RobinsonBustamante, JuanitCzerniczyniec, AnaliaAguilar de Plata, AnaLores Arnaiz, SilviaHUMAN PLACENTAEXCERCISE TRAININGNITRIC OXIDEhttps://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.Fil: Ramírez-Vélez, Robinson.Fil: Bustamante, Juanit.Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;Fil: Aguilar de Plata, Ana.Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;Public Library Science2013-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/566Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828218/pdf/pone.0080225.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:23Zoai:ri.conicet.gov.ar:11336/566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:24.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
title Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
spellingShingle Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
Ramírez-Vélez, Robinson
HUMAN PLACENTA
EXCERCISE TRAINING
NITRIC OXIDE
title_short Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
title_full Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
title_fullStr Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
title_full_unstemmed Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
title_sort Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.
dc.creator.none.fl_str_mv Ramírez-Vélez, Robinson
Bustamante, Juanit
Czerniczyniec, Analia
Aguilar de Plata, Ana
Lores Arnaiz, Silvia
author Ramírez-Vélez, Robinson
author_facet Ramírez-Vélez, Robinson
Bustamante, Juanit
Czerniczyniec, Analia
Aguilar de Plata, Ana
Lores Arnaiz, Silvia
author_role author
author2 Bustamante, Juanit
Czerniczyniec, Analia
Aguilar de Plata, Ana
Lores Arnaiz, Silvia
author2_role author
author
author
author
dc.subject.none.fl_str_mv HUMAN PLACENTA
EXCERCISE TRAINING
NITRIC OXIDE
topic HUMAN PLACENTA
EXCERCISE TRAINING
NITRIC OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
dc.description.none.fl_txt_mv Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.
Fil: Ramírez-Vélez, Robinson.
Fil: Bustamante, Juanit.
Fil: Czerniczyniec, Analia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;
Fil: Aguilar de Plata, Ana.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Bioquimica y Medicina Molecular;
description Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16?20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55?75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1?3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/566
Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7;
1932-6203
url http://hdl.handle.net/11336/566
identifier_str_mv Ramírez-Vélez, Robinson; Bustamante, Juanit; Czerniczyniec, Analia; Aguilar de Plata, Ana; Lores Arnaiz, Silvia; Effect of Exercise Training on eNnos Expression, NO Production and Oxygen Metabolism in Human Placenta.; Public Library Science; Plos One; 8; 11; 14-11-2013; 1-7;
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828218/pdf/pone.0080225.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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