Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects

Autores
Garcés, Marisa Elisabeth; Sequeiros, Cynthia; Olivera, Nelda Lila
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. The aim of this study was to assess the probiotic properties and the protective mode of action of Lactobacillus pentosus H16 against V. alginolyticus 03/8525, through in vitro and in vivo studies using Artemia franciscana. This strain showed antimicrobial activity against V. alginolyticus 03/8525 and Aeromonas salmonicida subsp. salmonicida ATCC33658 possibly related with lactobacilli organic acid production. It was able to survive at high rainbow trout bile concentrations and showed high selective adhesion to rainbow trout mucus (1.2 E+05 ± 8.0 E+03 cells/cm2). H16 outcompeted with V. alginolyticus 03/8525 and A. salmonicida subsp. salmonicida ATCC33658, greatly reducing their adherence to rainbow trout mucus (64.8 and 74.1%, respectively). Moreover, H16 produced a cell-bound biosurfactant which caused an important diminution of the surface tension. H16 also protected Artemia nauplii against mortality when it was administered previously to V. alginolyticus 03/8525 inoculation. Furthermore, H16 bioencapsulated in Artemia suggesting that it is possible to use live carriers in its administration. We conclude that the capability of L. pentosus H16 to selectively adheres to mucosal surfaces and produces cell-bound biosurfactants, displacing pathogenic strains, in addition to its antimicrobial activity, confer H16 competitive advantages against pathogens as demonstrated in vivo challenge experiments. Thus, L. pentosus H16, a marine bacterium from the intestinal tract of hake, is an interesting probiotic for Artemia culture and has also potential in the prevention of vibriosis in other aquaculture activities such as larvae culture and fish farming.
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Materia
Probiotic
Artemia
Vibriosis
Lactobacillus Pentosus
Bioencapsulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21467
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effectsGarcés, Marisa ElisabethSequeiros, CynthiaOlivera, Nelda LilaProbioticArtemiaVibriosisLactobacillus PentosusBioencapsulationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. The aim of this study was to assess the probiotic properties and the protective mode of action of Lactobacillus pentosus H16 against V. alginolyticus 03/8525, through in vitro and in vivo studies using Artemia franciscana. This strain showed antimicrobial activity against V. alginolyticus 03/8525 and Aeromonas salmonicida subsp. salmonicida ATCC33658 possibly related with lactobacilli organic acid production. It was able to survive at high rainbow trout bile concentrations and showed high selective adhesion to rainbow trout mucus (1.2 E+05 ± 8.0 E+03 cells/cm2). H16 outcompeted with V. alginolyticus 03/8525 and A. salmonicida subsp. salmonicida ATCC33658, greatly reducing their adherence to rainbow trout mucus (64.8 and 74.1%, respectively). Moreover, H16 produced a cell-bound biosurfactant which caused an important diminution of the surface tension. H16 also protected Artemia nauplii against mortality when it was administered previously to V. alginolyticus 03/8525 inoculation. Furthermore, H16 bioencapsulated in Artemia suggesting that it is possible to use live carriers in its administration. We conclude that the capability of L. pentosus H16 to selectively adheres to mucosal surfaces and produces cell-bound biosurfactants, displacing pathogenic strains, in addition to its antimicrobial activity, confer H16 competitive advantages against pathogens as demonstrated in vivo challenge experiments. Thus, L. pentosus H16, a marine bacterium from the intestinal tract of hake, is an interesting probiotic for Artemia culture and has also potential in the prevention of vibriosis in other aquaculture activities such as larvae culture and fish farming.Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaInter-Research2015-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21467Garcés, Marisa Elisabeth; Sequeiros, Cynthia; Olivera, Nelda Lila; Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects; Inter-Research; Diseases Of Aquatic Organisms; 113; 1; 10-2-2015; 41-500177-51031616-1580CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3354/dao02815info:eu-repo/semantics/altIdentifier/url/http://www.int-res.com/abstracts/dao/v113/n1/p41-50/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-05-06T16:22:28Zoai:ri.conicet.gov.ar:11336/21467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-05-06 16:22:28.79CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
title Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
spellingShingle Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
Garcés, Marisa Elisabeth
Probiotic
Artemia
Vibriosis
Lactobacillus Pentosus
Bioencapsulation
title_short Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
title_full Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
title_fullStr Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
title_full_unstemmed Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
title_sort Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects
dc.creator.none.fl_str_mv Garcés, Marisa Elisabeth
Sequeiros, Cynthia
Olivera, Nelda Lila
author Garcés, Marisa Elisabeth
author_facet Garcés, Marisa Elisabeth
Sequeiros, Cynthia
Olivera, Nelda Lila
author_role author
author2 Sequeiros, Cynthia
Olivera, Nelda Lila
author2_role author
author
dc.subject.none.fl_str_mv Probiotic
Artemia
Vibriosis
Lactobacillus Pentosus
Bioencapsulation
topic Probiotic
Artemia
Vibriosis
Lactobacillus Pentosus
Bioencapsulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. The aim of this study was to assess the probiotic properties and the protective mode of action of Lactobacillus pentosus H16 against V. alginolyticus 03/8525, through in vitro and in vivo studies using Artemia franciscana. This strain showed antimicrobial activity against V. alginolyticus 03/8525 and Aeromonas salmonicida subsp. salmonicida ATCC33658 possibly related with lactobacilli organic acid production. It was able to survive at high rainbow trout bile concentrations and showed high selective adhesion to rainbow trout mucus (1.2 E+05 ± 8.0 E+03 cells/cm2). H16 outcompeted with V. alginolyticus 03/8525 and A. salmonicida subsp. salmonicida ATCC33658, greatly reducing their adherence to rainbow trout mucus (64.8 and 74.1%, respectively). Moreover, H16 produced a cell-bound biosurfactant which caused an important diminution of the surface tension. H16 also protected Artemia nauplii against mortality when it was administered previously to V. alginolyticus 03/8525 inoculation. Furthermore, H16 bioencapsulated in Artemia suggesting that it is possible to use live carriers in its administration. We conclude that the capability of L. pentosus H16 to selectively adheres to mucosal surfaces and produces cell-bound biosurfactants, displacing pathogenic strains, in addition to its antimicrobial activity, confer H16 competitive advantages against pathogens as demonstrated in vivo challenge experiments. Thus, L. pentosus H16, a marine bacterium from the intestinal tract of hake, is an interesting probiotic for Artemia culture and has also potential in the prevention of vibriosis in other aquaculture activities such as larvae culture and fish farming.
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
description Vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. The aim of this study was to assess the probiotic properties and the protective mode of action of Lactobacillus pentosus H16 against V. alginolyticus 03/8525, through in vitro and in vivo studies using Artemia franciscana. This strain showed antimicrobial activity against V. alginolyticus 03/8525 and Aeromonas salmonicida subsp. salmonicida ATCC33658 possibly related with lactobacilli organic acid production. It was able to survive at high rainbow trout bile concentrations and showed high selective adhesion to rainbow trout mucus (1.2 E+05 ± 8.0 E+03 cells/cm2). H16 outcompeted with V. alginolyticus 03/8525 and A. salmonicida subsp. salmonicida ATCC33658, greatly reducing their adherence to rainbow trout mucus (64.8 and 74.1%, respectively). Moreover, H16 produced a cell-bound biosurfactant which caused an important diminution of the surface tension. H16 also protected Artemia nauplii against mortality when it was administered previously to V. alginolyticus 03/8525 inoculation. Furthermore, H16 bioencapsulated in Artemia suggesting that it is possible to use live carriers in its administration. We conclude that the capability of L. pentosus H16 to selectively adheres to mucosal surfaces and produces cell-bound biosurfactants, displacing pathogenic strains, in addition to its antimicrobial activity, confer H16 competitive advantages against pathogens as demonstrated in vivo challenge experiments. Thus, L. pentosus H16, a marine bacterium from the intestinal tract of hake, is an interesting probiotic for Artemia culture and has also potential in the prevention of vibriosis in other aquaculture activities such as larvae culture and fish farming.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21467
Garcés, Marisa Elisabeth; Sequeiros, Cynthia; Olivera, Nelda Lila; Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects; Inter-Research; Diseases Of Aquatic Organisms; 113; 1; 10-2-2015; 41-50
0177-5103
1616-1580
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21467
identifier_str_mv Garcés, Marisa Elisabeth; Sequeiros, Cynthia; Olivera, Nelda Lila; Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects; Inter-Research; Diseases Of Aquatic Organisms; 113; 1; 10-2-2015; 41-50
0177-5103
1616-1580
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3354/dao02815
info:eu-repo/semantics/altIdentifier/url/http://www.int-res.com/abstracts/dao/v113/n1/p41-50/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inter-Research
publisher.none.fl_str_mv Inter-Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.637756

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