Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus
- Autores
- Romanutti, Carina; D'antuono, Alejandra Lorena; Palacios, Carlos Adolfo; Quattrocchi, Valeria; Zamorano, Patricia Ines; la Torre, Jose Leonardo; Mattion, Nora Marta
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of the present study was to assess the effect of introducing a priming step with replication-defective viral vectors encoding the capsid proteins of FMDV, followed by a boost with killed virus vaccines, using a suitable BALB/c mice model. Additionally, the immune response to other combined vector immunization regimens was studied. For this purpose, we analyzed different prime-boost immunizations with recombinant adenovirus (Ad), herpesvirus amplicons (Hs) and/or killed virus (KV) vaccines. The highest antibody titers were found in the group that received two doses of adjuvanted KV (P < 0.002). Antibody titers were higher in those groups receiving a mixed regimen of vectors, compared to immunization with either vector alone (P < 0.0001). Priming with any of the viral vectors induced a shift of the cytokine balance toward a Th1 type immune response regardless of the delivery system used for boosting. The highest IgG1 titer was induced by two doses of adjuvanted KV (P = 0.0002) and the highest IgG2a titer corresponded to the group primed with Ad and boosted with KV (P = 0.01). Re-stimulation of all groups of mice with 0.5 mg of inactivated virus five months later resulted in a fast increase of antibody titers in all the groups tested. After virus stimulation, antibody titers in the groups that received KV alone or Ad prime-KV boost, were indistinguishable (P = 0.800). Protection from challenge was similar (75%) in the groups of animals that received Ad prime-Hs boost or Ad prime-KV boost, or two doses of oil-adjuvanted KV. The data presented in this study suggest that sequential immunization with viral vectors-based vaccines combined with protein-based vaccines have the potential to enhance the quality of the immune response against FMDV.
Fil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;
Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina;
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina;
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina;
Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; - Materia
-
Fmdv
Prime-Boost Regimens
Adenovirus Vectors
Herpervirus Amplicons
Marker Vaccines - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1678
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Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virusRomanutti, CarinaD'antuono, Alejandra LorenaPalacios, Carlos AdolfoQuattrocchi, ValeriaZamorano, Patricia Inesla Torre, Jose LeonardoMattion, Nora MartaFmdvPrime-Boost RegimensAdenovirus VectorsHerpervirus AmpliconsMarker Vaccineshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to assess the effect of introducing a priming step with replication-defective viral vectors encoding the capsid proteins of FMDV, followed by a boost with killed virus vaccines, using a suitable BALB/c mice model. Additionally, the immune response to other combined vector immunization regimens was studied. For this purpose, we analyzed different prime-boost immunizations with recombinant adenovirus (Ad), herpesvirus amplicons (Hs) and/or killed virus (KV) vaccines. The highest antibody titers were found in the group that received two doses of adjuvanted KV (P < 0.002). Antibody titers were higher in those groups receiving a mixed regimen of vectors, compared to immunization with either vector alone (P < 0.0001). Priming with any of the viral vectors induced a shift of the cytokine balance toward a Th1 type immune response regardless of the delivery system used for boosting. The highest IgG1 titer was induced by two doses of adjuvanted KV (P = 0.0002) and the highest IgG2a titer corresponded to the group primed with Ad and boosted with KV (P = 0.01). Re-stimulation of all groups of mice with 0.5 mg of inactivated virus five months later resulted in a fast increase of antibody titers in all the groups tested. After virus stimulation, antibody titers in the groups that received KV alone or Ad prime-KV boost, were indistinguishable (P = 0.800). Protection from challenge was similar (75%) in the groups of animals that received Ad prime-Hs boost or Ad prime-KV boost, or two doses of oil-adjuvanted KV. The data presented in this study suggest that sequential immunization with viral vectors-based vaccines combined with protein-based vaccines have the potential to enhance the quality of the immune response against FMDV.Fil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina;Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina;Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina;Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina;Elsevier2013-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1678Romanutti, Carina; D'antuono, Alejandra Lorena; Palacios, Carlos Adolfo; Quattrocchi, Valeria; Zamorano, Patricia Ines; et al.; Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus; Elsevier; Veterinary Microbiology; 165; 3-4; 30-8-2013; 333-3400378-1135enginfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.vetmic.2013.04.017info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/?term=Evaluation+of+the+immune+response+elicited+by+vaccination+with+viral+vectors+encoding+FMDV+capsid+proteins+and+boosted+with+inactivated+virusinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:32Zoai:ri.conicet.gov.ar:11336/1678instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:33.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
title |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
spellingShingle |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus Romanutti, Carina Fmdv Prime-Boost Regimens Adenovirus Vectors Herpervirus Amplicons Marker Vaccines |
title_short |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
title_full |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
title_fullStr |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
title_full_unstemmed |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
title_sort |
Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus |
dc.creator.none.fl_str_mv |
Romanutti, Carina D'antuono, Alejandra Lorena Palacios, Carlos Adolfo Quattrocchi, Valeria Zamorano, Patricia Ines la Torre, Jose Leonardo Mattion, Nora Marta |
author |
Romanutti, Carina |
author_facet |
Romanutti, Carina D'antuono, Alejandra Lorena Palacios, Carlos Adolfo Quattrocchi, Valeria Zamorano, Patricia Ines la Torre, Jose Leonardo Mattion, Nora Marta |
author_role |
author |
author2 |
D'antuono, Alejandra Lorena Palacios, Carlos Adolfo Quattrocchi, Valeria Zamorano, Patricia Ines la Torre, Jose Leonardo Mattion, Nora Marta |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Fmdv Prime-Boost Regimens Adenovirus Vectors Herpervirus Amplicons Marker Vaccines |
topic |
Fmdv Prime-Boost Regimens Adenovirus Vectors Herpervirus Amplicons Marker Vaccines |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The aim of the present study was to assess the effect of introducing a priming step with replication-defective viral vectors encoding the capsid proteins of FMDV, followed by a boost with killed virus vaccines, using a suitable BALB/c mice model. Additionally, the immune response to other combined vector immunization regimens was studied. For this purpose, we analyzed different prime-boost immunizations with recombinant adenovirus (Ad), herpesvirus amplicons (Hs) and/or killed virus (KV) vaccines. The highest antibody titers were found in the group that received two doses of adjuvanted KV (P < 0.002). Antibody titers were higher in those groups receiving a mixed regimen of vectors, compared to immunization with either vector alone (P < 0.0001). Priming with any of the viral vectors induced a shift of the cytokine balance toward a Th1 type immune response regardless of the delivery system used for boosting. The highest IgG1 titer was induced by two doses of adjuvanted KV (P = 0.0002) and the highest IgG2a titer corresponded to the group primed with Ad and boosted with KV (P = 0.01). Re-stimulation of all groups of mice with 0.5 mg of inactivated virus five months later resulted in a fast increase of antibody titers in all the groups tested. After virus stimulation, antibody titers in the groups that received KV alone or Ad prime-KV boost, were indistinguishable (P = 0.800). Protection from challenge was similar (75%) in the groups of animals that received Ad prime-Hs boost or Ad prime-KV boost, or two doses of oil-adjuvanted KV. The data presented in this study suggest that sequential immunization with viral vectors-based vaccines combined with protein-based vaccines have the potential to enhance the quality of the immune response against FMDV. Fil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina; Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Instituto Nacional de Tecnologia Agropecuaria; Argentina; Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina; Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina; |
description |
The aim of the present study was to assess the effect of introducing a priming step with replication-defective viral vectors encoding the capsid proteins of FMDV, followed by a boost with killed virus vaccines, using a suitable BALB/c mice model. Additionally, the immune response to other combined vector immunization regimens was studied. For this purpose, we analyzed different prime-boost immunizations with recombinant adenovirus (Ad), herpesvirus amplicons (Hs) and/or killed virus (KV) vaccines. The highest antibody titers were found in the group that received two doses of adjuvanted KV (P < 0.002). Antibody titers were higher in those groups receiving a mixed regimen of vectors, compared to immunization with either vector alone (P < 0.0001). Priming with any of the viral vectors induced a shift of the cytokine balance toward a Th1 type immune response regardless of the delivery system used for boosting. The highest IgG1 titer was induced by two doses of adjuvanted KV (P = 0.0002) and the highest IgG2a titer corresponded to the group primed with Ad and boosted with KV (P = 0.01). Re-stimulation of all groups of mice with 0.5 mg of inactivated virus five months later resulted in a fast increase of antibody titers in all the groups tested. After virus stimulation, antibody titers in the groups that received KV alone or Ad prime-KV boost, were indistinguishable (P = 0.800). Protection from challenge was similar (75%) in the groups of animals that received Ad prime-Hs boost or Ad prime-KV boost, or two doses of oil-adjuvanted KV. The data presented in this study suggest that sequential immunization with viral vectors-based vaccines combined with protein-based vaccines have the potential to enhance the quality of the immune response against FMDV. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-30 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1678 Romanutti, Carina; D'antuono, Alejandra Lorena; Palacios, Carlos Adolfo; Quattrocchi, Valeria; Zamorano, Patricia Ines; et al.; Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus; Elsevier; Veterinary Microbiology; 165; 3-4; 30-8-2013; 333-340 0378-1135 |
url |
http://hdl.handle.net/11336/1678 |
identifier_str_mv |
Romanutti, Carina; D'antuono, Alejandra Lorena; Palacios, Carlos Adolfo; Quattrocchi, Valeria; Zamorano, Patricia Ines; et al.; Evaluation of the immune response elicited by vaccination with viral vectors encoding FMDV capsid proteins and boosted with inactivated virus; Elsevier; Veterinary Microbiology; 165; 3-4; 30-8-2013; 333-340 0378-1135 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.vetmic.2013.04.017 info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/?term=Evaluation+of+the+immune+response+elicited+by+vaccination+with+viral+vectors+encoding+FMDV+capsid+proteins+and+boosted+with+inactivated+virus |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |