Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity

Autores
Fragoso, Gladis; Esquivel Guadarrama, Fernando; Santana, Angélica; Bobes, Raúl J.; Hernández, Beatriz; Cervantes, Jacquelynne; Segura, René; Goldbaum, Fernando Alberto; Sciutto, Edda; Rosas, Gabriela
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through the mucosal system. This study was designed to explore the immunogenic properties of the Taenia crassiceps protective peptide GK-1 administered orally. Mice were orally immunized with the synthetic GK-1 peptide in its linear form with or without the Brucella lumazine synthase (BLS) protein adjuvant or as a chimera recombinantly bound to BLS (BLS-GK-1). Mice were boosted twice with GK-1 only at 15-day intervals. A significant rate of protection of 64.7% was achieved in GK-1-immunized mice, and that rate significantly increased to 91.8 and 96% when mice were primed with GK-1 coadministered with BLS as an adjuvant and BLS as a carrier, respectively. Specific antibodies and T cell activation and proliferation accompanied the protection induced, revealing the potent immunogenicity of GK-1. Through immunohistochemical studies, GK-1 was detected in T and B cell zones of the Peyer's patches (PP) and mesenteric lymph nodes. In the latter, abundant proliferating cells were detected by 5'-bromo-2'-deoxyuridine incorporation. No proliferation was detected in PP. Altogether, these results portray the potent immunogenic properties of GK-1 administered orally and reinforce the usefulness of BLS as an adjuvant and adequate vaccine delivery system for oral vaccines.
Fil: Fragoso, Gladis. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Esquivel Guadarrama, Fernando. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; México
Fil: Santana, Angélica. Universidad Autónoma del Estado de Morelos; México
Fil: Bobes, Raúl J.. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Hernández, Beatriz. Universidad Nacional Autónoma de México. Facultad de Medicina; México
Fil: Cervantes, Jacquelynne. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Segura, René. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Sciutto, Edda. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Rosas, Gabriela. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; México
Materia
Cysticercosis
Prime Boost
Bls
Immunity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12835
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12835
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunityFragoso, GladisEsquivel Guadarrama, FernandoSantana, AngélicaBobes, Raúl J.Hernández, BeatrizCervantes, JacquelynneSegura, RenéGoldbaum, Fernando AlbertoSciutto, EddaRosas, GabrielaCysticercosisPrime BoostBlsImmunityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through the mucosal system. This study was designed to explore the immunogenic properties of the Taenia crassiceps protective peptide GK-1 administered orally. Mice were orally immunized with the synthetic GK-1 peptide in its linear form with or without the Brucella lumazine synthase (BLS) protein adjuvant or as a chimera recombinantly bound to BLS (BLS-GK-1). Mice were boosted twice with GK-1 only at 15-day intervals. A significant rate of protection of 64.7% was achieved in GK-1-immunized mice, and that rate significantly increased to 91.8 and 96% when mice were primed with GK-1 coadministered with BLS as an adjuvant and BLS as a carrier, respectively. Specific antibodies and T cell activation and proliferation accompanied the protection induced, revealing the potent immunogenicity of GK-1. Through immunohistochemical studies, GK-1 was detected in T and B cell zones of the Peyer's patches (PP) and mesenteric lymph nodes. In the latter, abundant proliferating cells were detected by 5'-bromo-2'-deoxyuridine incorporation. No proliferation was detected in PP. Altogether, these results portray the potent immunogenic properties of GK-1 administered orally and reinforce the usefulness of BLS as an adjuvant and adequate vaccine delivery system for oral vaccines.Fil: Fragoso, Gladis. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; MéxicoFil: Esquivel Guadarrama, Fernando. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; MéxicoFil: Santana, Angélica. Universidad Autónoma del Estado de Morelos; MéxicoFil: Bobes, Raúl J.. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; MéxicoFil: Hernández, Beatriz. Universidad Nacional Autónoma de México. Facultad de Medicina; MéxicoFil: Cervantes, Jacquelynne. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; MéxicoFil: Segura, René. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; MéxicoFil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Sciutto, Edda. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; MéxicoFil: Rosas, Gabriela. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; MéxicoAmerican Society For Microbiology2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12835Fragoso, Gladis; Esquivel Guadarrama, Fernando; Santana, Angélica; Bobes, Raúl J.; Hernández, Beatriz; et al.; Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity; American Society For Microbiology; Clinical And Vaccine Immunology; 18; 7; 2011; 1067-10761556-6811enginfo:eu-repo/semantics/altIdentifier/url/http://cvi.asm.org/content/18/7/1067.longinfo:eu-repo/semantics/altIdentifier/doi/10.1128/CVI.05030-11info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147315/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:48Zoai:ri.conicet.gov.ar:11336/12835instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:48.437CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
title Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
spellingShingle Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
Fragoso, Gladis
Cysticercosis
Prime Boost
Bls
Immunity
title_short Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
title_full Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
title_fullStr Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
title_full_unstemmed Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
title_sort Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity
dc.creator.none.fl_str_mv Fragoso, Gladis
Esquivel Guadarrama, Fernando
Santana, Angélica
Bobes, Raúl J.
Hernández, Beatriz
Cervantes, Jacquelynne
Segura, René
Goldbaum, Fernando Alberto
Sciutto, Edda
Rosas, Gabriela
author Fragoso, Gladis
author_facet Fragoso, Gladis
Esquivel Guadarrama, Fernando
Santana, Angélica
Bobes, Raúl J.
Hernández, Beatriz
Cervantes, Jacquelynne
Segura, René
Goldbaum, Fernando Alberto
Sciutto, Edda
Rosas, Gabriela
author_role author
author2 Esquivel Guadarrama, Fernando
Santana, Angélica
Bobes, Raúl J.
Hernández, Beatriz
Cervantes, Jacquelynne
Segura, René
Goldbaum, Fernando Alberto
Sciutto, Edda
Rosas, Gabriela
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cysticercosis
Prime Boost
Bls
Immunity
topic Cysticercosis
Prime Boost
Bls
Immunity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through the mucosal system. This study was designed to explore the immunogenic properties of the Taenia crassiceps protective peptide GK-1 administered orally. Mice were orally immunized with the synthetic GK-1 peptide in its linear form with or without the Brucella lumazine synthase (BLS) protein adjuvant or as a chimera recombinantly bound to BLS (BLS-GK-1). Mice were boosted twice with GK-1 only at 15-day intervals. A significant rate of protection of 64.7% was achieved in GK-1-immunized mice, and that rate significantly increased to 91.8 and 96% when mice were primed with GK-1 coadministered with BLS as an adjuvant and BLS as a carrier, respectively. Specific antibodies and T cell activation and proliferation accompanied the protection induced, revealing the potent immunogenicity of GK-1. Through immunohistochemical studies, GK-1 was detected in T and B cell zones of the Peyer's patches (PP) and mesenteric lymph nodes. In the latter, abundant proliferating cells were detected by 5'-bromo-2'-deoxyuridine incorporation. No proliferation was detected in PP. Altogether, these results portray the potent immunogenic properties of GK-1 administered orally and reinforce the usefulness of BLS as an adjuvant and adequate vaccine delivery system for oral vaccines.
Fil: Fragoso, Gladis. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Esquivel Guadarrama, Fernando. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; México
Fil: Santana, Angélica. Universidad Autónoma del Estado de Morelos; México
Fil: Bobes, Raúl J.. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Hernández, Beatriz. Universidad Nacional Autónoma de México. Facultad de Medicina; México
Fil: Cervantes, Jacquelynne. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Segura, René. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Sciutto, Edda. Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas; México
Fil: Rosas, Gabriela. Universidad Autónoma del Estado de Morelos. Facultad de Medicina; México
description Oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through the mucosal system. This study was designed to explore the immunogenic properties of the Taenia crassiceps protective peptide GK-1 administered orally. Mice were orally immunized with the synthetic GK-1 peptide in its linear form with or without the Brucella lumazine synthase (BLS) protein adjuvant or as a chimera recombinantly bound to BLS (BLS-GK-1). Mice were boosted twice with GK-1 only at 15-day intervals. A significant rate of protection of 64.7% was achieved in GK-1-immunized mice, and that rate significantly increased to 91.8 and 96% when mice were primed with GK-1 coadministered with BLS as an adjuvant and BLS as a carrier, respectively. Specific antibodies and T cell activation and proliferation accompanied the protection induced, revealing the potent immunogenicity of GK-1. Through immunohistochemical studies, GK-1 was detected in T and B cell zones of the Peyer's patches (PP) and mesenteric lymph nodes. In the latter, abundant proliferating cells were detected by 5'-bromo-2'-deoxyuridine incorporation. No proliferation was detected in PP. Altogether, these results portray the potent immunogenic properties of GK-1 administered orally and reinforce the usefulness of BLS as an adjuvant and adequate vaccine delivery system for oral vaccines.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12835
Fragoso, Gladis; Esquivel Guadarrama, Fernando; Santana, Angélica; Bobes, Raúl J.; Hernández, Beatriz; et al.; Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity; American Society For Microbiology; Clinical And Vaccine Immunology; 18; 7; 2011; 1067-1076
1556-6811
url http://hdl.handle.net/11336/12835
identifier_str_mv Fragoso, Gladis; Esquivel Guadarrama, Fernando; Santana, Angélica; Bobes, Raúl J.; Hernández, Beatriz; et al.; Heterologous prime-boost oral immunization with GK-1 peptide from Taenia crassiceps cysticerci induces protective immunity; American Society For Microbiology; Clinical And Vaccine Immunology; 18; 7; 2011; 1067-1076
1556-6811
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://cvi.asm.org/content/18/7/1067.long
info:eu-repo/semantics/altIdentifier/doi/10.1128/CVI.05030-11
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147315/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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