Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice

Autores
Muhammad, Imani; Cremonini, Eleonora; Mathieu, Patricia Andrea; Adamo, Ana María; Oteiza, Patricia Isabel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Obesity and consumption of high-fat diets (HFD) are associated with intestinal permeabilization and increased paracellulartransport of endotoxins, which can promote neuroinflammation. Inflammation can affect the hypothalamic pituitary adrenal (HPA) axis,which controls responses to stress and downregulates the brain-derived neurotrophic factor (BDNF), which can promote anxiety anddepression, conditions frequently found in obesity. We previously showed that consumption of anthocyanins (AC) mitigate HFD-inducedinsulin resistance, intestinal permeability, and inflammation.Objectives: This study investigated if a dietary supplementation with a cyanidin- and delphinidin-rich extract (CDRE) could counteractHFD/obesity-induced hippocampal inflammation in mice.Methods: C57BL/6J male mice were fed for 14 wk on one of the following diets: 1) a control diet containing 10% total calories from fat (C),2) a control diet supplemented with 40 mg AC/kg body weight (BW) (CAC), 3) a HFD containing 60% total calories from fat (lard) (HF), or4) the HFD supplemented with 2, 20, or 40 mg AC/kg BW (HFA2, HFA20, and HFA40, respectively). In plasma and in the hippocampus,parameters of neuroinflammation and the underlying cause (endotoxemia) and consequences (alterations to the HPA and BDNF downregulation)were measured.Results: Consumption of the HFD caused endotoxemia. Accordingly, hippocampal Tlr4 mRNA levels were 110% higher in the HF group,which were both prevented by CDRE supplementation. Consumption of the HFD also caused: 1) microgliosis and increased expression ofgenes involved in neuroinflammation, that is, Iba-1, Nox4, Tnfα, and Il-1β, 2) alterations of HPA axis regulation, that is, with low expressionof mineralocorticoid (MR) and glucocorticoid (GR) receptors; and 3) decreased Bdnf expression. Supplementation of HFD-fed mice withCDRE mitigated neuroinflammation, microgliosis, and MR and BDNF decreases.Conclusions: CDRE supplementation mitigates the negative effects associated with HFD consumption and obesity in mouse hippocampus,in part by decreasing inflammation, improving glucocorticoid metabolism, and upregulating BDNF.
Fil: Muhammad, Imani. University of California; Estados Unidos
Fil: Cremonini, Eleonora. University of California; Estados Unidos
Fil: Mathieu, Patricia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Oteiza, Patricia Isabel. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
high-fat diet
anthocyanidins
BDNF
neuroinflammation
endotoxemia
hippocampus, HPA axis
C57BL/6J mice
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263424
Acceder
id CONICETDig_1e70f74388693fe5c889b556ed4fd12a
oai_identifier_str oai:ri.conicet.gov.ar:11336/263424
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in MiceMuhammad, ImaniCremonini, EleonoraMathieu, Patricia AndreaAdamo, Ana MaríaOteiza, Patricia Isabelhigh-fat dietanthocyanidinsBDNFneuroinflammationendotoxemiahippocampus, HPA axisC57BL/6J micehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Obesity and consumption of high-fat diets (HFD) are associated with intestinal permeabilization and increased paracellulartransport of endotoxins, which can promote neuroinflammation. Inflammation can affect the hypothalamic pituitary adrenal (HPA) axis,which controls responses to stress and downregulates the brain-derived neurotrophic factor (BDNF), which can promote anxiety anddepression, conditions frequently found in obesity. We previously showed that consumption of anthocyanins (AC) mitigate HFD-inducedinsulin resistance, intestinal permeability, and inflammation.Objectives: This study investigated if a dietary supplementation with a cyanidin- and delphinidin-rich extract (CDRE) could counteractHFD/obesity-induced hippocampal inflammation in mice.Methods: C57BL/6J male mice were fed for 14 wk on one of the following diets: 1) a control diet containing 10% total calories from fat (C),2) a control diet supplemented with 40 mg AC/kg body weight (BW) (CAC), 3) a HFD containing 60% total calories from fat (lard) (HF), or4) the HFD supplemented with 2, 20, or 40 mg AC/kg BW (HFA2, HFA20, and HFA40, respectively). In plasma and in the hippocampus,parameters of neuroinflammation and the underlying cause (endotoxemia) and consequences (alterations to the HPA and BDNF downregulation)were measured.Results: Consumption of the HFD caused endotoxemia. Accordingly, hippocampal Tlr4 mRNA levels were 110% higher in the HF group,which were both prevented by CDRE supplementation. Consumption of the HFD also caused: 1) microgliosis and increased expression ofgenes involved in neuroinflammation, that is, Iba-1, Nox4, Tnfα, and Il-1β, 2) alterations of HPA axis regulation, that is, with low expressionof mineralocorticoid (MR) and glucocorticoid (GR) receptors; and 3) decreased Bdnf expression. Supplementation of HFD-fed mice withCDRE mitigated neuroinflammation, microgliosis, and MR and BDNF decreases.Conclusions: CDRE supplementation mitigates the negative effects associated with HFD consumption and obesity in mouse hippocampus,in part by decreasing inflammation, improving glucocorticoid metabolism, and upregulating BDNF.Fil: Muhammad, Imani. University of California; Estados UnidosFil: Cremonini, Eleonora. University of California; Estados UnidosFil: Mathieu, Patricia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Oteiza, Patricia Isabel. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaAmer Soc Nutritional Science2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263424Muhammad, Imani; Cremonini, Eleonora; Mathieu, Patricia Andrea; Adamo, Ana María; Oteiza, Patricia Isabel; Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice; Amer Soc Nutritional Science; Journal Of Nutrition; 154; 9; 9-2024; 2752-27620022-3166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0022316624004097info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tjnut.2024.07.028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:07Zoai:ri.conicet.gov.ar:11336/263424instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:07.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
title Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
spellingShingle Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
Muhammad, Imani
high-fat diet
anthocyanidins
BDNF
neuroinflammation
endotoxemia
hippocampus, HPA axis
C57BL/6J mice
title_short Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
title_full Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
title_fullStr Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
title_full_unstemmed Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
title_sort Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice
dc.creator.none.fl_str_mv Muhammad, Imani
Cremonini, Eleonora
Mathieu, Patricia Andrea
Adamo, Ana María
Oteiza, Patricia Isabel
author Muhammad, Imani
author_facet Muhammad, Imani
Cremonini, Eleonora
Mathieu, Patricia Andrea
Adamo, Ana María
Oteiza, Patricia Isabel
author_role author
author2 Cremonini, Eleonora
Mathieu, Patricia Andrea
Adamo, Ana María
Oteiza, Patricia Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv high-fat diet
anthocyanidins
BDNF
neuroinflammation
endotoxemia
hippocampus, HPA axis
C57BL/6J mice
topic high-fat diet
anthocyanidins
BDNF
neuroinflammation
endotoxemia
hippocampus, HPA axis
C57BL/6J mice
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Obesity and consumption of high-fat diets (HFD) are associated with intestinal permeabilization and increased paracellulartransport of endotoxins, which can promote neuroinflammation. Inflammation can affect the hypothalamic pituitary adrenal (HPA) axis,which controls responses to stress and downregulates the brain-derived neurotrophic factor (BDNF), which can promote anxiety anddepression, conditions frequently found in obesity. We previously showed that consumption of anthocyanins (AC) mitigate HFD-inducedinsulin resistance, intestinal permeability, and inflammation.Objectives: This study investigated if a dietary supplementation with a cyanidin- and delphinidin-rich extract (CDRE) could counteractHFD/obesity-induced hippocampal inflammation in mice.Methods: C57BL/6J male mice were fed for 14 wk on one of the following diets: 1) a control diet containing 10% total calories from fat (C),2) a control diet supplemented with 40 mg AC/kg body weight (BW) (CAC), 3) a HFD containing 60% total calories from fat (lard) (HF), or4) the HFD supplemented with 2, 20, or 40 mg AC/kg BW (HFA2, HFA20, and HFA40, respectively). In plasma and in the hippocampus,parameters of neuroinflammation and the underlying cause (endotoxemia) and consequences (alterations to the HPA and BDNF downregulation)were measured.Results: Consumption of the HFD caused endotoxemia. Accordingly, hippocampal Tlr4 mRNA levels were 110% higher in the HF group,which were both prevented by CDRE supplementation. Consumption of the HFD also caused: 1) microgliosis and increased expression ofgenes involved in neuroinflammation, that is, Iba-1, Nox4, Tnfα, and Il-1β, 2) alterations of HPA axis regulation, that is, with low expressionof mineralocorticoid (MR) and glucocorticoid (GR) receptors; and 3) decreased Bdnf expression. Supplementation of HFD-fed mice withCDRE mitigated neuroinflammation, microgliosis, and MR and BDNF decreases.Conclusions: CDRE supplementation mitigates the negative effects associated with HFD consumption and obesity in mouse hippocampus,in part by decreasing inflammation, improving glucocorticoid metabolism, and upregulating BDNF.
Fil: Muhammad, Imani. University of California; Estados Unidos
Fil: Cremonini, Eleonora. University of California; Estados Unidos
Fil: Mathieu, Patricia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Adamo, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Oteiza, Patricia Isabel. University of California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Background: Obesity and consumption of high-fat diets (HFD) are associated with intestinal permeabilization and increased paracellulartransport of endotoxins, which can promote neuroinflammation. Inflammation can affect the hypothalamic pituitary adrenal (HPA) axis,which controls responses to stress and downregulates the brain-derived neurotrophic factor (BDNF), which can promote anxiety anddepression, conditions frequently found in obesity. We previously showed that consumption of anthocyanins (AC) mitigate HFD-inducedinsulin resistance, intestinal permeability, and inflammation.Objectives: This study investigated if a dietary supplementation with a cyanidin- and delphinidin-rich extract (CDRE) could counteractHFD/obesity-induced hippocampal inflammation in mice.Methods: C57BL/6J male mice were fed for 14 wk on one of the following diets: 1) a control diet containing 10% total calories from fat (C),2) a control diet supplemented with 40 mg AC/kg body weight (BW) (CAC), 3) a HFD containing 60% total calories from fat (lard) (HF), or4) the HFD supplemented with 2, 20, or 40 mg AC/kg BW (HFA2, HFA20, and HFA40, respectively). In plasma and in the hippocampus,parameters of neuroinflammation and the underlying cause (endotoxemia) and consequences (alterations to the HPA and BDNF downregulation)were measured.Results: Consumption of the HFD caused endotoxemia. Accordingly, hippocampal Tlr4 mRNA levels were 110% higher in the HF group,which were both prevented by CDRE supplementation. Consumption of the HFD also caused: 1) microgliosis and increased expression ofgenes involved in neuroinflammation, that is, Iba-1, Nox4, Tnfα, and Il-1β, 2) alterations of HPA axis regulation, that is, with low expressionof mineralocorticoid (MR) and glucocorticoid (GR) receptors; and 3) decreased Bdnf expression. Supplementation of HFD-fed mice withCDRE mitigated neuroinflammation, microgliosis, and MR and BDNF decreases.Conclusions: CDRE supplementation mitigates the negative effects associated with HFD consumption and obesity in mouse hippocampus,in part by decreasing inflammation, improving glucocorticoid metabolism, and upregulating BDNF.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263424
Muhammad, Imani; Cremonini, Eleonora; Mathieu, Patricia Andrea; Adamo, Ana María; Oteiza, Patricia Isabel; Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice; Amer Soc Nutritional Science; Journal Of Nutrition; 154; 9; 9-2024; 2752-2762
0022-3166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263424
identifier_str_mv Muhammad, Imani; Cremonini, Eleonora; Mathieu, Patricia Andrea; Adamo, Ana María; Oteiza, Patricia Isabel; Dietary Anthocyanins Mitigate High-Fat Diet-Induced Hippocampal Inflammation in Mice; Amer Soc Nutritional Science; Journal Of Nutrition; 154; 9; 9-2024; 2752-2762
0022-3166
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0022316624004097
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tjnut.2024.07.028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Amer Soc Nutritional Science
publisher.none.fl_str_mv Amer Soc Nutritional Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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