A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes

Autores
Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; Ruiz, Andrea; Perez Vidakovics, Maria Laura Anabella; Benveniste, Patricia; Galkin, Oleksandr; Zuñiga Pflucker, Juan Carlos; Serra, Pau
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity. We confirmed expression of EVA1 on cortical and medullary epithelial cell subsets and revealed a restricted pattern of expression on CD4- CD8- double negative (DN) cell subsets, with the highest level of expression on DN3 (CD44lowCD25+) thymocytes. G9P3-1MAb is a valuable reagent to study thymic T cell development and is likely useful for the analysis of pathological conditions affecting thymopoiesis, such as thymic involution caused by stress or aging.
Fil: Garabatos, Nahir. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Blanco, Jesus. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Fandos, Cesar. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Lopez, Elena. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Santamaria, Pere. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. University of Calgary; Canadá
Fil: Ruiz, Andrea. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Perez Vidakovics, Maria Laura Anabella. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benveniste, Patricia. University of Toronto; Canadá
Fil: Galkin, Oleksandr. University of Toronto; Canadá
Fil: Zuñiga Pflucker, Juan Carlos. University of Toronto; Canadá
Fil: Serra, Pau. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Materia
Eva1
Thymopoiesis
Double Negative Cell Subset
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41512
Acceder
id CONICETDig_1ddd79ebaeeeed43c014c1838687c4f0
oai_identifier_str oai:ri.conicet.gov.ar:11336/41512
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- ThymocytesGarabatos, NahirBlanco, JesusFandos, CesarLopez, ElenaSantamaria, PereRuiz, AndreaPerez Vidakovics, Maria Laura AnabellaBenveniste, PatriciaGalkin, OleksandrZuñiga Pflucker, Juan CarlosSerra, PauEva1ThymopoiesisDouble Negative Cell Subsethttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity. We confirmed expression of EVA1 on cortical and medullary epithelial cell subsets and revealed a restricted pattern of expression on CD4- CD8- double negative (DN) cell subsets, with the highest level of expression on DN3 (CD44lowCD25+) thymocytes. G9P3-1MAb is a valuable reagent to study thymic T cell development and is likely useful for the analysis of pathological conditions affecting thymopoiesis, such as thymic involution caused by stress or aging.Fil: Garabatos, Nahir. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaFil: Blanco, Jesus. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaFil: Fandos, Cesar. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaFil: Lopez, Elena. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaFil: Santamaria, Pere. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. University of Calgary; CanadáFil: Ruiz, Andrea. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaFil: Perez Vidakovics, Maria Laura Anabella. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benveniste, Patricia. University of Toronto; CanadáFil: Galkin, Oleksandr. University of Toronto; CanadáFil: Zuñiga Pflucker, Juan Carlos. University of Toronto; CanadáFil: Serra, Pau. Institut d'Investigacions Biomèdiques August Pi i Sunyer; EspañaMary Ann Liebert2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41512Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; et al.; A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes; Mary Ann Liebert; Monoclonal Antibodies in Immunodiagnosis and Immunotherapy; 33; 5; 10-2014; 305-3112167-9436CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/mab.2014.0030info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/abs/10.1089/mab.2014.0030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:50Zoai:ri.conicet.gov.ar:11336/41512instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:51.233CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
title A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
spellingShingle A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
Garabatos, Nahir
Eva1
Thymopoiesis
Double Negative Cell Subset
title_short A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
title_full A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
title_fullStr A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
title_full_unstemmed A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
title_sort A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes
dc.creator.none.fl_str_mv Garabatos, Nahir
Blanco, Jesus
Fandos, Cesar
Lopez, Elena
Santamaria, Pere
Ruiz, Andrea
Perez Vidakovics, Maria Laura Anabella
Benveniste, Patricia
Galkin, Oleksandr
Zuñiga Pflucker, Juan Carlos
Serra, Pau
author Garabatos, Nahir
author_facet Garabatos, Nahir
Blanco, Jesus
Fandos, Cesar
Lopez, Elena
Santamaria, Pere
Ruiz, Andrea
Perez Vidakovics, Maria Laura Anabella
Benveniste, Patricia
Galkin, Oleksandr
Zuñiga Pflucker, Juan Carlos
Serra, Pau
author_role author
author2 Blanco, Jesus
Fandos, Cesar
Lopez, Elena
Santamaria, Pere
Ruiz, Andrea
Perez Vidakovics, Maria Laura Anabella
Benveniste, Patricia
Galkin, Oleksandr
Zuñiga Pflucker, Juan Carlos
Serra, Pau
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Eva1
Thymopoiesis
Double Negative Cell Subset
topic Eva1
Thymopoiesis
Double Negative Cell Subset
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity. We confirmed expression of EVA1 on cortical and medullary epithelial cell subsets and revealed a restricted pattern of expression on CD4- CD8- double negative (DN) cell subsets, with the highest level of expression on DN3 (CD44lowCD25+) thymocytes. G9P3-1MAb is a valuable reagent to study thymic T cell development and is likely useful for the analysis of pathological conditions affecting thymopoiesis, such as thymic involution caused by stress or aging.
Fil: Garabatos, Nahir. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Blanco, Jesus. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Fandos, Cesar. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Lopez, Elena. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Santamaria, Pere. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. University of Calgary; Canadá
Fil: Ruiz, Andrea. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
Fil: Perez Vidakovics, Maria Laura Anabella. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benveniste, Patricia. University of Toronto; Canadá
Fil: Galkin, Oleksandr. University of Toronto; Canadá
Fil: Zuñiga Pflucker, Juan Carlos. University of Toronto; Canadá
Fil: Serra, Pau. Institut d'Investigacions Biomèdiques August Pi i Sunyer; España
description Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity. We confirmed expression of EVA1 on cortical and medullary epithelial cell subsets and revealed a restricted pattern of expression on CD4- CD8- double negative (DN) cell subsets, with the highest level of expression on DN3 (CD44lowCD25+) thymocytes. G9P3-1MAb is a valuable reagent to study thymic T cell development and is likely useful for the analysis of pathological conditions affecting thymopoiesis, such as thymic involution caused by stress or aging.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41512
Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; et al.; A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes; Mary Ann Liebert; Monoclonal Antibodies in Immunodiagnosis and Immunotherapy; 33; 5; 10-2014; 305-311
2167-9436
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41512
identifier_str_mv Garabatos, Nahir; Blanco, Jesus; Fandos, Cesar; Lopez, Elena; Santamaria, Pere; et al.; A Monoclonal Antibody Against the Extracellular Domain of Mouse and Human Epithelial V-like Antigen 1 Reveals a Restricted Expression Pattern Among CD4- CD8- Thymocytes; Mary Ann Liebert; Monoclonal Antibodies in Immunodiagnosis and Immunotherapy; 33; 5; 10-2014; 305-311
2167-9436
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/mab.2014.0030
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/abs/10.1089/mab.2014.0030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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