Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest

Autores
Hanisch, Priscila Elena; Lavinia Oblanca, Pablo Damián; Suarez, Andrew; Lijtmaer, Dario Alejandro; Leponce, Maurice; Paris, Carolina Ivon; Tubaro, Pablo Luis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding patterns of species diversity rely on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the southernmost extent of this forest. We assessed ant diversity using both cytochrome c oxidase subunit 1 (COI) sequences and traditional morphological approaches and then compared the results of these two methods. We successfully obtained COI sequences for 312 specimens belonging to 124 species, providing a DNA barcode reference library for nearly 50% of the currently known ant fauna of INP. Our results evidenced a clear barcode gap for all but two species, with a mean intraspecific divergence of 0.72%, and an average congeneric distance of 17.25%. Congruently, the library assembled here was remarkably useful for the discrimination of the ants of INP and even allowed us to link unidentified males and queens to their worker castes. To detect overlooked diversity, we classified the DNA barcodes into Molecular Operational Taxonomic Units (MOTUs) using three different clustering algorithms and then compared their number and composition to that of reference species identified based on morphology. The MOTU count was always higher than that of reference species regardless of the method, suggesting that the diversity of ants at INP could be between 6% and 10% higher than currently recognized. Lastly, our survey contributed with 78 new barcode clusters to the global DNA barcode reference library, and added 36 new records of ant species for the INP, being 23 of them new citations for Argentina.
Fil: Hanisch, Priscila Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Lavinia Oblanca, Pablo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Suarez, Andrew. University of Illinois; Estados Unidos
Fil: Lijtmaer, Dario Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Leponce, Maurice. Royal Belgian Institute of Natural Sciences; Bélgica
Fil: Paris, Carolina Ivon. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Tubaro, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Materia
ARGENTINA
IGUAZU NATIONAL PARK
DNA BARCODING
FORMICIDAE
SPECIES DELIMITATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48318
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic ForestHanisch, Priscila ElenaLavinia Oblanca, Pablo DamiánSuarez, AndrewLijtmaer, Dario AlejandroLeponce, MauriceParis, Carolina IvonTubaro, Pablo LuisARGENTINAIGUAZU NATIONAL PARKDNA BARCODINGFORMICIDAESPECIES DELIMITATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Understanding patterns of species diversity rely on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the southernmost extent of this forest. We assessed ant diversity using both cytochrome c oxidase subunit 1 (COI) sequences and traditional morphological approaches and then compared the results of these two methods. We successfully obtained COI sequences for 312 specimens belonging to 124 species, providing a DNA barcode reference library for nearly 50% of the currently known ant fauna of INP. Our results evidenced a clear barcode gap for all but two species, with a mean intraspecific divergence of 0.72%, and an average congeneric distance of 17.25%. Congruently, the library assembled here was remarkably useful for the discrimination of the ants of INP and even allowed us to link unidentified males and queens to their worker castes. To detect overlooked diversity, we classified the DNA barcodes into Molecular Operational Taxonomic Units (MOTUs) using three different clustering algorithms and then compared their number and composition to that of reference species identified based on morphology. The MOTU count was always higher than that of reference species regardless of the method, suggesting that the diversity of ants at INP could be between 6% and 10% higher than currently recognized. Lastly, our survey contributed with 78 new barcode clusters to the global DNA barcode reference library, and added 36 new records of ant species for the INP, being 23 of them new citations for Argentina.Fil: Hanisch, Priscila Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Lavinia Oblanca, Pablo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Suarez, Andrew. University of Illinois; Estados UnidosFil: Lijtmaer, Dario Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Leponce, Maurice. Royal Belgian Institute of Natural Sciences; BélgicaFil: Paris, Carolina Ivon. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Tubaro, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaWiley Blackwell Publishing, Inc2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48318Hanisch, Priscila Elena; Lavinia Oblanca, Pablo Damián; Suarez, Andrew; Lijtmaer, Dario Alejandro; Leponce, Maurice; et al.; Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest; Wiley Blackwell Publishing, Inc; Ecology and Evolution; 7; 23; 12-2017; 10451-104662045-7758CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.3549info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.3549info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:01Zoai:ri.conicet.gov.ar:11336/48318instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:01.774CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
title Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
spellingShingle Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
Hanisch, Priscila Elena
ARGENTINA
IGUAZU NATIONAL PARK
DNA BARCODING
FORMICIDAE
SPECIES DELIMITATION
title_short Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
title_full Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
title_fullStr Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
title_full_unstemmed Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
title_sort Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest
dc.creator.none.fl_str_mv Hanisch, Priscila Elena
Lavinia Oblanca, Pablo Damián
Suarez, Andrew
Lijtmaer, Dario Alejandro
Leponce, Maurice
Paris, Carolina Ivon
Tubaro, Pablo Luis
author Hanisch, Priscila Elena
author_facet Hanisch, Priscila Elena
Lavinia Oblanca, Pablo Damián
Suarez, Andrew
Lijtmaer, Dario Alejandro
Leponce, Maurice
Paris, Carolina Ivon
Tubaro, Pablo Luis
author_role author
author2 Lavinia Oblanca, Pablo Damián
Suarez, Andrew
Lijtmaer, Dario Alejandro
Leponce, Maurice
Paris, Carolina Ivon
Tubaro, Pablo Luis
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
IGUAZU NATIONAL PARK
DNA BARCODING
FORMICIDAE
SPECIES DELIMITATION
topic ARGENTINA
IGUAZU NATIONAL PARK
DNA BARCODING
FORMICIDAE
SPECIES DELIMITATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Understanding patterns of species diversity rely on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the southernmost extent of this forest. We assessed ant diversity using both cytochrome c oxidase subunit 1 (COI) sequences and traditional morphological approaches and then compared the results of these two methods. We successfully obtained COI sequences for 312 specimens belonging to 124 species, providing a DNA barcode reference library for nearly 50% of the currently known ant fauna of INP. Our results evidenced a clear barcode gap for all but two species, with a mean intraspecific divergence of 0.72%, and an average congeneric distance of 17.25%. Congruently, the library assembled here was remarkably useful for the discrimination of the ants of INP and even allowed us to link unidentified males and queens to their worker castes. To detect overlooked diversity, we classified the DNA barcodes into Molecular Operational Taxonomic Units (MOTUs) using three different clustering algorithms and then compared their number and composition to that of reference species identified based on morphology. The MOTU count was always higher than that of reference species regardless of the method, suggesting that the diversity of ants at INP could be between 6% and 10% higher than currently recognized. Lastly, our survey contributed with 78 new barcode clusters to the global DNA barcode reference library, and added 36 new records of ant species for the INP, being 23 of them new citations for Argentina.
Fil: Hanisch, Priscila Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Lavinia Oblanca, Pablo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Suarez, Andrew. University of Illinois; Estados Unidos
Fil: Lijtmaer, Dario Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Leponce, Maurice. Royal Belgian Institute of Natural Sciences; Bélgica
Fil: Paris, Carolina Ivon. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Tubaro, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
description Understanding patterns of species diversity rely on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the southernmost extent of this forest. We assessed ant diversity using both cytochrome c oxidase subunit 1 (COI) sequences and traditional morphological approaches and then compared the results of these two methods. We successfully obtained COI sequences for 312 specimens belonging to 124 species, providing a DNA barcode reference library for nearly 50% of the currently known ant fauna of INP. Our results evidenced a clear barcode gap for all but two species, with a mean intraspecific divergence of 0.72%, and an average congeneric distance of 17.25%. Congruently, the library assembled here was remarkably useful for the discrimination of the ants of INP and even allowed us to link unidentified males and queens to their worker castes. To detect overlooked diversity, we classified the DNA barcodes into Molecular Operational Taxonomic Units (MOTUs) using three different clustering algorithms and then compared their number and composition to that of reference species identified based on morphology. The MOTU count was always higher than that of reference species regardless of the method, suggesting that the diversity of ants at INP could be between 6% and 10% higher than currently recognized. Lastly, our survey contributed with 78 new barcode clusters to the global DNA barcode reference library, and added 36 new records of ant species for the INP, being 23 of them new citations for Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48318
Hanisch, Priscila Elena; Lavinia Oblanca, Pablo Damián; Suarez, Andrew; Lijtmaer, Dario Alejandro; Leponce, Maurice; et al.; Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest; Wiley Blackwell Publishing, Inc; Ecology and Evolution; 7; 23; 12-2017; 10451-10466
2045-7758
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48318
identifier_str_mv Hanisch, Priscila Elena; Lavinia Oblanca, Pablo Damián; Suarez, Andrew; Lijtmaer, Dario Alejandro; Leponce, Maurice; et al.; Mind the gap! Integrating taxonomic approaches to assess ant diversity at the southern extreme of the Atlantic Forest; Wiley Blackwell Publishing, Inc; Ecology and Evolution; 7; 23; 12-2017; 10451-10466
2045-7758
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.3549
info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.3549
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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