Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis

Autores
Pérez, María Rita; Pellegrini, Elisabeth; Cano Nicolau, Joel; Gueguen, Marie Madelaine; Le Guillou, Dounia Menouer; Merot, Yohann; Vaillant, Colette; Somoza, Gustavo Manuel; Kah, Olivier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2′-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.
Fil: Pérez, María Rita. Universite de Rennes I; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Pellegrini, Elisabeth. Universite de Rennes I; Francia
Fil: Cano Nicolau, Joel. Universite de Rennes I; Francia
Fil: Gueguen, Marie Madelaine. Universite de Rennes I; Francia
Fil: Le Guillou, Dounia Menouer. Universite de Rennes I; Francia
Fil: Merot, Yohann. Universite de Rennes I; Francia
Fil: Vaillant, Colette. Universite de Rennes I; Francia
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Kah, Olivier. Universite de Rennes I; Francia
Materia
Serotonin
Radial Glial Cells
Hypothalamus
Dopamine
Fish
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24261
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24261
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesisPérez, María RitaPellegrini, ElisabethCano Nicolau, JoelGueguen, Marie MadelaineLe Guillou, Dounia MenouerMerot, YohannVaillant, ColetteSomoza, Gustavo ManuelKah, OlivierSerotoninRadial Glial CellsHypothalamusDopamineFishhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2′-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.Fil: Pérez, María Rita. Universite de Rennes I; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Pellegrini, Elisabeth. Universite de Rennes I; FranciaFil: Cano Nicolau, Joel. Universite de Rennes I; FranciaFil: Gueguen, Marie Madelaine. Universite de Rennes I; FranciaFil: Le Guillou, Dounia Menouer. Universite de Rennes I; FranciaFil: Merot, Yohann. Universite de Rennes I; FranciaFil: Vaillant, Colette. Universite de Rennes I; FranciaFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Kah, Olivier. Universite de Rennes I; FranciaWiley Blackwell Publishing, Inc2013-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24261Pérez, María Rita; Pellegrini, Elisabeth; Cano Nicolau, Joel; Gueguen, Marie Madelaine; Le Guillou, Dounia Menouer; et al.; Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 38; 29-7-2013; 3292-33010953-816XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ejn.12348info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ejn.12348/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:48Zoai:ri.conicet.gov.ar:11336/24261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:49.089CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
title Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
spellingShingle Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
Pérez, María Rita
Serotonin
Radial Glial Cells
Hypothalamus
Dopamine
Fish
title_short Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
title_full Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
title_fullStr Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
title_full_unstemmed Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
title_sort Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
dc.creator.none.fl_str_mv Pérez, María Rita
Pellegrini, Elisabeth
Cano Nicolau, Joel
Gueguen, Marie Madelaine
Le Guillou, Dounia Menouer
Merot, Yohann
Vaillant, Colette
Somoza, Gustavo Manuel
Kah, Olivier
author Pérez, María Rita
author_facet Pérez, María Rita
Pellegrini, Elisabeth
Cano Nicolau, Joel
Gueguen, Marie Madelaine
Le Guillou, Dounia Menouer
Merot, Yohann
Vaillant, Colette
Somoza, Gustavo Manuel
Kah, Olivier
author_role author
author2 Pellegrini, Elisabeth
Cano Nicolau, Joel
Gueguen, Marie Madelaine
Le Guillou, Dounia Menouer
Merot, Yohann
Vaillant, Colette
Somoza, Gustavo Manuel
Kah, Olivier
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Serotonin
Radial Glial Cells
Hypothalamus
Dopamine
Fish
topic Serotonin
Radial Glial Cells
Hypothalamus
Dopamine
Fish
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2′-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.
Fil: Pérez, María Rita. Universite de Rennes I; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Pellegrini, Elisabeth. Universite de Rennes I; Francia
Fil: Cano Nicolau, Joel. Universite de Rennes I; Francia
Fil: Gueguen, Marie Madelaine. Universite de Rennes I; Francia
Fil: Le Guillou, Dounia Menouer. Universite de Rennes I; Francia
Fil: Merot, Yohann. Universite de Rennes I; Francia
Fil: Vaillant, Colette. Universite de Rennes I; Francia
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Kah, Olivier. Universite de Rennes I; Francia
description In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5-HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5-HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5-bromo-2′-deoxyuridine indicate that RGCs proliferate and give birth to 5-HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5-HT appears to promote genesis of 5-HT neurons that accumulate along the brain ventricles and contact the CSF.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24261
Pérez, María Rita; Pellegrini, Elisabeth; Cano Nicolau, Joel; Gueguen, Marie Madelaine; Le Guillou, Dounia Menouer; et al.; Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 38; 29-7-2013; 3292-3301
0953-816X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24261
identifier_str_mv Pérez, María Rita; Pellegrini, Elisabeth; Cano Nicolau, Joel; Gueguen, Marie Madelaine; Le Guillou, Dounia Menouer; et al.; Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 38; 29-7-2013; 3292-3301
0953-816X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ejn.12348
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ejn.12348/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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