Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats

Autores
Ríos Lugo, María J.; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Spinedi, Eduardo Julio; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n = 16 per group): (i) control diet (3% fat); (ii) highfat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in highfat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.
Fil: Ríos Lugo, María J.. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid. Facultad de Medicina; España
Materia
Adiponectin
Food Intake
Gene Expression
High-Fat Diet
Leptin
Medial Basal Hypothalamus
Melatonin
Neuropeptide Y
Prolactin-Releasing Peptide
Proopiomelanocortin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10801
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed ratsRíos Lugo, María J.Jiménez Ortega, VanesaCano Barquilla, PilarFernández Mateos, PilarSpinedi, Eduardo JulioCardinali, Daniel PedroEsquifino, Ana I.AdiponectinFood IntakeGene ExpressionHigh-Fat DietLeptinMedial Basal HypothalamusMelatoninNeuropeptide YProlactin-Releasing PeptideProopiomelanocortinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n = 16 per group): (i) control diet (3% fat); (ii) highfat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in highfat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.Fil: Ríos Lugo, María J.. Universidad Complutense de Madrid. Facultad de Medicina; EspañaFil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid. Facultad de Medicina; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; EspañaFil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esquifino, Ana I.. Universidad Complutense de Madrid. Facultad de Medicina; EspañaDe Gruyter2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10801Ríos Lugo, María J.; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Spinedi, Eduardo Julio; et al.; Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats; De Gruyter; Hormone Molecular Biology and Clinical Investigation; 21; 3; 3-2015; 175-1831868-1891enginfo:eu-repo/semantics/altIdentifier/doi/10.1515/hmbci-2014-0041info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/hmbci.2015.21.issue-3/hmbci-2014-0041/hmbci-2014-0041.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:45Zoai:ri.conicet.gov.ar:11336/10801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:45.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
spellingShingle Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
Ríos Lugo, María J.
Adiponectin
Food Intake
Gene Expression
High-Fat Diet
Leptin
Medial Basal Hypothalamus
Melatonin
Neuropeptide Y
Prolactin-Releasing Peptide
Proopiomelanocortin
title_short Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_full Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_fullStr Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_full_unstemmed Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_sort Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
dc.creator.none.fl_str_mv Ríos Lugo, María J.
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Fernández Mateos, Pilar
Spinedi, Eduardo Julio
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Ríos Lugo, María J.
author_facet Ríos Lugo, María J.
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Fernández Mateos, Pilar
Spinedi, Eduardo Julio
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Fernández Mateos, Pilar
Spinedi, Eduardo Julio
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Adiponectin
Food Intake
Gene Expression
High-Fat Diet
Leptin
Medial Basal Hypothalamus
Melatonin
Neuropeptide Y
Prolactin-Releasing Peptide
Proopiomelanocortin
topic Adiponectin
Food Intake
Gene Expression
High-Fat Diet
Leptin
Medial Basal Hypothalamus
Melatonin
Neuropeptide Y
Prolactin-Releasing Peptide
Proopiomelanocortin
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n = 16 per group): (i) control diet (3% fat); (ii) highfat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in highfat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.
Fil: Ríos Lugo, María J.. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid. Facultad de Medicina; España
Fil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid. Facultad de Medicina; España
description Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n = 16 per group): (i) control diet (3% fat); (ii) highfat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in highfat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10801
Ríos Lugo, María J.; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Spinedi, Eduardo Julio; et al.; Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats; De Gruyter; Hormone Molecular Biology and Clinical Investigation; 21; 3; 3-2015; 175-183
1868-1891
url http://hdl.handle.net/11336/10801
identifier_str_mv Ríos Lugo, María J.; Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Spinedi, Eduardo Julio; et al.; Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats; De Gruyter; Hormone Molecular Biology and Clinical Investigation; 21; 3; 3-2015; 175-183
1868-1891
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/hmbci-2014-0041
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/hmbci.2015.21.issue-3/hmbci-2014-0041/hmbci-2014-0041.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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